Human Gene DPH7 (uc031tfx.1)
  Description: Homo sapiens WD repeat domain 85 (DPH7), mRNA.
RefSeq Summary (NM_138778): Diphthamide is a post-translationally modified histidine residue present in elongation factor 2, and is the target of diphtheria toxin. This gene encodes a protein that contains a WD-40 domain, and is thought to be involved in diphthamide biosynthesis. A similar protein in yeast functions as a methylesterase, converting methylated diphthine to diphthine, which can then undergo amidation to produce diphthamide. [provided by RefSeq, Oct 2016].
Transcript (Including UTRs)
   Position: hg19 chr9:140,458,939-140,473,387 Size: 14,449 Total Exon Count: 5 Strand: -
Coding Region
   Position: hg19 chr9:140,459,186-140,468,730 Size: 9,545 Coding Exon Count: 2 

Page IndexSequence and LinksPrimersMalaCardsGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureOther SpeciesmRNA DescriptionsOther NamesModel Information
Methods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:140,458,939-140,473,387)mRNA (may differ from genome)Protein (163 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
EnsemblExonPrimerGeneCardsGeneNetworkH-INVLynx
MalacardsMGIPubMed

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: DPH7
Diseases sorted by gene-association score: diphtheria (5)

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 15.47 RPKM in Prostate
Total median expression: 426.44 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -285.90593-0.482 Picture PostScript Text
3' UTR -95.20247-0.385 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Descriptions from all associated GenBank mRNAs
  BC040173 - Homo sapiens WD repeat domain 85, mRNA (cDNA clone MGC:41963 IMAGE:4791980), complete cds.
BC003123 - Homo sapiens WD repeat domain 85, mRNA (cDNA clone MGC:3209 IMAGE:3502107), complete cds.
BC017335 - Homo sapiens WD repeat domain 85, mRNA (cDNA clone MGC:29782 IMAGE:4642600), complete cds.
BC016349 - Homo sapiens WD repeat domain 85, mRNA (cDNA clone IMAGE:4071233), with apparent retained intron.
AK075115 - Homo sapiens cDNA FLJ90634 fis, clone PLACE1003598, weakly similar to TRP-ASP REPEATS CONTAINING PROTEIN RBA-1.
HQ448236 - Synthetic construct Homo sapiens clone IMAGE:100071637; CCSB007843_03 WD repeat domain 85 (WDR85) gene, encodes complete protein.
KJ895195 - Synthetic construct Homo sapiens clone ccsbBroadEn_04589 WDR85 gene, encodes complete protein.
KJ904948 - Synthetic construct Homo sapiens clone ccsbBroadEn_14342 WDR85 gene, encodes complete protein.
AK310226 - Homo sapiens cDNA, FLJ17268.
CU675946 - Synthetic construct Homo sapiens gateway clone IMAGE:100017300 5' read WDR85 mRNA.
AK308998 - Homo sapiens cDNA, FLJ99039.
AK307180 - Homo sapiens cDNA, FLJ97128.
AK310987 - Homo sapiens cDNA, FLJ18029.
JD055041 - Sequence 36065 from Patent EP1572962.
JD336050 - Sequence 317074 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: AK308998, uc004cnm.1, WDR85
UCSC ID: uc031tfx.1
RefSeq Accession: NM_138778

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: AK308998.1
exon count: 5CDS single in 3' UTR: no RNA size: 1321
ORF size: 492CDS single in intron: no Alignment % ID: 99.92
txCdsPredict score: 724.50frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: yes # AT/AC introns 0
selenocysteine: no end bleed into intron: 406# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.