Description: Homo sapiens dynactin 3 (p22) (DCTN3), transcript variant 1, mRNA. RefSeq Summary (NM_007234): This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]. Transcript (Including UTRs) Position: hg19 chr9:34,613,548-34,620,496 Size: 6,949 Total Exon Count: 7 Strand: - Coding Region Position: hg19 chr9:34,613,779-34,620,461 Size: 6,683 Coding Exon Count: 7
ID:DCTN3_HUMAN DESCRIPTION: RecName: Full=Dynactin subunit 3; AltName: Full=Dynactin complex subunit 22 kDa subunit; Short=p22; FUNCTION: Together with dynein may be involved in spindle assembly and cytokinesis. SUBUNIT: Subunit of dynactin, a multiprotein complex associated with dynein. INTERACTION: P40425:PBX2; NbExp=1; IntAct=EBI-347442, EBI-348489; SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody. Note=Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells. TISSUE SPECIFICITY: Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain. SIMILARITY: Belongs to the dynactin subunit 3 family. SEQUENCE CAUTION: Sequence=CAI13144.1; Type=Erroneous initiation; Sequence=CAI14178.1; Type=Erroneous gene model prediction; Sequence=CAI14180.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O75935
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0005198 structural molecule activity GO:0005515 protein binding
Biological Process: GO:0000086 G2/M transition of mitotic cell cycle GO:0000278 mitotic cell cycle GO:0006888 ER to Golgi vesicle-mediated transport GO:0007017 microtubule-based process GO:0007049 cell cycle GO:0010389 regulation of G2/M transition of mitotic cell cycle GO:0019886 antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0051301 cell division GO:0061640 cytoskeleton-dependent cytokinesis GO:0097711 ciliary basal body docking
BC000319 - Homo sapiens dynactin 3 (p22), mRNA (cDNA clone MGC:8470 IMAGE:2821720), complete cds. BC003004 - Homo sapiens dynactin 3 (p22), mRNA (cDNA clone MGC:4002 IMAGE:2821720), complete cds. AF082513 - Homo sapiens dynactin subunit (p22) mRNA, complete cds. AK091661 - Homo sapiens cDNA FLJ34342 fis, clone FEBRA2009986, moderately similar to Dynactin 3 (p22); dynactin light chain. AX747117 - Sequence 642 from Patent EP1308459. BC107697 - Homo sapiens dynactin 3 (p22), mRNA (cDNA clone MGC:111190 IMAGE:6454606), complete cds. AK055199 - Homo sapiens cDNA FLJ30637 fis, clone CTONG2002590, weakly similar to Dynactin 3 (p22); dynactin light chain. JD044890 - Sequence 25914 from Patent EP1572962. JD475369 - Sequence 456393 from Patent EP1572962. JD304157 - Sequence 285181 from Patent EP1572962. JD217172 - Sequence 198196 from Patent EP1572962. JD495035 - Sequence 476059 from Patent EP1572962. JD311408 - Sequence 292432 from Patent EP1572962. AK314730 - Homo sapiens cDNA, FLJ95591, Homo sapiens dynactin 3 (p22) (DCTN3), transcript variant 1, mRNA. AB463589 - Synthetic construct DNA, clone: pF1KB6822, Homo sapiens DCTN3 gene for dynactin 3, without stop codon, in Flexi system. CR541889 - Homo sapiens full open reading frame cDNA clone RZPDo834A0733D for gene DCTN3, dynactin 3 (p22); complete cds, without stopcodon. JD358222 - Sequence 339246 from Patent EP1572962. JD273543 - Sequence 254567 from Patent EP1572962. JD049263 - Sequence 30287 from Patent EP1572962. KJ893266 - Synthetic construct Homo sapiens clone ccsbBroadEn_02660 DCTN3 gene, encodes complete protein. DQ891980 - Synthetic construct clone IMAGE:100004610; FLH182040.01X; RZPDo839B12138D dynactin 3 (p22) (DCTN3) gene, encodes complete protein. EU176665 - Synthetic construct Homo sapiens clone IMAGE:100011455; FLH182039.01L; RZPDo839G12254D dynactin 3 (p22) (DCTN3) gene, encodes complete protein. CU674154 - Synthetic construct Homo sapiens gateway clone IMAGE:100017968 5' read DCTN3 mRNA.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_Lis1Pathway - Lissencephaly gene (LIS1) in neuronal migration and development
Reactome (by CSHL, EBI, and GO)
Protein O75935 (Reactome details) participates in the following event(s):
R-HSA-380272 Plk1-mediated phosphorylation of Nlp R-HSA-380283 Recruitment of additional gamma tubulin/ gamma TuRC to the centrosome R-HSA-380294 Loss of C-Nap-1 from centrosomes R-HSA-380311 Recruitment of Plk1 to centrosomes R-HSA-380455 Recruitment of CDK11p58 to the centrosomes R-HSA-380303 Dissociation of Phospho-Nlp from the centrosome R-HSA-5626220 C2CD3 binds the mother centriole R-HSA-380508 Translocation of NuMA to the centrosomes R-HSA-2574845 AJUBA binds centrosome-associated AURKA R-HSA-8853405 TPX2 binds AURKA at centrosomes R-HSA-3000319 BORA binds PLK1 and AURKA R-HSA-2574840 AJUBA facilitates AURKA autophosphorylation R-HSA-3000310 AURKA phosphorylates PLK1 R-HSA-5626223 C2CD3 and OFD1 recruit 5 distal appendage proteins to the centriole R-HSA-5626681 Recruitment of transition zone proteins R-HSA-5626227 CP110 and CEP97 dissociate from the centriole R-HSA-380316 Association of NuMA with microtubules R-HSA-8853419 TPX2 promotes AURKA autophosphorylation R-HSA-5626228 The distal appendage proteins recruit TTBK2 R-HSA-5638009 CEP164 recruits RAB3IP-carrying Golgi-derived vesicles to the basal body R-HSA-5626699 MARK4 binds ODF2 in the centriole R-HSA-5617816 RAB3IP stimulates nucleotide exchange on RAB8A R-HSA-6809003 ERGIC-to-Golgi vesicles bind dynein:dynactin R-HSA-6809006 Vesicle is tethered through binding GOLGA2:GORASP1, GOLGB1 and the COG complex R-HSA-8849350 RAB6:GTP displaces PAFAH1B1 from dynein:dynactin complex R-HSA-380259 Loss of Nlp from mitotic centrosomes R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-5620912 Anchoring of the basal body to the plasma membrane R-HSA-380320 Recruitment of NuMA to mitotic centrosomes R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition R-HSA-8854518 AURKA Activation by TPX2 R-HSA-380287 Centrosome maturation R-HSA-5617833 Cilium Assembly R-HSA-68877 Mitotic Prometaphase R-HSA-69275 G2/M Transition R-HSA-1852241 Organelle biogenesis and maintenance R-HSA-68886 M Phase R-HSA-453274 Mitotic G2-G2/M phases R-HSA-69278 Cell Cycle (Mitotic) R-HSA-2132295 MHC class II antigen presentation R-HSA-6807878 COPI-mediated anterograde transport R-HSA-6811436 COPI-independent Golgi-to-ER retrograde traffic R-HSA-1640170 Cell Cycle R-HSA-1280218 Adaptive Immune System R-HSA-199977 ER to Golgi Anterograde Transport R-HSA-8856688 Golgi-to-ER retrograde transport R-HSA-168256 Immune System R-HSA-199991 Membrane Trafficking R-HSA-948021 Transport to the Golgi and subsequent modification R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic R-HSA-5653656 Vesicle-mediated transport R-HSA-446203 Asparagine N-linked glycosylation R-HSA-597592 Post-translational protein modification R-HSA-392499 Metabolism of proteins
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