Human Gene DNAJC24 (uc001msx.3)
  Description: Homo sapiens DnaJ (Hsp40) homolog, subfamily C, member 24 (DNAJC24), mRNA.
RefSeq Summary (NM_181706): Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM, Mar 2008]. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in other species. An alternative downstream start codon, which has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 1 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo.
Transcript (Including UTRs)
   Position: hg19 chr11:31,391,377-31,454,382 Size: 63,006 Total Exon Count: 5 Strand: +
Coding Region
   Position: hg19 chr11:31,392,296-31,451,948 Size: 59,653 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:31,391,377-31,454,382)mRNA (may differ from genome)Protein (149 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
H-INVHGNCHPRDLynxMalacardsMGI
neXtProtOMIMPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DJC24_HUMAN
DESCRIPTION: RecName: Full=DnaJ homolog subfamily C member 24; AltName: Full=CSL-type zinc finger-containing protein 3; AltName: Full=DPH4 homolog;
FUNCTION: Stimulates the ATPase activity of several Hsp70-type chaperones (By similarity).
SIMILARITY: Belongs to the DPH4 family.
SIMILARITY: Contains 1 DPH-type zinc finger.
SIMILARITY: Contains 1 J domain.
SEQUENCE CAUTION: Sequence=CAM28214.1; Type=Erroneous initiation; Sequence=EAW68245.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: DNAJC24
Diseases sorted by gene-association score: diphtheria (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 3.18 RPKM in Testis
Total median expression: 76.98 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -37.70116-0.325 Picture PostScript Text
3' UTR -857.982434-0.352 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001623 - DnaJ_N
IPR003095 - Hsp_DnaJ
IPR007872 - Znf_DHP

Pfam Domains:
PF00226 - DnaJ domain
PF05207 - CSL zinc finger

SCOP Domains:
46565 - Chaperone J-domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2L6L - NMR MuPIT


ModBase Predicted Comparative 3D Structure on Q6P3W2
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserNo orthologNo ortholog
Gene Details  Gene Details  
Gene Sorter  Gene Sorter  
  EnsemblFlyBase  
  Protein SequenceProtein Sequence  
  AlignmentAlignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001671 ATPase activator activity
GO:0008198 ferrous iron binding
GO:0008270 zinc ion binding
GO:0046872 metal ion binding

Biological Process:
GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine
GO:0032781 positive regulation of ATPase activity
GO:0055114 oxidation-reduction process
GO:0061077 chaperone-mediated protein folding

Cellular Component:
GO:0005737 cytoplasm
GO:0005856 cytoskeleton


-  Descriptions from all associated GenBank mRNAs
  AK123087 - Homo sapiens cDNA FLJ41092 fis, clone ASTRO2004877.
AL833128 - Homo sapiens mRNA; cDNA DKFZp313H1832 (from clone DKFZp313H1832).
AK289665 - Homo sapiens cDNA FLJ76516 complete cds, highly similar to Homo sapiens zinc finger, CSL-type containing 3 (ZCSL3), mRNA.
BC036571 - Homo sapiens DnaJ (Hsp40) homolog, subfamily C, member 24, mRNA (cDNA clone MGC:41914 IMAGE:4793909), complete cds.
BC044931 - Homo sapiens zinc finger, CSL-type containing 3, mRNA (cDNA clone IMAGE:6059077).
BC063804 - Homo sapiens DnaJ (Hsp40) homolog, subfamily C, member 24, mRNA (cDNA clone MGC:70871 IMAGE:6474523), complete cds.
JD527339 - Sequence 508363 from Patent EP1572962.
KJ904969 - Synthetic construct Homo sapiens clone ccsbBroadEn_14363 DNAJC24 gene, encodes complete protein.
JD061997 - Sequence 43021 from Patent EP1572962.
AL050199 - Homo sapiens mRNA; cDNA DKFZp586D0923 (from clone DKFZp586D0923).
JD566108 - Sequence 547132 from Patent EP1572962.
JD504545 - Sequence 485569 from Patent EP1572962.
JD048397 - Sequence 29421 from Patent EP1572962.
JD170467 - Sequence 151491 from Patent EP1572962.
JD245150 - Sequence 226174 from Patent EP1572962.
JD037871 - Sequence 18895 from Patent EP1572962.
JD367252 - Sequence 348276 from Patent EP1572962.
JD325953 - Sequence 306977 from Patent EP1572962.
JD153340 - Sequence 134364 from Patent EP1572962.
JD161701 - Sequence 142725 from Patent EP1572962.
JD161702 - Sequence 142726 from Patent EP1572962.
JD448617 - Sequence 429641 from Patent EP1572962.
JD078731 - Sequence 59755 from Patent EP1572962.
JD487875 - Sequence 468899 from Patent EP1572962.
JD185143 - Sequence 166167 from Patent EP1572962.
JD149240 - Sequence 130264 from Patent EP1572962.
JD514081 - Sequence 495105 from Patent EP1572962.
JD070101 - Sequence 51125 from Patent EP1572962.
JD072165 - Sequence 53189 from Patent EP1572962.
JD207531 - Sequence 188555 from Patent EP1572962.
JD242837 - Sequence 223861 from Patent EP1572962.
JD064530 - Sequence 45554 from Patent EP1572962.
JD311657 - Sequence 292681 from Patent EP1572962.
JD081483 - Sequence 62507 from Patent EP1572962.
JD356724 - Sequence 337748 from Patent EP1572962.
JD238995 - Sequence 220019 from Patent EP1572962.
JD374698 - Sequence 355722 from Patent EP1572962.
JD237805 - Sequence 218829 from Patent EP1572962.
JD184364 - Sequence 165388 from Patent EP1572962.
JD409943 - Sequence 390967 from Patent EP1572962.
JD159409 - Sequence 140433 from Patent EP1572962.
JD047501 - Sequence 28525 from Patent EP1572962.
JD432714 - Sequence 413738 from Patent EP1572962.
JD565835 - Sequence 546859 from Patent EP1572962.
JD056835 - Sequence 37859 from Patent EP1572962.
JD139095 - Sequence 120119 from Patent EP1572962.
JD286580 - Sequence 267604 from Patent EP1572962.
JD511440 - Sequence 492464 from Patent EP1572962.
JD158897 - Sequence 139921 from Patent EP1572962.
JD208685 - Sequence 189709 from Patent EP1572962.
JD443449 - Sequence 424473 from Patent EP1572962.
JD490045 - Sequence 471069 from Patent EP1572962.
JD235024 - Sequence 216048 from Patent EP1572962.
JD269686 - Sequence 250710 from Patent EP1572962.
JD367678 - Sequence 348702 from Patent EP1572962.
JD105969 - Sequence 86993 from Patent EP1572962.
JD280552 - Sequence 261576 from Patent EP1572962.
JD249081 - Sequence 230105 from Patent EP1572962.
JD535046 - Sequence 516070 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K0V0, B1ALC1, DJC24_HUMAN, DPH4, NM_181706, NP_859057, Q6P3W2, ZCSL3
UCSC ID: uc001msx.3
RefSeq Accession: NM_181706
Protein: Q6P3W2 (aka DJC24_HUMAN)
CCDS: CCDS7873.2

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_181706.4
exon count: 5CDS single in 3' UTR: no RNA size: 3000
ORF size: 450CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1084.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.