Human Gene KLHL15 (uc004dba.4)
  Description: Homo sapiens kelch-like family member 15 (KLHL15), mRNA.
RefSeq Summary (NM_030624): This gene encodes a member of the kelch-like family of proteins that share a common domain structure consisting of an N-terminal broad-complex, tramtrack, bric-a-brac/poxvirus and zinc finger domain and C-terminal kelch repeat motifs. The encoded protein may be involved in protein ubiquitination and cytoskeletal organization. [provided by RefSeq, Apr 2009].
Transcript (Including UTRs)
   Position: hg19 chrX:24,001,833-24,045,303 Size: 43,471 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg19 chrX:24,006,038-24,024,810 Size: 18,773 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:24,001,833-24,045,303)mRNA (may differ from genome)Protein (604 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
H-INVHGNCLynxMalacardsMGIneXtProt
OMIMPubMedTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KLH15_HUMAN
DESCRIPTION: RecName: Full=Kelch-like protein 15;
FUNCTION: Probable substrate-specific adapter of an E3 ubiquitin- protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Interacts with CUL3.
SIMILARITY: Contains 1 BACK (BTB/Kelch associated) domain.
SIMILARITY: Contains 1 BTB (POZ) domain.
SIMILARITY: Contains 5 Kelch repeats.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: KLHL15
Diseases sorted by gene-association score: mental retardation, x-linked 103* (919)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 10.06 RPKM in Testis
Total median expression: 114.95 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -103.30256-0.404 Picture PostScript Text
3' UTR -1012.114205-0.241 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011705 - BACK
IPR000210 - BTB/POZ-like
IPR011333 - BTB/POZ_fold
IPR013069 - BTB_POZ
IPR017096 - Kelch-like_gigaxonin
IPR015915 - Kelch-typ_b-propeller
IPR006652 - Kelch_1

Pfam Domains:
PF00651 - BTB/POZ domain
PF01344 - Kelch motif
PF07707 - BTB And C-terminal Kelch
PF13964 - Kelch motif

SCOP Domains:
50965 - Galactose oxidase, central domain
54695 - POZ domain

ModBase Predicted Comparative 3D Structure on Q96M94
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
  Ensembl   
  Protein Sequence   
  Alignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0004842 ubiquitin-protein transferase activity

Biological Process:
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0016567 protein ubiquitination
GO:0071630 nucleus-associated proteasomal ubiquitin-dependent protein catabolic process
GO:2000042 negative regulation of double-strand break repair via homologous recombination

Cellular Component:
GO:0005634 nucleus
GO:0031463 Cul3-RING ubiquitin ligase complex


-  Descriptions from all associated GenBank mRNAs
  LF211573 - JP 2014500723-A/19076: Polycomb-Associated Non-Coding RNAs.
AL834313 - Homo sapiens mRNA; cDNA DKFZp434M0516 (from clone DKFZp434M0516).
AB051464 - Homo sapiens mRNA for KIAA1677 protein, partial cds.
JD327339 - Sequence 308363 from Patent EP1572962.
JD493464 - Sequence 474488 from Patent EP1572962.
JD095699 - Sequence 76723 from Patent EP1572962.
JD311246 - Sequence 292270 from Patent EP1572962.
JD086748 - Sequence 67772 from Patent EP1572962.
JD510655 - Sequence 491679 from Patent EP1572962.
JD110761 - Sequence 91785 from Patent EP1572962.
JD022569 - Sequence 3593 from Patent EP1572962.
JD301921 - Sequence 282945 from Patent EP1572962.
LF381458 - JP 2014500723-A/188961: Polycomb-Associated Non-Coding RNAs.
JD198865 - Sequence 179889 from Patent EP1572962.
JD454361 - Sequence 435385 from Patent EP1572962.
JD245279 - Sequence 226303 from Patent EP1572962.
JD435929 - Sequence 416953 from Patent EP1572962.
JD528277 - Sequence 509301 from Patent EP1572962.
LF381457 - JP 2014500723-A/188960: Polycomb-Associated Non-Coding RNAs.
JD048950 - Sequence 29974 from Patent EP1572962.
JD166568 - Sequence 147592 from Patent EP1572962.
JD488467 - Sequence 469491 from Patent EP1572962.
JD425281 - Sequence 406305 from Patent EP1572962.
JD438911 - Sequence 419935 from Patent EP1572962.
JD503795 - Sequence 484819 from Patent EP1572962.
JD360618 - Sequence 341642 from Patent EP1572962.
JD065410 - Sequence 46434 from Patent EP1572962.
JD197530 - Sequence 178554 from Patent EP1572962.
JD429397 - Sequence 410421 from Patent EP1572962.
JD067983 - Sequence 49007 from Patent EP1572962.
JD156142 - Sequence 137166 from Patent EP1572962.
JD300529 - Sequence 281553 from Patent EP1572962.
JD082499 - Sequence 63523 from Patent EP1572962.
BC015415 - Homo sapiens kelch-like 15 (Drosophila), mRNA (cDNA clone IMAGE:4395166), partial cds.
JD261724 - Sequence 242748 from Patent EP1572962.
JD066872 - Sequence 47896 from Patent EP1572962.
JD080327 - Sequence 61351 from Patent EP1572962.
JD440424 - Sequence 421448 from Patent EP1572962.
JD330590 - Sequence 311614 from Patent EP1572962.
JD082836 - Sequence 63860 from Patent EP1572962.
JD433635 - Sequence 414659 from Patent EP1572962.
AK057298 - Homo sapiens cDNA FLJ32736 fis, clone TESTI2001237, weakly similar to RING CANAL PROTEIN.
BC109058 - Homo sapiens kelch-like 15 (Drosophila), mRNA (cDNA clone MGC:126148 IMAGE:40033682), complete cds.
BC109059 - Homo sapiens kelch-like 15 (Drosophila), mRNA (cDNA clone MGC:126149 IMAGE:40033683), complete cds.
EU794630 - Homo sapiens epididymis secretory protein Li 305 (HEL-S-305) mRNA, complete cds.
GQ891312 - Homo sapiens clone HEL-S-35a epididymis secretory sperm binding protein mRNA, complete cds.
KJ894811 - Synthetic construct Homo sapiens clone ccsbBroadEn_04205 KLHL15 gene, encodes complete protein.
AB384623 - Synthetic construct DNA, clone: pF1KA1677, Homo sapiens KLHL15 gene for kelch-like protein 15, complete cds, without stop codon, in Flexi system.
LF381456 - JP 2014500723-A/188959: Polycomb-Associated Non-Coding RNAs.
LF381445 - JP 2014500723-A/188948: Polycomb-Associated Non-Coding RNAs.
JD235418 - Sequence 216442 from Patent EP1572962.
MA447150 - JP 2018138019-A/19076: Polycomb-Associated Non-Coding RNAs.
MA617035 - JP 2018138019-A/188961: Polycomb-Associated Non-Coding RNAs.
MA617034 - JP 2018138019-A/188960: Polycomb-Associated Non-Coding RNAs.
MA617033 - JP 2018138019-A/188959: Polycomb-Associated Non-Coding RNAs.
MA617022 - JP 2018138019-A/188948: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: KIAA1677, KLH15_HUMAN, NM_030624, NP_085127, Q32MN3, Q8NDA3, Q96BM6, Q96M94, Q9C0I6
UCSC ID: uc004dba.4
RefSeq Accession: NM_030624
Protein: Q96M94 (aka KLH15_HUMAN)
CCDS: CCDS35217.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_030624.2
exon count: 4CDS single in 3' UTR: no RNA size: 6290
ORF size: 1815CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3819.50frame shift in genome: no % Coverage: 99.78
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.