Description: Homo sapiens ubiquitin specific peptidase 16 (USP16), transcript variant 1, mRNA. RefSeq Summary (NM_006447): This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]. Transcript (Including UTRs) Position: hg19 chr21:30,396,938-30,426,807 Size: 29,870 Total Exon Count: 18 Strand: + Coding Region Position: hg19 chr21:30,400,235-30,426,508 Size: 26,274 Coding Exon Count: 17
ID:UBP16_HUMAN DESCRIPTION: RecName: Full=Ubiquitin carboxyl-terminal hydrolase 16; EC=3.4.19.12; AltName: Full=Deubiquitinating enzyme 16; AltName: Full=Ubiquitin thioesterase 16; AltName: Full=Ubiquitin-processing protease UBP-M; AltName: Full=Ubiquitin-specific-processing protease 16; FUNCTION: Specifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-10' of histone H3, and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. CATALYTIC ACTIVITY: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C- terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Nucleus (Probable). TISSUE SPECIFICITY: Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. DOMAIN: The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin. PTM: Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. The phosphorylated form of the protein is also enzymatically active. DISEASE: Note=A chromosomal aberration involving USP16 is a cause of Chronic myelomonocytic leukemia. Inversion inv(21) (q21;q22) with RUNX1/AML1. SIMILARITY: Belongs to the peptidase C19 family. USP16 subfamily. SIMILARITY: Contains 1 UBP-type zinc finger. SEQUENCE CAUTION: Sequence=AAG39290.1; Type=Erroneous initiation; Sequence=BAG51175.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF00443 - Ubiquitin carboxyl-terminal hydrolase PF02148 - Zn-finger in ubiquitin-hydrolases and other protein PF13423 - Ubiquitin carboxyl-terminal hydrolase
ModBase Predicted Comparative 3D Structure on Q9Y5T5
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
US Server
