Mouse Gene Mthfsd (ENSMUST00000116415.8) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus methenyltetrahydrofolate synthetase domain containing (Mthfsd), transcript variant 1, mRNA. (from RefSeq NM_001166482)
Gencode Transcript: ENSMUST00000116415.8
Gencode Gene: ENSMUSG00000031816.15
Transcript (Including UTRs)
   Position: mm10 chr8:121,097,553-121,108,327 Size: 10,775 Total Exon Count: 9 Strand: -
Coding Region
   Position: mm10 chr8:121,098,712-121,108,303 Size: 9,592 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther NamesModel Information
Methods
Data last updated at UCSC: 2019-09-20

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:121,097,553-121,108,327)mRNA (may differ from genome)Protein (372 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedSOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: MTHSD_MOUSE
DESCRIPTION: RecName: Full=Methenyltetrahydrofolate synthase domain-containing protein;
SIMILARITY: Contains 1 RRM (RNA recognition motif) domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -8.2024-0.342 Picture PostScript Text
3' UTR -126.00377-0.334 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002698 - FTHF_cligase
IPR024185 - FTHF_cligase-like
IPR012677 - Nucleotide-bd_a/b_plait
IPR000504 - RRM_dom

Pfam Domains:
PF01812 - 5-formyltetrahydrofolate cyclo-ligase family

SCOP Domains:
100950 - NagB/RpiA/CoA transferase-like
54928 - RNA-binding domain, RBD

ModBase Predicted Comparative 3D Structure on Q3URQ7
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserNo orthologNo ortholog
Gene Details     
Gene Sorter     
GenBank EnsemblEnsembl  
Protein SequenceProtein Sequence Protein Sequence  
AlignmentAlignment Alignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003676 nucleic acid binding
GO:0003723 RNA binding

Biological Process:
GO:0008298 intracellular mRNA localization

Cellular Component:
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  BC023782 - Mus musculus methenyltetrahydrofolate synthetase domain containing, mRNA (cDNA clone IMAGE:5346976), with apparent retained intron.
AK141278 - Mus musculus 7 days embryo whole body cDNA, RIKEN full-length enriched library, clone:C430017P21 product:hypothetical 5-formyltetrahydrofolate cyclo-ligase/RNA-binding region RNP-1 (RNA recognition motif) containing protein, full insert sequence.
AK195792 - Mus musculus cDNA, clone:Y1G0119K09, strand:unspecified.
AK195704 - Mus musculus cDNA, clone:Y1G0119F21, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000047282, based on BLAT search.
BC052066 - Mus musculus methenyltetrahydrofolate synthetase domain containing, mRNA (cDNA clone MGC:62465 IMAGE:5708660), complete cds.
BC026652 - Mus musculus methenyltetrahydrofolate synthetase domain containing, mRNA (cDNA clone IMAGE:4987772), with apparent retained intron.
AK083317 - Mus musculus 2 days neonate thymus thymic cells cDNA, RIKEN full-length enriched library, clone:C920004D05 product:similar to THYRO1001661 PROTEIN [Homo sapiens], full insert sequence.
AK188342 - Mus musculus cDNA, clone:Y0G0144N09, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000047282, based on BLAT search.

-  Other Names for This Gene
  Alternate Gene Symbols: MTHSD_MOUSE, NM_001166482, Q3URQ7, Q80WS4, Q8BUM0, uc009nro.1, uc009nro.2
UCSC ID: uc009nro.2
RefSeq Accession: NM_001166482
Protein: Q3URQ7 (aka MTHSD_MOUSE)
CCDS: CCDS52691.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.