Human Gene GK2 (uc003hlu.3)
  Description: Homo sapiens glycerol kinase 2 (GK2), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr4:80,327,507-80,329,372 Size: 1,866 Total Exon Count: 1 Strand: -
Coding Region
   Position: hg19 chr4:80,327,693-80,329,354 Size: 1,662 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsMalaCards
CTDGene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein Structure
Other SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther NamesModel Information
Methods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:80,327,507-80,329,372)mRNA (may differ from genome)Protein (553 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMalacards
MGIneXtProtOMIMPubMedReactomeTreefam
UniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GLPK2_HUMAN
DESCRIPTION: RecName: Full=Glycerol kinase 2; Short=GK 2; Short=Glycerokinase 2; EC=2.7.1.30; AltName: Full=ATP:glycerol 3-phosphotransferase 2; AltName: Full=Glycerol kinase, testis specific 2;
FUNCTION: Key enzyme in the regulation of glycerol uptake and metabolism (By similarity).
CATALYTIC ACTIVITY: ATP + glycerol = ADP + sn-glycerol 3- phosphate.
PATHWAY: Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
SUBCELLULAR LOCATION: Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasm (By similarity). Note=In sperm the majority of the enzyme is bound to mitochondria (By similarity).
SIMILARITY: Belongs to the FGGY kinase family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): GK2
CDC HuGE Published Literature: GK2

-  MalaCards Disease Associations
  MalaCards Gene Search: GK2
Diseases sorted by gene-association score: glycerol kinase deficiency (9)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 71.29 RPKM in Testis
Total median expression: 71.40 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -2.8018-0.156 Picture PostScript Text
3' UTR -28.61186-0.154 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR018485 - Carb_kinase_FGGY_C
IPR018483 - Carb_kinase_FGGY_CS
IPR018484 - Carb_kinase_FGGY_N
IPR005999 - Glycerol_kin

Pfam Domains:
PF00370 - FGGY family of carbohydrate kinases, N-terminal domain
PF02782 - FGGY family of carbohydrate kinases, C-terminal domain

SCOP Domains:
53067 - Actin-like ATPase domain

ModBase Predicted Comparative 3D Structure on Q14410
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologGenome Browser
     Gene Details
     Gene Sorter
     SGD
     Protein Sequence
     Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004370 glycerol kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0016773 phosphotransferase activity, alcohol group as acceptor

Biological Process:
GO:0005975 carbohydrate metabolic process
GO:0006071 glycerol metabolic process
GO:0006072 glycerol-3-phosphate metabolic process
GO:0006641 triglyceride metabolic process
GO:0016310 phosphorylation
GO:0019563 glycerol catabolic process
GO:0046167 glycerol-3-phosphate biosynthetic process

Cellular Component:
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005741 mitochondrial outer membrane
GO:0016020 membrane
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AK292241 - Homo sapiens cDNA FLJ75853 complete cds, highly similar to Homo sapiens glycerol kinase 2 (GK2), mRNA.
X78712 - H.sapiens mRNA for glycerol kinase testis specific 2.
HM005700 - Homo sapiens clone HTL-T-77m testis tissue sperm-binding protein Li 77m mRNA, complete cds.
BC058888 - Homo sapiens glycerol kinase 2, mRNA (cDNA clone MGC:64903 IMAGE:5172337), complete cds.
BC029820 - Homo sapiens glycerol kinase 2, mRNA (cDNA clone MGC:35147 IMAGE:5169374), complete cds.
BC027598 - Homo sapiens cDNA clone IMAGE:4827415, containing frame-shift errors.
BC048274 - Homo sapiens glycerol kinase 2, mRNA (cDNA clone MGC:51785 IMAGE:5166566), complete cds.
AK312768 - Homo sapiens cDNA, FLJ93182.
DQ891321 - Synthetic construct clone IMAGE:100003951; FLH171069.01X; RZPDo839E09100D glycerol kinase 2 (GK2) gene, encodes complete protein.
KJ896885 - Synthetic construct Homo sapiens clone ccsbBroadEn_06279 GK2 gene, encodes complete protein.
KJ905204 - Synthetic construct Homo sapiens clone ccsbBroadEn_14656 GK2 gene, encodes complete protein.
DQ894505 - Synthetic construct Homo sapiens clone IMAGE:100008965; FLH171065.01L; RZPDo839E0999D glycerol kinase 2 (GK2) gene, encodes complete protein.
CU688758 - Synthetic construct Homo sapiens gateway clone IMAGE:100019849 5' read GK2 mRNA.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00561 - Glycerolipid metabolism
hsa01100 - Metabolic pathways
hsa03320 - PPAR signaling pathway

BioCyc Knowledge Library
PWY-4261 - glycerol degradation IV

Reactome (by CSHL, EBI, and GO)

Protein Q14410 (Reactome details) participates in the following event(s):

R-HSA-75887 Conversion of Glycerol to Glycerol-3-phosphate
R-HSA-75109 Triglyceride biosynthesis
R-HSA-8979227 Triglyceride metabolism
R-HSA-556833 Metabolism of lipids
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: A8K876, GKP2, GKTA, GLPK2_HUMAN, NM_033214, NP_149991, Q14410, Q6PD73, Q86XV8
UCSC ID: uc003hlu.3
RefSeq Accession: NM_033214
Protein: Q14410 (aka GLPK2_HUMAN)
CCDS: CCDS3585.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_033214.2
exon count: 1CDS single in 3' UTR: no RNA size: 1899
ORF size: 1662CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3524.00frame shift in genome: no % Coverage: 98.26
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.