Human Gene TOE1 (uc009vxq.3)
  Description: Homo sapiens target of EGR1, member 1 (nuclear) (TOE1), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr1:45,805,342-45,809,650 Size: 4,309 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg19 chr1:45,805,925-45,809,374 Size: 3,450 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:45,805,342-45,809,650)mRNA (may differ from genome)Protein (510 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMalacards
MGIneXtProtOMIMPubMedTreefamUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TOE1_HUMAN
DESCRIPTION: RecName: Full=Target of EGR1 protein 1;
FUNCTION: Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1.
SUBCELLULAR LOCATION: Nucleus, nucleolus. Nucleus speckle. Note=Localizes to nuclear speckles.
SIMILARITY: Belongs to the CAF1 family.
SIMILARITY: Contains 1 C3H1-type zinc finger.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TOE1
Diseases sorted by gene-association score: pontocerebellar hypoplasia, type 7* (1569), pontocerebellar hypoplasia (17)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 14.32 RPKM in Testis
Total median expression: 241.77 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -228.20583-0.391 Picture PostScript Text
3' UTR -73.80276-0.267 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR006941 - RNase_CAF1
IPR012337 - RNaseH-like_dom
IPR000571 - Znf_CCCH

Pfam Domains:
PF00642 - Zinc finger C-x8-C-x5-C-x3-H type (and similar)
PF04857 - CAF1 family ribonuclease

SCOP Domains:
49417 - p53-like transcription factors
53098 - Ribonuclease H-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2FC6 - NMR MuPIT


ModBase Predicted Comparative 3D Structure on Q96GM8
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserNo orthologGenome BrowserNo ortholog
Gene Details   Gene Details 
Gene Sorter   Gene Sorter 
  Ensembl WormBase 
  Protein Sequence Protein Sequence 
  Alignment Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000175 3'-5'-exoribonuclease activity
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003676 nucleic acid binding
GO:0004535 poly(A)-specific ribonuclease activity
GO:0005515 protein binding
GO:0017069 snRNA binding
GO:0046872 metal ion binding

Biological Process:
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0034472 snRNA 3'-end processing
GO:0090503 RNA phosphodiester bond hydrolysis, exonucleolytic

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0015030 Cajal body
GO:0016604 nuclear body
GO:0016607 nuclear speck


-  Descriptions from all associated GenBank mRNAs
  AY169960 - Homo sapiens target of Egr1 protein (TOE1) mRNA, complete cds.
JD429110 - Sequence 410134 from Patent EP1572962.
JD425521 - Sequence 406545 from Patent EP1572962.
JD532664 - Sequence 513688 from Patent EP1572962.
JD439814 - Sequence 420838 from Patent EP1572962.
JD227999 - Sequence 209023 from Patent EP1572962.
JD379319 - Sequence 360343 from Patent EP1572962.
JD202088 - Sequence 183112 from Patent EP1572962.
AK293704 - Homo sapiens cDNA FLJ59313 complete cds, highly similar to Homo sapiens target of EGR1, member 1 (nuclear) (TOE1), mRNA.
AK024011 - Homo sapiens cDNA FLJ13949 fis, clone Y79AA1001041.
AK298162 - Homo sapiens cDNA FLJ57546 complete cds, highly similar to Homo sapiens target of EGR1, member 1 (nuclear) (TOE1), mRNA.
AK093320 - Homo sapiens cDNA FLJ36001 fis, clone TESTI2015213, highly similar to Homo sapiens target of EGR1, member 1 (nuclear) (TOE1), mRNA.
BC009364 - Homo sapiens target of EGR1, member 1 (nuclear), mRNA (cDNA clone MGC:14971 IMAGE:4302712), complete cds.
JF432181 - Synthetic construct Homo sapiens clone IMAGE:100073336 target of EGR1, member 1 (nuclear) (TOE1) gene, encodes complete protein.
CU679325 - Synthetic construct Homo sapiens gateway clone IMAGE:100019735 5' read TOE1 mRNA.
CR457320 - Homo sapiens full open reading frame cDNA clone RZPDo834F0313D for gene TOE1, target of EGR1, member 1 (nuclear); complete cds, incl. stopcodon.
DQ585191 - Homo sapiens piRNA piR-52303, complete sequence.

-  Other Names for This Gene
  Alternate Gene Symbols: NM_025077, NP_079353, Q6IA35, Q8IWN5, Q96GM8, Q9H846, TOE1_HUMAN
UCSC ID: uc009vxq.3
RefSeq Accession: NM_025077
Protein: Q96GM8 (aka TOE1_HUMAN)
CCDS: CCDS521.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_025077.3
exon count: 8CDS single in 3' UTR: no RNA size: 2408
ORF size: 1533CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3158.00frame shift in genome: no % Coverage: 99.34
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.