Description: Homo sapiens cleavage and polyadenylation specific factor 1, 160kDa (CPSF1), mRNA. RefSeq Summary (NM_013291): Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).[supplied by OMIM, Mar 2008]. Transcript (Including UTRs) Position: hg19 chr8:145,618,446-145,634,733 Size: 16,288 Total Exon Count: 38 Strand: - Coding Region Position: hg19 chr8:145,618,533-145,634,542 Size: 16,010 Coding Exon Count: 37
ID:CPSF1_HUMAN DESCRIPTION: RecName: Full=Cleavage and polyadenylation specificity factor subunit 1; AltName: Full=Cleavage and polyadenylation specificity factor 160 kDa subunit; Short=CPSF 160 kDa subunit; FUNCTION: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre- mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction. SUBUNIT: Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. Found in a complex with CPSF1, FIP1L1 and PAPOLA. Interacts with FIP1L1, PAPD4/GLD2 and SRRM1. Interacts with TUT1; the interaction is direct and mediates the recruitment of the CPSF complex on the 3'UTR of selected pre-mRNAs. SUBCELLULAR LOCATION: Nucleus, nucleoplasm. PTM: The N-terminus is blocked. SIMILARITY: Belongs to the CPSF1 family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF03178 - CPSF A subunit region PF10433 - Mono-functional DNA-alkylating methyl methanesulfonate N-term
ModBase Predicted Comparative 3D Structure on Q10570
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
BC009954 - Homo sapiens cleavage and polyadenylation specific factor 1, 160kDa, mRNA (cDNA clone IMAGE:4130156), partial cds. BC017232 - Homo sapiens cleavage and polyadenylation specific factor 1, 160kDa, mRNA (cDNA clone MGC:15424 IMAGE:4300196), complete cds. U37012 - Human cleavage and polyadenylation specificity factor mRNA, complete cds. DQ893479 - Synthetic construct clone IMAGE:100006109; FLH193598.01X; RZPDo839H1178D cleavage and polyadenylation specific factor 1, 160kDa (CPSF1) gene, encodes complete protein. DQ896356 - Synthetic construct Homo sapiens clone IMAGE:100010816; FLH193594.01L; RZPDo839H1168D cleavage and polyadenylation specific factor 1, 160kDa (CPSF1) gene, encodes complete protein. AB590264 - Synthetic construct DNA, clone: pFN21AE1516, Homo sapiens CPSF1 gene for cleavage and polyadenylation specific factor 1, 160kDa, without stop codon, in Flexi system. AK293601 - Homo sapiens cDNA FLJ60434 complete cds, highly similar to Cleavage and polyadenylation specificity factor 160 kDa subunit. AB046744 - Homo sapiens P/cl.18 mRNA for polyadenylation specificity factor, complete cds. BC028197 - Homo sapiens, clone IMAGE:5211820, mRNA. CU677871 - Synthetic construct Homo sapiens gateway clone IMAGE:100023486 5' read CPSF1 mRNA.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_cpsfPathway - Polyadenylation of mRNA
Reactome (by CSHL, EBI, and GO)
Protein Q10570 (Reactome details) participates in the following event(s):
R-HSA-72231 Cleavage and Polyadenylation R-HSA-77589 Recognition of AAUAAA sequence by CPSF R-HSA-72185 mRNA polyadenylation R-HSA-77594 Docking of the Mature intronless derived transcript derived mRNA, TAP and Aly/Ref at the NPC R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-72180 Cleavage of mRNA at the 3'-end R-HSA-77590 Recruitment of CstF to the CPSF Bound Pre-mRNA R-HSA-77592 Cleavage of Intronless Pre-mRNA at 3'-end R-HSA-77593 Cleavage and polyadenylation of Intronless Pre-mRNA R-HSA-5696813 TSEN complex cleaves the intron from pre-tRNA R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-8849157 TREX complex binds spliced, capped mRNA:CBC:EJC cotranscriptionally R-HSA-156661 Formation of Exon Junction Complex R-HSA-77591 Binding of Cleavage factors and Poly(A)Polymerase to the CstF:CPSF:Pre-mRNA Complex R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-77595 Processing of Intronless Pre-mRNAs R-HSA-72187 mRNA 3'-end processing R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-109688 Cleavage of Growing Transcript in the Termination Region R-HSA-72172 mRNA Splicing R-HSA-75067 Processing of Capped Intronless Pre-mRNA R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-159234 Transport of Mature mRNAs Derived from Intronless Transcripts R-HSA-6784531 tRNA processing in the nucleus R-HSA-73856 RNA Polymerase II Transcription Termination R-HSA-8953854 Metabolism of RNA R-HSA-72202 Transport of Mature Transcript to Cytoplasm R-HSA-72306 tRNA processing R-HSA-73857 RNA Polymerase II Transcription R-HSA-74160 Gene expression (Transcription)
US Server
