Description: Homo sapiens BUB3 mitotic checkpoint protein (BUB3), transcript variant 1, mRNA. RefSeq Summary (NM_004725): This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]. Transcript (Including UTRs) Position: hg19 chr10:124,913,760-124,924,886 Size: 11,127 Total Exon Count: 8 Strand: + Coding Region Position: hg19 chr10:124,914,434-124,923,351 Size: 8,918 Coding Exon Count: 7
ID:BUB3_HUMAN DESCRIPTION: RecName: Full=Mitotic checkpoint protein BUB3; FUNCTION: Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. SUBUNIT: Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. INTERACTION: Q5XI90:Dynlt3 (xeno); NbExp=2; IntAct=EBI-1050987, EBI-1781818; SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore (By similarity). Note=Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition (By similarity). PTM: Poly-ADP-ribosylated by PARP1 (By similarity). SIMILARITY: Belongs to the WD repeat BUB3 family. SIMILARITY: Contains 5 WD repeats.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O43684
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
BX647392 - Homo sapiens mRNA; cDNA DKFZp686J24144 (from clone DKFZp686J24144). AK312546 - Homo sapiens cDNA, FLJ92917, Homo sapiens BUB3 budding uninhibited by benzimidazoles 3 homolog(yeast) (BUB3), mRNA. AK307958 - Homo sapiens cDNA, FLJ97906. AK293255 - Homo sapiens cDNA FLJ57899 complete cds, highly similar to Mitotic checkpoint protein BUB3. AF081496 - Homo sapiens kinetochore protein BUB3 (BUB3) mRNA, complete cds. BC005138 - Homo sapiens budding uninhibited by benzimidazoles 3 homolog (yeast), mRNA (cDNA clone MGC:1527 IMAGE:3504520), complete cds. AF047472 - Homo sapiens spleen mitotic checkpoint BUB3 (BUB3) mRNA, complete cds. AF047473 - Homo sapiens testis mitotic checkpoint BUB3 (BUB3) mRNA, complete cds. BC022438 - Homo sapiens budding uninhibited by benzimidazoles 3 homolog (yeast), mRNA (cDNA clone MGC:24748 IMAGE:4281970), complete cds. AK226060 - Homo sapiens mRNA for BUB3 budding uninhibited by benzimidazoles 3 isoform a variant, clone: hg01710. AF053304 - Homo sapiens mitotic checkpoint component Bub3 (BUB3) mRNA, complete cds. HM005340 - Homo sapiens clone HTL-T-27 testicular tissue protein Li 27 mRNA, complete cds. CU692616 - Synthetic construct Homo sapiens gateway clone IMAGE:100022217 5' read BUB3 mRNA. AB463452 - Synthetic construct DNA, clone: pF1KB9385, Homo sapiens BUB3 gene for BUB3 budding uninhibited by benzimidazoles 3 homolog, without stop codon, in Flexi system. AB590162 - Synthetic construct DNA, clone: pFN21AE1557, Homo sapiens BUB3 gene for budding uninhibited by benzimidazoles 3 homolog, without stop codon, in Flexi system. DQ891852 - Synthetic construct clone IMAGE:100004482; FLH180614.01X; RZPDo839E02134D BUB3 budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3) gene, encodes complete protein. DQ895042 - Synthetic construct Homo sapiens clone IMAGE:100009502; FLH180610.01L; RZPDo839E02133D BUB3 budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3) gene, encodes complete protein. KJ892706 - Synthetic construct Homo sapiens clone ccsbBroadEn_02100 BUB3 gene, encodes complete protein. KJ897977 - Synthetic construct Homo sapiens clone ccsbBroadEn_07371 BUB3 gene, encodes complete protein. JD165124 - Sequence 146148 from Patent EP1572962. JD304942 - Sequence 285966 from Patent EP1572962. JD488945 - Sequence 469969 from Patent EP1572962. JD504011 - Sequence 485035 from Patent EP1572962. JD358229 - Sequence 339253 from Patent EP1572962. JD310900 - Sequence 291924 from Patent EP1572962. JD352191 - Sequence 333215 from Patent EP1572962. JD290514 - Sequence 271538 from Patent EP1572962. JD296806 - Sequence 277830 from Patent EP1572962. JD491254 - Sequence 472278 from Patent EP1572962. JD335551 - Sequence 316575 from Patent EP1572962.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa04110 - Cell cycle
Reactome (by CSHL, EBI, and GO)
Protein O43684 (Reactome details) participates in the following event(s):
R-HSA-141437 Formation of the MCC complex R-HSA-141423 Binding of the MCC complex to the APC/C complex R-HSA-174238 Activation of APC/C:Cdc20 by dissociation of Cdc20:phospho-APC/C from Cdc20:phospho-APC/C:Mad2:Bub3:BubR1 R-HSA-174171 Association of Cyclin A with the APC/C R-HSA-179410 Association of Nek2A with MCC:APC/C R-HSA-141409 Mad1 binds kinetochore R-HSA-375302 Kinetochore capture of astral microtubules R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2 R-HSA-174104 Ubiquitination of Cyclin A by APC/C:Cdc20 complex R-HSA-179417 Multiubiquitination of Nek2A R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex R-HSA-141439 Release of activated MAD2 from kinetochores R-HSA-2467811 Separation of sister chromatids R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1 R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres R-HSA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex R-HSA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins R-HSA-141405 Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-HSA-179409 APC-Cdc20 mediated degradation of Nek2A R-HSA-176814 Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-68877 Mitotic Prometaphase R-HSA-5663220 RHO GTPases Activate Formins R-HSA-69618 Mitotic Spindle Checkpoint R-HSA-176408 Regulation of APC/C activators between G1/S and early anaphase R-HSA-179419 APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint R-HSA-174143 APC/C-mediated degradation of cell cycle proteins R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-2467813 Separation of Sister Chromatids R-HSA-141424 Amplification of signal from the kinetochores R-HSA-68886 M Phase R-HSA-195258 RHO GTPase Effectors R-HSA-69620 Cell Cycle Checkpoints R-HSA-453276 Regulation of mitotic cell cycle R-HSA-68882 Mitotic Anaphase R-HSA-69278 Cell Cycle (Mitotic) R-HSA-194315 Signaling by Rho GTPases R-HSA-1640170 Cell Cycle R-HSA-2555396 Mitotic Metaphase and Anaphase R-HSA-162582 Signal Transduction
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