Human Gene METTL3 (uc001wbc.3) Description and Page Index
  Description: Homo sapiens methyltransferase like 3 (METTL3), mRNA.
RefSeq Summary (NM_019852): This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.114137.1, SRR1163655.125585.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000298717.9/ ENSP00000298717.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##
Transcript (Including UTRs)
   Position: hg19 chr14:21,966,282-21,979,457 Size: 13,176 Total Exon Count: 11 Strand: -
Coding Region
   Position: hg19 chr14:21,966,402-21,979,365 Size: 12,964 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr14:21,966,282-21,979,457)mRNA (may differ from genome)Protein (580 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
neXtProtOMIMPubMedReactomeStanford SOURCETreefam

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=N6-adenosine-methyltransferase 70 kDa subunit; Short=MT-A70; EC=; AltName: Full=Methyltransferase-like protein 3;
FUNCTION: N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + m(7)G(5')pppAm = S- adenosyl-L-homocysteine + m(7)G(5')pppm(6)Am.
SUBCELLULAR LOCATION: Nucleus speckle. Note=Colocalizes with speckles in interphase nuclei. Suggesting that it may be associated with nuclear pre-mRNA splicing components.
TISSUE SPECIFICITY: Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the MT-A70-like family.

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): METTL3
CDC HuGE Published Literature: METTL3
Positive Disease Associations: Uric Acid
Related Studies:
  1. Uric Acid
    Shih-Jen Hwang et al. BMC medical genetics 2007, A genome-wide association for kidney function and endocrine-related traits in the NHLBI's Framingham Heart Study., BMC medical genetics. [PubMed 17903292]
    Kidney function traits and TSH are associated with SNPs on the Affymetrix GeneChip Human Mapping 100K SNP set. These data will serve as a valuable resource for replication as more SNPs associated with kidney function and endocrine traits are identified.

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 29.56 RPKM in Thyroid
Total median expression: 880.52 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -31.6092-0.343 Picture PostScript Text
3' UTR -24.20120-0.202 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR025848 - MT-A70
IPR007757 - MT-A70-like

Pfam Domains:
PF05063 - MT-A70

SCOP Domains:
53335 - S-adenosyl-L-methionine-dependent methyltransferases

ModBase Predicted Comparative 3D Structure on Q86U44
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserGenome BrowserNo orthologGenome Browser
Gene DetailsGene Details Gene Details Gene Details
Gene SorterGene Sorter Gene Sorter Gene Sorter
 RGDEnsemblFlyBase SGD
 Protein SequenceProtein SequenceProtein Sequence Protein Sequence
 AlignmentAlignmentAlignment Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0005515 protein binding
GO:0008168 methyltransferase activity
GO:0008173 RNA methyltransferase activity
GO:0016422 mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity
GO:0016740 transferase activity
GO:0046982 protein heterodimerization activity
GO:1904047 S-adenosyl-L-methionine binding

Biological Process:
GO:0000398 mRNA splicing, via spliceosome
GO:0001510 RNA methylation
GO:0006382 adenosine to inosine editing
GO:0006397 mRNA processing
GO:0006402 mRNA catabolic process
GO:0006974 cellular response to DNA damage stimulus
GO:0007283 spermatogenesis
GO:0007623 circadian rhythm
GO:0019827 stem cell population maintenance
GO:0021861 forebrain radial glial cell differentiation
GO:0030154 cell differentiation
GO:0031053 primary miRNA processing
GO:0032259 methylation
GO:0034644 cellular response to UV
GO:0042063 gliogenesis
GO:0045580 regulation of T cell differentiation
GO:0045746 negative regulation of Notch signaling pathway
GO:0048511 rhythmic process
GO:0051445 regulation of meiotic cell cycle
GO:0061157 mRNA destabilization
GO:0080009 mRNA methylation
GO:0098508 endothelial to hematopoietic transition
GO:1902036 regulation of hematopoietic stem cell differentiation
GO:1903679 positive regulation of cap-independent translational initiation
GO:1990744 primary miRNA methylation

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0016607 nuclear speck
GO:0036396 MIS complex

-  Descriptions from all associated GenBank mRNAs
  LF210056 - JP 2014500723-A/17559: Polycomb-Associated Non-Coding RNAs.
AK098655 - Homo sapiens cDNA FLJ25789 fis, clone TST06885, highly similar to Homo sapiens (N6-adenosine)-methyltransferase gene.
BC052244 - Homo sapiens methyltransferase like 3, mRNA (cDNA clone MGC:59649 IMAGE:6081642), complete cds.
BC014427 - Homo sapiens cDNA clone IMAGE:4564560, containing frame-shift errors.
AK124183 - Homo sapiens cDNA FLJ42189 fis, clone THYMU2032601.
AK000222 - Homo sapiens cDNA FLJ20215 fis, clone COLF2016.
BC001650 - Homo sapiens methyltransferase like 3, mRNA (cDNA clone MGC:2708 IMAGE:2821235), complete cds.
BC003031 - Homo sapiens methyltransferase like 3, mRNA (cDNA clone MGC:4336 IMAGE:2821235), complete cds.
AK093509 - Homo sapiens cDNA FLJ36190 fis, clone TESTI2027271, highly similar to N6-adenosine-methyltransferase 70 kDa subunit (EC
AK097873 - Homo sapiens cDNA FLJ40554 fis, clone THYMU2002338, moderately similar to Homo sapiens m6A methyltransferase (MT-A70) gene.
BX247964 - human full-length cDNA clone CS0DE001YM14 of Placenta of Homo sapiens (human).
EU446825 - Synthetic construct Homo sapiens clone IMAGE:100069902; IMAGE:100012034; FLH257065.01L methyltransferase like 3 (METTL3) gene, encodes complete protein.
LF334383 - JP 2014500723-A/141886: Polycomb-Associated Non-Coding RNAs.
LF334384 - JP 2014500723-A/141887: Polycomb-Associated Non-Coding RNAs.
AK304570 - Homo sapiens cDNA FLJ57780 complete cds, highly similar to N6-adenosine-methyltransferase 70 kDa subunit (EC
AK304251 - Homo sapiens cDNA FLJ61069 complete cds, highly similar to N6-adenosine-methyltransferase 70 kDa subunit(EC
AK300289 - Homo sapiens cDNA FLJ58857 complete cds, highly similar to N6-adenosine-methyltransferase 70 kDa subunit (EC
DQ581770 - Homo sapiens piRNA piR-49882, complete sequence.
JD108845 - Sequence 89869 from Patent EP1572962.
JD232389 - Sequence 213413 from Patent EP1572962.
MA445633 - JP 2018138019-A/17559: Polycomb-Associated Non-Coding RNAs.
MA569960 - JP 2018138019-A/141886: Polycomb-Associated Non-Coding RNAs.
MA569961 - JP 2018138019-A/141887: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q86U44 (Reactome details) participates in the following event(s):

R-HSA-72095 Internal Methylation of mRNA
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-8953854 Metabolism of RNA

-  Other Names for This Gene
  Alternate Gene Symbols: MTA70, MTA70_HUMAN, NM_019852, NP_062826, O14736, Q86U44, Q86V05, Q9HB32
UCSC ID: uc001wbc.3
RefSeq Accession: NM_019852
Protein: Q86U44 (aka MTA70_HUMAN or MT70_HUMAN)
CCDS: CCDS32044.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: NM_019852.3
exon count: 11CDS single in 3' UTR: no RNA size: 1975
ORF size: 1743CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3686.00frame shift in genome: no % Coverage: 98.99
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: yes # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.