Description: Homo sapiens ribophorin I (RPN1), mRNA. RefSeq Summary (NM_002950): This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]. Transcript (Including UTRs) Position: hg19 chr3:128,338,813-128,369,719 Size: 30,907 Total Exon Count: 10 Strand: - Coding Region Position: hg19 chr3:128,339,254-128,369,643 Size: 30,390 Coding Exon Count: 10
ID:RPN1_HUMAN DESCRIPTION: RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; EC=2.4.1.119; AltName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; AltName: Full=Ribophorin I; Short=RPN-I; AltName: Full=Ribophorin-1; Flags: Precursor; FUNCTION: Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. CATALYTIC ACTIVITY: Dolichyl diphosphooligosaccharide + protein L- asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L- asparagine. SUBUNIT: Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. TISSUE SPECIFICITY: Expressed in all tissues tested. SIMILARITY: Belongs to the OST1 family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P04843
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006464 cellular protein modification process GO:0006486 protein glycosylation GO:0018279 protein N-linked glycosylation via asparagine
BC010839 - Homo sapiens ribophorin I, mRNA (cDNA clone MGC:5072 IMAGE:3461167), complete cds. CR749284 - Homo sapiens mRNA; cDNA DKFZp686B16177 (from clone DKFZp686B16177). AK223595 - Homo sapiens mRNA for ribophorin I variant, clone: FCC131A08. Y00281 - Human mRNA for ribophorin I. BC007995 - Homo sapiens, Similar to ribophorin I, clone IMAGE:3609303, mRNA, partial cds. AK297513 - Homo sapiens cDNA FLJ50809 complete cds, highly similar to Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit precursor (EC 2.4.1.119). JD118923 - Sequence 99947 from Patent EP1572962. JD347126 - Sequence 328150 from Patent EP1572962. JD273245 - Sequence 254269 from Patent EP1572962. JD230653 - Sequence 211677 from Patent EP1572962. AK297941 - Homo sapiens cDNA FLJ51908 complete cds, highly similar to Dolichyl-diphosphooligosaccharide--proteinglycosyltransferase 67 kDa subunit precursor (EC 2.4.1.119). AK296905 - Homo sapiens cDNA FLJ51740 complete cds, highly similar to Dolichyl-diphosphooligosaccharide--proteinglycosyltransferase 67 kDa subunit precursor (EC 2.4.1.119). JD270896 - Sequence 251920 from Patent EP1572962. AK312369 - Homo sapiens cDNA, FLJ92691, Homo sapiens ribophorin I (RPN1), mRNA. JF432331 - Synthetic construct Homo sapiens clone IMAGE:100073518 ribophorin I (RPN1) gene, encodes complete protein. KJ897494 - Synthetic construct Homo sapiens clone ccsbBroadEn_06888 RPN1 gene, encodes complete protein. AB527671 - Synthetic construct DNA, clone: pF1KB6179, Homo sapiens RPN1 gene for ribophorin I, without stop codon, in Flexi system. CR456742 - Homo sapiens full open reading frame cDNA clone RZPDo834H0114D for gene RPN1, ribophorin I; complete cds, incl. stopcodon. DQ573369 - Homo sapiens piRNA piR-41481, complete sequence. CU679817 - Synthetic construct Homo sapiens gateway clone IMAGE:100020403 5' read RPN1 mRNA. AF310158 - Homo sapiens ribophorin I/EVI-1 chimeric fusion protein variant (RPN1/EVI-1 fusion) mRNA, partial cds.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa00510 - N-Glycan biosynthesis hsa01100 - Metabolic pathways
Reactome (by CSHL, EBI, and GO)
Protein P04843 (Reactome details) participates in the following event(s):
R-HSA-1799326 Signal-containing nascent peptide translocates to endoplasmic reticulum R-HSA-1799329 Signal peptidase hydrolyzes signal peptide from ribosome-associated nascent protein R-HSA-446209 Transfer of N-glycan to the protein R-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane R-HSA-72766 Translation R-HSA-446203 Asparagine N-linked glycosylation R-HSA-392499 Metabolism of proteins R-HSA-597592 Post-translational protein modification
US Server
