Description: Homo sapiens alkB, alkylation repair homolog 3 (E. coli) (ALKBH3), mRNA. RefSeq Summary (NM_139178): The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]. Transcript (Including UTRs) Position: hg19 chr11:43,902,357-43,941,825 Size: 39,469 Total Exon Count: 10 Strand: + Coding Region Position: hg19 chr11:43,904,203-43,941,560 Size: 37,358 Coding Exon Count: 9
ID:ALKB3_HUMAN DESCRIPTION: RecName: Full=Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; EC=1.14.11.-; AltName: Full=Alkylated DNA repair protein alkB homolog 3; AltName: Full=DEPC-1; AltName: Full=Prostate cancer antigen 1; FUNCTION: Dioxygenase that repairs alkylated DNA containing 1- methyladenine (1meA) and 3-methylcytosine (3meC) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated 3mC within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron. COFACTOR: Binds 1 Fe(2+) ion per subunit. ENZYME REGULATION: Activated by ascorbate. SUBUNIT: Interacts with the TRIP4 complex, notably composed of ASCC3. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. TISSUE SPECIFICITY: Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon. SIMILARITY: Belongs to the alkB family. SIMILARITY: Contains 1 Fe2OG dioxygenase domain. SEQUENCE CAUTION: Sequence=AAH15155.1; Type=Erroneous initiation; WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/depc1/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q96Q83
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006281 DNA repair GO:0006307 DNA dealkylation involved in DNA repair GO:0006974 cellular response to DNA damage stimulus GO:0008283 cell proliferation GO:0035552 oxidative single-stranded DNA demethylation GO:0035553 oxidative single-stranded RNA demethylation GO:0055114 oxidation-reduction process
LF205787 - JP 2014500723-A/13290: Polycomb-Associated Non-Coding RNAs. AK315266 - Homo sapiens cDNA, FLJ96275. AB042029 - Homo sapiens DEPC-1 mRNA for prostate cancer antigen-1, complete cds. BC067257 - Homo sapiens alkB, alkylation repair homolog 3 (E. coli), mRNA (cDNA clone MGC:74669 IMAGE:6176933), complete cds. BC103812 - Homo sapiens alkB, alkylation repair homolog 3 (E. coli), mRNA (cDNA clone MGC:118790 IMAGE:40000372), complete cds. BC103813 - Homo sapiens alkB, alkylation repair homolog 3 (E. coli), mRNA (cDNA clone MGC:118792 IMAGE:40000375), complete cds. BC103814 - Homo sapiens alkB, alkylation repair homolog 3 (E. coli), mRNA (cDNA clone MGC:118793 IMAGE:40000376), complete cds. KJ900472 - Synthetic construct Homo sapiens clone ccsbBroadEn_09866 ALKBH3 gene, encodes complete protein. KJ904026 - Synthetic construct Homo sapiens clone ccsbBroadEn_13420 ALKBH3 gene, encodes complete protein. MA441364 - JP 2018138019-A/13290: Polycomb-Associated Non-Coding RNAs. JD132079 - Sequence 113103 from Patent EP1572962. JD202602 - Sequence 183626 from Patent EP1572962. JD085489 - Sequence 66513 from Patent EP1572962. LF348158 - JP 2014500723-A/155661: Polycomb-Associated Non-Coding RNAs. MA583735 - JP 2018138019-A/155661: Polycomb-Associated Non-Coding RNAs. BC015155 - Homo sapiens alkB, alkylation repair homolog 3 (E. coli), mRNA (cDNA clone IMAGE:3883498), complete cds. KJ906459 - Synthetic construct Homo sapiens clone ccsbBroadEn_16129 ALKBH3 gene, encodes complete protein. AK125602 - Homo sapiens cDNA FLJ43614 fis, clone SPLEN2016053. LF348140 - JP 2014500723-A/155643: Polycomb-Associated Non-Coding RNAs. JD307292 - Sequence 288316 from Patent EP1572962. JD516631 - Sequence 497655 from Patent EP1572962. JD381787 - Sequence 362811 from Patent EP1572962. U38442 - Human clone JkA1 mRNA induced upon T-cell activation, 3' end. JD214512 - Sequence 195536 from Patent EP1572962. JD487963 - Sequence 468987 from Patent EP1572962. JD228051 - Sequence 209075 from Patent EP1572962. JD168852 - Sequence 149876 from Patent EP1572962. MA583717 - JP 2018138019-A/155643: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q96Q83 (Reactome details) participates in the following event(s):
R-HSA-5657617 ALKBH3 associated with ASCC1:ASCC2:ASCC3 binds alkylated dsDNA containing 3-meC R-HSA-5657637 ALKBH3 associated with ASCC1:ASCC2:ASCC3 binds alkylated dsDNA containing 1-meA R-HSA-5657642 ALKBH3 in complex with ASCC1:ASCC2:ASCC3 binds alkylated DNA containing 1-etA R-HSA-112123 Oxidative demethylation of 1-meA damaged DNA By ALKBH3 R-HSA-112124 Oxidative demethylation of 3-meC damaged DNA By ALKBH3 R-HSA-112125 Oxidative dealkylation of 1-EtA damaged DNA by ABH3 R-HSA-112126 ALKBH3 mediated reversal of alkylation damage R-HSA-73943 Reversal of alkylation damage by DNA dioxygenases R-HSA-73942 DNA Damage Reversal R-HSA-73894 DNA Repair