Human Gene KLHL28 (uc001wvr.3)
  Description: Homo sapiens kelch-like family member 28 (KLHL28), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr14:45,393,527-45,431,179 Size: 37,653 Total Exon Count: 5 Strand: -
Coding Region
   Position: hg19 chr14:45,398,231-45,415,131 Size: 16,901 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:45,393,527-45,431,179)mRNA (may differ from genome)Protein (571 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkHGNCHPRDLynxMGIneXtProt
PubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KLH28_HUMAN
DESCRIPTION: RecName: Full=Kelch-like protein 28; AltName: Full=BTB/POZ domain-containing protein 5;
SIMILARITY: Contains 1 BTB (POZ) domain.
SIMILARITY: Contains 6 Kelch repeats.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 3.12 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 96.24 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -46.80131-0.357 Picture PostScript Text
3' UTR -1153.534704-0.245 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011705 - BACK
IPR000210 - BTB/POZ-like
IPR011333 - BTB/POZ_fold
IPR013069 - BTB_POZ
IPR017096 - Kelch-like_gigaxonin
IPR015915 - Kelch-typ_b-propeller
IPR006652 - Kelch_1

Pfam Domains:
PF00651 - BTB/POZ domain
PF01344 - Kelch motif
PF07646 - Kelch motif
PF07707 - BTB And C-terminal Kelch
PF13964 - Kelch motif

SCOP Domains:
101898 - NHL repeat
50965 - Galactose oxidase, central domain
54695 - POZ domain

ModBase Predicted Comparative 3D Structure on Q9NXS3
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
  Ensembl   
  Protein Sequence   
  Alignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004842 ubiquitin-protein transferase activity

Biological Process:
GO:0016567 protein ubiquitination

Cellular Component:
GO:0031463 Cul3-RING ubiquitin ligase complex


-  Descriptions from all associated GenBank mRNAs
  AK090972 - Homo sapiens cDNA FLJ33653 fis, clone BRAMY2024715.
JD144574 - Sequence 125598 from Patent EP1572962.
JD275238 - Sequence 256262 from Patent EP1572962.
JD497081 - Sequence 478105 from Patent EP1572962.
AK289855 - Homo sapiens cDNA FLJ76338 complete cds, highly similar to Homo sapiens BTB (POZ) domain containing 5 (BTBD5), mRNA.
BC057794 - Homo sapiens cDNA clone IMAGE:5295395, containing frame-shift errors.
BC121009 - Homo sapiens kelch-like 28 (Drosophila), mRNA (cDNA clone MGC:149636 IMAGE:40116967), complete cds.
BC121010 - Homo sapiens kelch-like 28 (Drosophila), mRNA (cDNA clone MGC:149637 IMAGE:40116970), complete cds.
KJ894068 - Synthetic construct Homo sapiens clone ccsbBroadEn_03462 KLHL28 gene, encodes complete protein.
KJ906201 - Synthetic construct Homo sapiens clone ccsbBroadEn_15871 KLHL28 gene, encodes complete protein.
BC012473 - Homo sapiens cDNA clone IMAGE:4453077, containing frame-shift errors.
AK000088 - Homo sapiens cDNA FLJ20081 fis, clone COL03242.
AK225183 - Homo sapiens mRNA for BTB (POZ) domain containing 5 variant, clone: COL03244.
AK225211 - Homo sapiens mRNA for BTB (POZ) domain containing 5 variant, clone: COL03242.
JD296441 - Sequence 277465 from Patent EP1572962.
JD327479 - Sequence 308503 from Patent EP1572962.
JD564165 - Sequence 545189 from Patent EP1572962.
JD185826 - Sequence 166850 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: BTBD5, KLH28_HUMAN, NM_017658, NP_060128, Q0VAL5, Q9NXS3
UCSC ID: uc001wvr.3
RefSeq Accession: NM_017658
Protein: Q9NXS3 (aka KLH28_HUMAN)
CCDS: CCDS9680.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_017658.3
exon count: 5CDS single in 3' UTR: no RNA size: 6568
ORF size: 1716CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3632.00frame shift in genome: no % Coverage: 99.74
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.