ID:ZN143_HUMAN DESCRIPTION: RecName: Full=Zinc finger protein 143; AltName: Full=SPH-binding factor; AltName: Full=Selenocysteine tRNA gene transcription-activating factor; Short=hStaf; FUNCTION: Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8. SUBUNIT: Interacts with CHD8. SUBCELLULAR LOCATION: Nucleus (Potential). TISSUE SPECIFICITY: Expressed in all tissues tested, with the strongest expression in ovary. SIMILARITY: Belongs to the GLI C2H2-type zinc-finger protein family. SIMILARITY: Contains 7 C2H2-type zinc fingers. SEQUENCE CAUTION: Sequence=AAC50266.1; Type=Erroneous initiation; Sequence=AAH20219.1; Type=Erroneous initiation; Sequence=BAF83822.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P52747
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding GO:0001077 transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding GO:0003676 nucleic acid binding GO:0003677 DNA binding GO:0005515 protein binding GO:0046872 metal ion binding
Biological Process: GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006357 regulation of transcription from RNA polymerase II promoter GO:0006359 regulation of transcription from RNA polymerase III promoter GO:0006366 transcription from RNA polymerase II promoter GO:0006383 transcription from RNA polymerase III promoter GO:0042795 snRNA transcription from RNA polymerase II promoter GO:0045944 positive regulation of transcription from RNA polymerase II promoter
BC020219 - Homo sapiens zinc finger protein 143, mRNA (cDNA clone MGC:31910 IMAGE:4902668), complete cds. AK313330 - Homo sapiens cDNA, FLJ93852. AK291133 - Homo sapiens cDNA FLJ76816 complete cds, highly similar to Homo sapiens zinc finger protein 143 (clone pHZ-1) (ZNF143), mRNA. AK297214 - Homo sapiens cDNA FLJ51632 complete cds, highly similar to Zinc finger protein 143. BC014458 - Homo sapiens cDNA clone IMAGE:4899390, containing frame-shift errors. AB527470 - Synthetic construct DNA, clone: pF1KB7874, Homo sapiens ZNF143 gene for zinc finger protein 143, without stop codon, in Flexi system. U09850 - Human zinc finger protein (ZNF143) mRNA, complete cds. EU446917 - Synthetic construct Homo sapiens clone IMAGE:100070040; IMAGE:100012126; FLH258295.01L zinc finger protein 143 (ZNF143) gene, encodes complete protein. AF071771 - Homo sapiens SPH-binding factor mRNA, partial cds. JD207127 - Sequence 188151 from Patent EP1572962. JD326217 - Sequence 307241 from Patent EP1572962. JD510140 - Sequence 491164 from Patent EP1572962. JD302569 - Sequence 283593 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein P52747 (Reactome details) participates in the following event(s):
R-HSA-83791 Binding of SNAPc, Oct-1, and Staf to Type 3 Promoter R-HSA-6807496 POU2F1 (OCT1) or POU2F2 (OCT2), SP1, and ZNF143 (STAF) bind the DSE of snRNA gene (U1, U2, U4, U4atac, U5, U11, U12) R-HSA-83793 Binding of TFIIIB to SNAPc:Oct-1:Staf:Type 3 Promoter Complex R-HSA-112054 RNA Polymerase III Abortive Initiation At Type 3 Open Promoters R-HSA-112156 RNA Polymerase III Simple Start Sequence Initiation At Type 3 Promoters R-HSA-112152 RNA Polymerase III Promoter Opening at Type 3 Promoters R-HSA-6810239 SNAPc binds PSE of snRNA gene (U1, U2, U4, U4atac, U5, U11, U12) R-HSA-83803 Recruitment of RNA Polymerase III to TFIIIB:SNAPc:Type 3 Promoter Complex R-HSA-6810234 General transcription factors bind SNAPc:POU2F1:ZNF143:snRNA gene R-HSA-6810233 CDK7 phosphorylates serine-5 and serine-7 of heptad repeats in C-terminal domain of RNA polymerase II at snRNA promoter R-HSA-6810238 RNA polymerase II binds initiation factors at promoter of snRNA gene (U1, U2, U4, U4atac, U5, U11, U12) R-HSA-6810235 RPAP2 binds RNA polymerase II phosphorylated at serine-7 residues of heptad repeats in the C-terminal domain R-HSA-6814885 CBCAP complex binds 7-methylguanosine cap of snRNA R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter R-HSA-6807505 RNA polymerase II transcribes snRNA genes R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation R-HSA-76046 RNA Polymerase III Transcription Initiation R-HSA-73857 RNA Polymerase II Transcription R-HSA-74158 RNA Polymerase III Transcription R-HSA-74160 Gene expression (Transcription)
US Server
Sequence and Links to Tools and Databases 
Microarray Expression Data 
