Human Gene DNAJB3 (uc002vuz.3)
  Description: Homo sapiens DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr2:234,651,396-234,652,661 Size: 1,266 Total Exon Count: 1 Strand: -
Coding Region
   Position: hg19 chr2:234,652,125-234,652,562 Size: 438 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:234,651,396-234,652,661)mRNA (may differ from genome)Protein (145 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsH-INV
HGNCHPRDLynxMGIneXtProtPubMed
UniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DNJB3_HUMAN
DESCRIPTION: RecName: Full=DnaJ homolog subfamily B member 3;
FUNCTION: May operate as a co-chaperone of the male germ cell- and haploid stage-specific Hsp70 proteins (Potential).
TISSUE SPECIFICITY: Expressed in sperm (at protein level).
SIMILARITY: Contains 1 J domain.
CAUTION: Due to a premature stop codon, the human protein is truncated and lacks the DNAJB3 canonical C-terminal domain. However, expression studies suggest the existence of the mRNA and protein in human.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 32.15 RPKM in Testis
Total median expression: 33.45 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -54.2099-0.547 Picture PostScript Text
3' UTR -192.14729-0.264 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001623 - DnaJ_N
IPR018253 - Heat_shock_DnaJ_CS
IPR003095 - Hsp_DnaJ

Pfam Domains:
PF00226 - DnaJ domain
PF13446 - A repeated domain in UCH-protein

SCOP Domains:
46565 - Chaperone J-domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2EJ7 - NMR


ModBase Predicted Comparative 3D Structure on Q8WWF6
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
      
      
      

-  Descriptions from all associated GenBank mRNAs
  AY083665 - Homo sapiens HCG3 protein mRNA, complete cds.
BC017590 - Homo sapiens DnaJ (Hsp40) homolog, subfamily B, member 3, mRNA (cDNA clone MGC:26890 IMAGE:4828023), complete cds.
BC024013 - Homo sapiens DnaJ (Hsp40) homolog, subfamily B, member 3, mRNA (cDNA clone MGC:26879 IMAGE:4826480), complete cds.
LF318913 - JP 2014500723-A/126416: Polycomb-Associated Non-Coding RNAs.
HQ448310 - Synthetic construct Homo sapiens clone IMAGE:100071726; CCSB001480_02 HCG3 gene (HCG3) gene, encodes complete protein.
KJ896267 - Synthetic construct Homo sapiens clone ccsbBroadEn_05661 DNAJB3 gene, encodes complete protein.
KJ900752 - Synthetic construct Homo sapiens clone ccsbBroadEn_10146 DNAJB3 gene, encodes complete protein.
MA554490 - JP 2018138019-A/126416: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: DNJB3_HUMAN, HCG3, NM_001001394, NP_001001394, Q8WWF6
UCSC ID: uc002vuz.3
RefSeq Accession: NM_001001394
Protein: Q8WWF6 (aka DNJB3_HUMAN)

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001001394.3
exon count: 1CDS single in 3' UTR: no RNA size: 1266
ORF size: 438CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1045.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.