Description: Homo sapiens ubiquitin-conjugating enzyme E2K (UBE2K), transcript variant 1, mRNA. RefSeq Summary (NM_005339): The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]. Transcript (Including UTRs) Position: hg19 chr4:39,699,664-39,784,410 Size: 84,747 Total Exon Count: 7 Strand: + Coding Region Position: hg19 chr4:39,699,948-39,780,054 Size: 80,107 Coding Exon Count: 7
ID:UBE2K_HUMAN DESCRIPTION: RecName: Full=Ubiquitin-conjugating enzyme E2 K; EC=6.3.2.19; AltName: Full=Huntingtin-interacting protein 2; Short=HIP-2; AltName: Full=Ubiquitin carrier protein; AltName: Full=Ubiquitin-conjugating enzyme E2-25 kDa; Short=Ubiquitin-conjugating enzyme E2(25K); Short=Ubiquitin-conjugating enzyme E2-25K; AltName: Full=Ubiquitin-protein ligase; FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein- dependent degradation of RB1. CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine. PATHWAY: Protein modification; protein ubiquitination. SUBUNIT: Interacts with RNF138/NARF. Interacts with BRCA1. SUBCELLULAR LOCATION: Cytoplasm (By similarity). TISSUE SPECIFICITY: Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem. INDUCTION: By aggregated low-density lipoprotein. PTM: Sumoylation at Lys-14 impairs catalytic activity (By similarity). SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. SIMILARITY: Contains 1 UBA domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P61086
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006511 ubiquitin-dependent protein catabolic process GO:0010800 positive regulation of peptidyl-threonine phosphorylation GO:0010994 free ubiquitin chain polymerization GO:0016567 protein ubiquitination GO:0032434 regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0035458 cellular response to interferon-beta GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process GO:0060340 positive regulation of type I interferon-mediated signaling pathway GO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070936 protein K48-linked ubiquitination
JA482240 - Sequence 223 from Patent WO2011072091. JA482241 - Sequence 224 from Patent WO2011072091. JA482242 - Sequence 225 from Patent WO2011072091. JE980532 - Sequence 223 from Patent EP2862929. JE980533 - Sequence 224 from Patent EP2862929. JE980534 - Sequence 225 from Patent EP2862929. AK295846 - Homo sapiens cDNA FLJ57995 complete cds, moderately similar to Ubiquitin-conjugating enzyme E2-25 kDa (EC 6.3.2.19). AK315524 - Homo sapiens cDNA, FLJ96590, Homo sapiens huntingtin interacting protein 2 (HIP2), mRNA. AK291454 - Homo sapiens cDNA FLJ77142 complete cds, highly similar to Homo sapiens LIG mRNA for E2 ubiquitin-conjugating enzyme alternative splicing form. AK307130 - Homo sapiens cDNA, FLJ97078. AK093533 - Homo sapiens cDNA FLJ36214 fis, clone THYMU2000672, highly similar to Ubiquitin-conjugating enzyme E2-25 kDa (EC 6.3.2.19). BC022804 - Homo sapiens ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast), mRNA (cDNA clone MGC:39129 IMAGE:4893778), complete cds. BC050600 - Homo sapiens ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast), mRNA (cDNA clone MGC:60046 IMAGE:6502901), complete cds. AB022435 - Homo sapiens LIG mRNA for E2 ubiquitin-conjugating enzyme, complete cds. AB022436 - Homo sapiens LIG mRNA for E2 ubiquitin-conjugating enzyme alternative splicing form, complete cds. U58522 - Human huntingtin interacting protein (HIP2) mRNA, complete cds. CU690732 - Synthetic construct Homo sapiens gateway clone IMAGE:100021315 5' read HIP2 mRNA. AB527742 - Synthetic construct DNA, clone: pF1KB7927, Homo sapiens UBE2K gene for ubiquitin-conjugating enzyme E2K, without stop codon, in Flexi system. EU446896 - Synthetic construct Homo sapiens clone IMAGE:100069886; IMAGE:100012105; FLH257039.01L huntingtin interacting protein 2 (HIP2) gene, encodes complete protein. KJ891343 - Synthetic construct Homo sapiens clone ccsbBroadEn_00737 UBE2K gene, encodes complete protein. JD056625 - Sequence 37649 from Patent EP1572962. JD442383 - Sequence 423407 from Patent EP1572962. JD491677 - Sequence 472701 from Patent EP1572962. JD160577 - Sequence 141601 from Patent EP1572962. JD243527 - Sequence 224551 from Patent EP1572962. JD440385 - Sequence 421409 from Patent EP1572962. JD435671 - Sequence 416695 from Patent EP1572962. JD559721 - Sequence 540745 from Patent EP1572962. JD327448 - Sequence 308472 from Patent EP1572962. JD357081 - Sequence 338105 from Patent EP1572962. JD058717 - Sequence 39741 from Patent EP1572962. JD552042 - Sequence 533066 from Patent EP1572962. BX641089 - Homo sapiens mRNA; cDNA DKFZp686J24237 (from clone DKFZp686J24237). JD022677 - Sequence 3701 from Patent EP1572962. JD031452 - Sequence 12476 from Patent EP1572962. JD031604 - Sequence 12628 from Patent EP1572962. AL117568 - Homo sapiens mRNA; cDNA DKFZp564C1216 (from clone DKFZp564C1216).
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa04120 - Ubiquitin mediated proteolysis
Reactome (by CSHL, EBI, and GO)
Protein P61086 (Reactome details) participates in the following event(s):
R-HSA-8852129 UBA1 conjugates ubiquitin to cytosolic E2 enzymes R-HSA-983152 Transfer of ubiquitin from E1 to E2 R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2 R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex R-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-HSA-936440 Negative regulators of DDX58/IFIH1 signaling R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation R-HSA-8852135 Protein ubiquitination R-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta R-HSA-983169 Class I MHC mediated antigen processing & presentation R-HSA-597592 Post-translational protein modification R-HSA-168249 Innate Immune System R-HSA-1280218 Adaptive Immune System R-HSA-392499 Metabolism of proteins R-HSA-168256 Immune System