Human Gene ARL14EP (uc001mso.1)
  Description: Homo sapiens ADP-ribosylation factor-like 14 effector protein (ARL14EP), mRNA.
RefSeq Summary (NM_152316): The protein encoded by this gene is an effector protein. It interacts with ADP-ribosylation factor-like 14 [ARL14, also known as ADP-ribosylation factor 7 (ARF7)], beta-actin (ACTB) and actin-based motor protein myosin 1E (MYO1E). ARL14 is a small GTPase; it controls the export of major histocompatibility class II molecules by connecting to the actin network via this effector protein. [provided by RefSeq, Sep 2014].
Transcript (Including UTRs)
   Position: hg19 chr11:30,344,649-30,359,165 Size: 14,517 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg19 chr11:30,352,496-30,358,342 Size: 5,847 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:30,344,649-30,359,165)mRNA (may differ from genome)Protein (260 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
H-INVHGNCHPRDLynxMGIneXtProt
OMIMPubMedTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: AL14E_HUMAN
DESCRIPTION: RecName: Full=ARL14 effector protein; AltName: Full=ARF7 effector protein;
FUNCTION: Through its interaction with ARL14 and MYO1E, may connect MHC class II-containing cytoplasmic vesicles to the actin network and hence controls the movement of these vesicles along the actin cytoskeleton in dendritic cells.
SUBUNIT: Interacts with ARL14 and MYO1E.
INTERACTION: Q8N4G2:ARL14; NbExp=3; IntAct=EBI-2807994, EBI-3921493; Q12965:MYO1E; NbExp=2; IntAct=EBI-2807994, EBI-4279548;
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in the immune system.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 7.23 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 207.93 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -52.30164-0.319 Picture PostScript Text
3' UTR -226.45823-0.275 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR026515 - ARF7EP

Pfam Domains:
PF14949 - ARF7 effector protein C-terminus

ModBase Predicted Comparative 3D Structure on Q8N8R7
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserGenome BrowserNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
Gene SorterGene Sorter Gene Sorter  
 RGDEnsemblFlyBase  
 Protein SequenceProtein SequenceProtein Sequence  
 AlignmentAlignmentAlignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Cellular Component:
GO:0005634 nucleus
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0005925 focal adhesion
GO:0043231 intracellular membrane-bounded organelle


-  Descriptions from all associated GenBank mRNAs
  AK096287 - Homo sapiens cDNA FLJ38968 fis, clone NT2RI2002316.
BX647617 - Homo sapiens mRNA; cDNA DKFZp313A1137 (from clone DKFZp313A1137).
BC047775 - Homo sapiens chromosome 11 open reading frame 46, mRNA (cDNA clone MGC:54274 IMAGE:6062049), complete cds.
AK127509 - Homo sapiens cDNA FLJ45602 fis, clone BRTHA3021569.
CU690502 - Synthetic construct Homo sapiens gateway clone IMAGE:100021287 5' read C11orf46 mRNA.
KJ895352 - Synthetic construct Homo sapiens clone ccsbBroadEn_04746 C11orf46 gene, encodes complete protein.
HQ448634 - Synthetic construct Homo sapiens clone IMAGE:100072073; CCSB011576_01 chromosome 11 open reading frame 46 (C11orf46) gene, encodes complete protein.
JD027286 - Sequence 8310 from Patent EP1572962.
JD031071 - Sequence 12095 from Patent EP1572962.
JD082276 - Sequence 63300 from Patent EP1572962.
JD181940 - Sequence 162964 from Patent EP1572962.
JD374951 - Sequence 355975 from Patent EP1572962.
JD239593 - Sequence 220617 from Patent EP1572962.
JD285674 - Sequence 266698 from Patent EP1572962.
JD360550 - Sequence 341574 from Patent EP1572962.
JD037025 - Sequence 18049 from Patent EP1572962.
JD229804 - Sequence 210828 from Patent EP1572962.
JD225871 - Sequence 206895 from Patent EP1572962.
JD360833 - Sequence 341857 from Patent EP1572962.
JD332614 - Sequence 313638 from Patent EP1572962.
JD316286 - Sequence 297310 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: AL14E_HUMAN, ARF7EP, C11orf46, NM_152316, NP_689529, Q5HYH9, Q8N8R7
UCSC ID: uc001mso.1
RefSeq Accession: NM_152316
Protein: Q8N8R7 (aka AL14E_HUMAN)
CCDS: CCDS7869.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_152316.1
exon count: 4CDS single in 3' UTR: no RNA size: 1770
ORF size: 783CDS single in intron: no Alignment % ID: 99.94
txCdsPredict score: 1761.50frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.