ID:HMGC2_HUMAN DESCRIPTION: RecName: Full=3-hydroxymethyl-3-methylglutaryl-CoA lyase, cytoplasmic; EC=4.1.3.4; AltName: Full=3-hydroxymethyl-3-methylglutaryl-CoA lyase-like protein 1; AltName: Full=Endoplasmic reticulum 3-hydroxymethyl-3-methylglutaryl-CoA lyase; Short=er-cHL; FUNCTION: Non-mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy- 3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis, the products of which support energy production in nonhepatic animal tissues. CATALYTIC ACTIVITY: (S)-3-hydroxy-3-methylglutaryl-CoA = acetyl- CoA + acetoacetate. COFACTOR: Divalent metal cations (Probable). BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=40 uM for (S)-3-hydroxy-3-methylglutaryl-CoA (at pH 9) (PubMed:22847177); KM=75 uM for (S)-3-hydroxy-3-methylglutaryl-CoA (at pH 8) (PubMed:22847177); KM=28 uM for (S)-3-hydroxy-3-methylglutaryl-CoA (PubMed:22865860); KM=49 uM for (S)-3-hydroxy-3-methylglutaryl-CoA (in presence of magnesium) (PubMed:22865860); KM=0.18 uM for (S)-3-hydroxy-3-methylglutaryl-CoA (in presence of manganese) (PubMed:22865860); Vmax=12 nmol/min/mg enzyme with (S)-3-hydroxy-3-methylglutaryl- CoA as substrate at pH 9 (PubMed:22847177); Vmax=25 nmol/min/mg enzyme with (S)-3-hydroxy-3-methylglutaryl- CoA as substrate at pH 8 (PubMed:22847177); PATHWAY: Metabolic intermediate metabolism; (S)-3-hydroxy-3- methylglutaryl-CoA degradation; acetoacetate from (S)-3-hydroxy-3- methylglutaryl-CoA: step 1/1. SUBCELLULAR LOCATION: Cytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein. SIMILARITY: Belongs to the HMG-CoA lyase family. SEQUENCE CAUTION: Sequence=BAC87045.1; Type=Frameshift; Positions=253; Sequence=BAG51462.1; Type=Erroneous translation; Note=Wrong choice of CDS;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q8TB92
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
US Server
