Human Gene MED10 (uc003jdo.3) Description and Page Index
  Description: Homo sapiens mediator complex subunit 10 (MED10), mRNA.
RefSeq Summary (NM_032286): MED10 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM, Oct 2008]. ##Evidence-Data-START## Transcript exon combination :: CB149061.1, SRR5189652.30109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467146 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000255764.4/ ENSP00000255764.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##
Transcript (Including UTRs)
   Position: hg19 chr5:6,372,039-6,378,639 Size: 6,601 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg19 chr5:6,372,616-6,378,596 Size: 5,981 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:6,372,039-6,378,639)mRNA (may differ from genome)Protein (135 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMGI
neXtProtOMIMPubMedReactomeStanford SOURCETreefam
UniProtKB

-  Comments and Description Text from UniProtKB
  ID: MED10_HUMAN
DESCRIPTION: RecName: Full=Mediator of RNA polymerase II transcription subunit 10; AltName: Full=Mediator complex subunit 10; AltName: Full=Transformation-related gene 17 protein; Short=TRG-17; AltName: Full=Transformation-related gene 20 protein; Short=TRG-20;
FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
SUBUNIT: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
SUBCELLULAR LOCATION: Nucleus (Probable).
SIMILARITY: Belongs to the Mediator complex subunit 10 family.

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): MED10
CDC HuGE Published Literature: MED10
Positive Disease Associations: Iron
Related Studies:
  1. Iron
    , , . [PubMed 0]

-  MalaCards Disease Associations
  MalaCards Gene Search: MED10
Diseases sorted by gene-association score: cri-du-chat syndrome (4)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 30.63 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 980.38 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -14.9043-0.347 Picture PostScript Text
3' UTR -186.85577-0.324 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR019145 - Mediator_Med10

Pfam Domains:
PF09748 - Transcription factor subunit Med10 of Mediator complex

ModBase Predicted Comparative 3D Structure on Q9BTT4
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserGenome BrowserGenome Browser
Gene Details  Gene DetailsGene DetailsGene Details
Gene Sorter  Gene SorterGene SorterGene Sorter
  EnsemblFlyBaseWormBaseSGD
  Protein SequenceProtein SequenceProtein SequenceProtein Sequence
  AlignmentAlignmentAlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001104 RNA polymerase II transcription cofactor activity
GO:0005515 protein binding
GO:0061630 ubiquitin protein ligase activity

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0016567 protein ubiquitination
GO:0019827 stem cell population maintenance
GO:0045944 positive regulation of transcription from RNA polymerase II promoter

Cellular Component:
GO:0000151 ubiquitin ligase complex
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0016592 mediator complex
GO:0070847 core mediator complex


-  Descriptions from all associated GenBank mRNAs
  AK075493 - Homo sapiens cDNA PSEC0190 fis, clone HEMBA1000296.
JD229780 - Sequence 210804 from Patent EP1572962.
JD263035 - Sequence 244059 from Patent EP1572962.
BC003353 - Homo sapiens mediator complex subunit 10, mRNA (cDNA clone MGC:5309 IMAGE:2899986), complete cds.
JD259368 - Sequence 240392 from Patent EP1572962.
JD370593 - Sequence 351617 from Patent EP1572962.
JD282096 - Sequence 263120 from Patent EP1572962.
JD467270 - Sequence 448294 from Patent EP1572962.
JD072670 - Sequence 53694 from Patent EP1572962.
JD355851 - Sequence 336875 from Patent EP1572962.
JD498171 - Sequence 479195 from Patent EP1572962.
JD199660 - Sequence 180684 from Patent EP1572962.
AY277599 - Homo sapiens transformation-related protein 17 (TRG17) mRNA, complete cds.
KJ894951 - Synthetic construct Homo sapiens clone ccsbBroadEn_04345 MED10 gene, encodes complete protein.
AY598325 - Homo sapiens L6 mRNA, complete cds.
AY453397 - Homo sapiens transformation-related protein 20 (TRG20) mRNA, complete cds.
AB591030 - Synthetic construct DNA, clone: pFN21AE1621, Homo sapiens MED10 gene for mediator complex subunit 10, without stop codon, in Flexi system.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9BTT4 (Reactome details) participates in the following event(s):

R-HSA-212352 Formation of ARC coactivator complex
R-HSA-212380 Formation of TRAP coactivator complex
R-HSA-212432 Formation of DRIP coactivator complex
R-HSA-381309 PPARG:RXRA heterodimer binds to fatty acid-like ligands
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-1266738 Developmental Biology
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: C6G491, L6, MED10_HUMAN, NM_032286, NP_115662, Q9BTT4, TRG17, TRG20
UCSC ID: uc003jdo.3
RefSeq Accession: NM_032286
Protein: Q9BTT4 (aka MED10_HUMAN)
CCDS: CCDS34134.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_032286.2
exon count: 4CDS single in 3' UTR: no RNA size: 1059
ORF size: 408CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1016.00frame shift in genome: no % Coverage: 97.07
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.