Description: Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46), mRNA. RefSeq Summary (NM_014829): This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]. Transcript (Including UTRs) Position: hg19 chr5:134,094,461-134,166,812 Size: 72,352 Total Exon Count: 23 Strand: + Coding Region Position: hg19 chr5:134,094,629-134,164,396 Size: 69,768 Coding Exon Count: 23
ID:DDX46_HUMAN DESCRIPTION: RecName: Full=Probable ATP-dependent RNA helicase DDX46; EC=3.6.4.13; AltName: Full=DEAD box protein 46; AltName: Full=PRP5 homolog; FUNCTION: Plays an essential role in splicing, either prior to, or during splicing A complex formation. CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. SUBUNIT: Integral component of the 17S U2 snRNP. SUBCELLULAR LOCATION: Nucleus speckle. Nucleus, Cajal body. Note=Present in Cajal bodies (CBs) and nuclear speckles. SIMILARITY: Belongs to the DEAD box helicase family. DDX46/PRP5 subfamily. SIMILARITY: Contains 1 helicase ATP-binding domain. SIMILARITY: Contains 1 helicase C-terminal domain. SEQUENCE CAUTION: Sequence=BAA34521.2; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q7L014
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK126011 - Homo sapiens cDNA FLJ44023 fis, clone TESTI4026510, highly similar to Homo sapiens RNA helicase. BC105598 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 46, mRNA (cDNA clone IMAGE:4714593), partial cds. AK291788 - Homo sapiens cDNA FLJ78679 complete cds, highly similar to Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46), mRNA. AF106680 - Homo sapiens RNA helicase mRNA, complete cds. AB018344 - Homo sapiens KIAA0801 mRNA for KIAA0801 protein. BC012304 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 46, mRNA (cDNA clone MGC:9936 IMAGE:3873814), complete cds. JD514166 - Sequence 495190 from Patent EP1572962. CU680282 - Synthetic construct Homo sapiens gateway clone IMAGE:100020513 5' read DDX46 mRNA. BX647426 - Homo sapiens mRNA; cDNA DKFZp686J23144 (from clone DKFZp686J23144). AK058058 - Homo sapiens cDNA FLJ25329 fis, clone TST00542. JD146756 - Sequence 127780 from Patent EP1572962. JD490645 - Sequence 471669 from Patent EP1572962. JD431377 - Sequence 412401 from Patent EP1572962. JD286873 - Sequence 267897 from Patent EP1572962. JD286874 - Sequence 267898 from Patent EP1572962. JD511652 - Sequence 492676 from Patent EP1572962. BC030755 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 46, mRNA (cDNA clone IMAGE:4797178). JD020421 - Sequence 1445 from Patent EP1572962. JD035572 - Sequence 16596 from Patent EP1572962. JD035395 - Sequence 16419 from Patent EP1572962.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa03040 - Spliceosome
Reactome (by CSHL, EBI, and GO)
Protein Q7L014 (Reactome details) participates in the following event(s):
R-HSA-72124 Formation of the Spliceosomal A Complex R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-72127 Formation of the Spliceosomal B Complex R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-156661 Formation of Exon Junction Complex R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-72172 mRNA Splicing R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-8953854 Metabolism of RNA