Description: Homo sapiens nuclear undecaprenyl pyrophosphate synthase 1 homolog (S. cerevisiae) (NUS1), mRNA. RefSeq Summary (NM_138459): This gene encodes a type I single transmembrane domain receptor, which is a subunit of cis-prenyltransferase, and serves as a specific receptor for the neural and cardiovascular regulator Nogo-B. The encoded protein is essential for dolichol synthesis and protein glycosylation. This gene is highly expressed in non-small cell lung carcinomas as well as estrogen receptor-alpha positive breast cancer cells where it promotes epithelial mesenchymal transition. This gene is associated with the poor prognosis of human hepatocellular carcinoma patients. Naturally occurring mutations in this gene cause a congenital disorder of glycosylation and are associated with epilepsy. A knockout of the orthologous gene in mice causes embryonic lethality before day 6.5. Pseudogenes of this gene have been defined on chromosomes 13 and X. [provided by RefSeq, May 2017]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Transcript (Including UTRs) Position: hg19 chr6:117,996,617-118,031,886 Size: 35,270 Total Exon Count: 5 Strand: + Coding Region Position: hg19 chr6:117,996,834-118,028,178 Size: 31,345 Coding Exon Count: 5
ID:NGBR_HUMAN DESCRIPTION: RecName: Full=Nogo-B receptor; Short=NgBR; AltName: Full=Nuclear undecaprenyl pyrophosphate synthase 1 homolog; Flags: Precursor; FUNCTION: Acts as a specific receptor for the N-terminus of Nogo- B, a neural and cardiovascular regulator. Regulates the stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol. FUNCTION: Essential component of the dolichol monophosphate (Dol- P) biosynthetic machinery. PATHWAY: Protein modification; protein glycosylation. SUBUNIT: Interacts with DHDDS, promoting its isoprenyltransferase activity. Interacts with NPC2. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note=Colocalizes with Nogo-B during VEGF and wound healing angiogenesis. MISCELLANEOUS: Although strongly related to UPP synthase family proteins, it has no lipid transferase activity. MISCELLANEOUS: NUS1 seems to exist in two topological orientations, a minor glycosylated species with its C-terminus oriented towards the lumen regulating NPC2 stability, and a major fraction oriented with its C-terminus directed towards the cytosol where it regulates cis-IPTase activity. SIMILARITY: Belongs to the UPP synthase family. SEQUENCE CAUTION: Sequence=AAB72234.1; Type=Frameshift; Positions=Several;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q96E22
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
BC066910 - Homo sapiens nuclear undecaprenyl pyrophosphate synthase 1 homolog (S. cerevisiae), mRNA (cDNA clone MGC:87099 IMAGE:4830860), complete cds. BC150654 - Homo sapiens nuclear undecaprenyl pyrophosphate synthase 1 homolog (S. cerevisiae), mRNA (cDNA clone MGC:183565 IMAGE:9057025), complete cds. BC150655 - Homo sapiens nuclear undecaprenyl pyrophosphate synthase 1 homolog (S. cerevisiae), mRNA (cDNA clone MGC:183566 IMAGE:9057026), complete cds. AK125119 - Homo sapiens cDNA FLJ43129 fis, clone CTONG3005648. BC110325 - Homo sapiens nuclear undecaprenyl pyrophosphate synthase 1 homolog (S. cerevisiae), mRNA (cDNA clone MGC:117249 IMAGE:5553827), complete cds. BC063794 - Homo sapiens nuclear undecaprenyl pyrophosphate synthase 1 homolog (S. cerevisiae), mRNA (cDNA clone MGC:70590 IMAGE:6500832), complete cds. AK126335 - Homo sapiens cDNA FLJ44363 fis, clone TRACH3008093. BC013026 - Homo sapiens nuclear undecaprenyl pyrophosphate synthase 1 homolog (S. cerevisiae), mRNA (cDNA clone MGC:4666 IMAGE:3531630), complete cds. JD498545 - Sequence 479569 from Patent EP1572962. KJ895302 - Synthetic construct Homo sapiens clone ccsbBroadEn_04696 NUS1 gene, encodes complete protein. AK130093 - Homo sapiens cDNA FLJ26583 fis, clone LNF07165. JD035544 - Sequence 16568 from Patent EP1572962. JD029889 - Sequence 10913 from Patent EP1572962. JD243911 - Sequence 224935 from Patent EP1572962. JD032420 - Sequence 13444 from Patent EP1572962. JD504594 - Sequence 485618 from Patent EP1572962. JD549145 - Sequence 530169 from Patent EP1572962. JD144884 - Sequence 125908 from Patent EP1572962. JD223681 - Sequence 204705 from Patent EP1572962. JD504358 - Sequence 485382 from Patent EP1572962. JD512909 - Sequence 493933 from Patent EP1572962. JD494398 - Sequence 475422 from Patent EP1572962. JD131228 - Sequence 112252 from Patent EP1572962. JD233675 - Sequence 214699 from Patent EP1572962. JD175057 - Sequence 156081 from Patent EP1572962. JD175058 - Sequence 156082 from Patent EP1572962. JD527209 - Sequence 508233 from Patent EP1572962. JD419782 - Sequence 400806 from Patent EP1572962. AJ420576 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 1657486. AK025467 - Homo sapiens cDNA: FLJ21814 fis, clone HEP01068. JD175057 - Sequence 156081 from Patent EP1572962. JD175058 - Sequence 156082 from Patent EP1572962. JD372368 - Sequence 353392 from Patent EP1572962. JD047016 - Sequence 28040 from Patent EP1572962. JD298777 - Sequence 279801 from Patent EP1572962. JD301869 - Sequence 282893 from Patent EP1572962. JD313877 - Sequence 294901 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q96E22 (Reactome details) participates in the following event(s):
R-HSA-4419978 DHDDS:NUS1 elongates E,E-FPP with (n)IPPP to form pPPP R-HSA-446199 Synthesis of Dolichyl-phosphate R-HSA-446219 Synthesis of substrates in N-glycan biosythesis R-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein R-HSA-446203 Asparagine N-linked glycosylation R-HSA-597592 Post-translational protein modification R-HSA-392499 Metabolism of proteins