Description: Homo sapiens leucine carboxyl methyltransferase 2 (LCMT2), mRNA. RefSeq Summary (NM_014793): The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). [provided by RefSeq, Jul 2008]. Transcript (Including UTRs) Position: hg19 chr15:43,619,974-43,622,820 Size: 2,847 Total Exon Count: 1 Strand: - Coding Region Position: hg19 chr15:43,620,627-43,622,687 Size: 2,061 Coding Exon Count: 1
ID:LCMT2_HUMAN DESCRIPTION: RecName: Full=Leucine carboxyl methyltransferase 2; EC=2.1.1.-; AltName: Full=p21WAF1/CIP1 promoter-interacting protein; AltName: Full=tRNA wybutosine-synthesizing protein 4 homolog; Short=tRNA yW-synthesizing protein 4 homolog; FUNCTION: Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha- leucine ester residues. PATHWAY: tRNA modification; wybutosine-tRNA(Phe) biosynthesis. SUBUNIT: Interacts with RNF144B/IBRDC2. SIMILARITY: Belongs to the methyltransferase superfamily. LCMT family. SEQUENCE CAUTION: Sequence=BAA25473.2; Type=Erroneous initiation; WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/lcmt2/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O60294
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Protein O60294 (Reactome details) participates in the following event(s):
R-HSA-6782881 LCMT2 methylates yW-72 yielding yW-58 at nucleotide 37 of tRNA(Phe) R-HSA-6782890 LCMT2 methoxycarbonylates yW-58 yielding yW (wybutosine) at nucleotide 37 of tRNA(Phe) R-HSA-6783473 LCMT2 methoxycarbonylates OHyW-72 yielding OHyW (hydroxywybutosine) at nucleotide 37 of tRNA(Phe) R-HSA-6782861 Synthesis of wybutosine at G37 of tRNA(Phe) R-HSA-6782315 tRNA modification in the nucleus and cytosol R-HSA-72306 tRNA processing R-HSA-8953854 Metabolism of RNA