OBJECTIVE

To investigate the effects of rhGH and IGF-1 on the CD14 mRNA expression and cytokine secretion of peritoneal macrophages (Mphi) in scalded rats.

METHODS

Reverse-transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosordent assay (ELISA) were employed to observe the postburn changes of CD14 expression and cytokines secretion of the Mphi treated in vivo and in vitro by rhGH and IGF-1.

RESULTS

It was demonstrated that in vivo postburn application of rhGH and IGF-1 could enhance the transcription of CD14 mRNA and the secretion of cytokines from the peritoneal Mphi in mice. But it seemed that there was no additive effect between the two factors. Additionally, rhGH (at 40 ng/ml) and IGF-1 (at all tested concentrations) could activate the secretion of TNF and IL-6 from cultured mice Mphi in vitro.

CONCLUSIONS

Postburn application of rhGH might activate Mphi by IGF-1 induction, enhancing Mphi CD14 expression, which promoted more secretion of cytokines, and promote the enhancement of immune function.