Mouse Gene Rhag (ENSMUST00000024721.7) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus Rhesus blood group-associated A glycoprotein (Rhag), mRNA. (from RefSeq NM_011269)
Gencode Transcript: ENSMUST00000024721.7
Gencode Gene: ENSMUSG00000023926.8
Transcript (Including UTRs)
   Position: mm10 chr17:40,811,126-40,840,754 Size: 29,629 Total Exon Count: 10 Strand: +
Coding Region
   Position: mm10 chr17:40,811,176-40,839,782 Size: 28,607 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-20

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:40,811,126-40,840,754)mRNA (may differ from genome)Protein (438 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: RHAG_MOUSE
DESCRIPTION: RecName: Full=Ammonium transporter Rh type A; AltName: Full=Erythrocyte membrane glycoprotein Rh50; AltName: Full=Rhesus blood group family type A glycoprotein; Short=Rh family type A glycoprotein; Short=Rh type A glycoprotein; AltName: CD_antigen=CD241;
FUNCTION: May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane (By similarity).
SUBUNIT: Heterotetramer (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (By similarity).
SIMILARITY: Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -7.4050-0.148 Picture PostScript Text
3' UTR -221.10972-0.227 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR024041 - NH4_transpt_AmtB-like
IPR002229 - RhesusRHD

Pfam Domains:
PF00909 - Ammonium Transporter Family

SCOP Domains:
111352 - Ammonium transporter

ModBase Predicted Comparative 3D Structure on Q9QUT0
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologGenome BrowserNo ortholog
Gene Details     
Gene Sorter     
 RGDEnsembl WormBase 
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008519 ammonium transmembrane transporter activity
GO:0022840 leak channel activity
GO:0030506 ankyrin binding

Biological Process:
GO:0006873 cellular ion homeostasis
GO:0015670 carbon dioxide transport
GO:0015672 monovalent inorganic cation transport
GO:0015695 organic cation transport
GO:0015696 ammonium transport
GO:0048821 erythrocyte development
GO:0060586 multicellular organismal iron ion homeostasis
GO:0072488 ammonium transmembrane transport

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK157847 - Mus musculus erythroblast cDNA, RIKEN full-length enriched library, clone:K0C0023F15 product:Rhesus blood group-associated A glycoprotein, full insert sequence.
AK143851 - Mus musculus 6 days neonate spleen cDNA, RIKEN full-length enriched library, clone:F430206D18 product:Rhesus blood group-associated A glycoprotein, full insert sequence.
AF057526 - Mus musculus erythrocyte membrane protein Rh50 (Rhag) mRNA, complete cds.
BC103662 - Mus musculus Rhesus blood group-associated A glycoprotein, mRNA (cDNA clone MGC:123380 IMAGE:40039075), complete cds.
BC101941 - Mus musculus Rhesus blood group-associated A glycoprotein, mRNA (cDNA clone MGC:123378 IMAGE:40039068), complete cds.
BC101942 - Mus musculus Rhesus blood group-associated A glycoprotein, mRNA (cDNA clone MGC:123379 IMAGE:40039073), complete cds.
BC103512 - Mus musculus Rhesus blood group-associated A glycoprotein, mRNA (cDNA clone MGC:123381 IMAGE:40039076), complete cds.
AB015192 - Mus musculus domesticus Rh50 mRNA for 50 kD glycoprotein, complete cds, strain: C57BL-10SnJ.
AB015193 - Mus musculus brevirostris Rh50 mRNA for 50 kD glycoprotein, complete cds, strain: BFM-2MsfB6C3FI.
AF065395 - Mus musculus Rh50-like protein mRNA, complete cds.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9QUT0 (Reactome details) participates in the following event(s):

R-MMU-444416 RHAG transports NH4+ from cytosol to extracellular region (red blood cells)
R-MMU-1237069 RhAG passively translocates carbon dioxide from the extracellular region to the cytosol
R-MMU-1247645 RhAG passively translocates carbon dioxide from the cytosol to the extracellular region
R-MMU-444411 Rhesus glycoproteins mediate ammonium transport.
R-MMU-1237044 Erythrocytes take up carbon dioxide and release oxygen
R-MMU-1247673 Erythrocytes take up oxygen and release carbon dioxide
R-MMU-425366 Transport of bile salts and organic acids, metal ions and amine compounds
R-MMU-425428 Amine compound SLC transporters
R-MMU-1480926 O2/CO2 exchange in erythrocytes
R-MMU-425407 SLC-mediated transmembrane transport
R-MMU-382551 Transport of small molecules

-  Other Names for This Gene
  Alternate Gene Symbols: NM_011269, Q3UP27, Q794G1, Q9QUT0, Rh50, RHAG_MOUSE, uc008col.1, uc008col.2
UCSC ID: uc008col.2
RefSeq Accession: NM_011269
Protein: Q9QUT0 (aka RHAG_MOUSE)
CCDS: CCDS28787.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.