Description: Homo sapiens polymerase (DNA directed), epsilon 3, accessory subunit (POLE3), transcript variant 1, mRNA. RefSeq Summary (NM_001278255): POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]. Transcript (Including UTRs) Position: hg19 chr9:116,169,518-116,173,029 Size: 3,512 Total Exon Count: 5 Strand: - Coding Region Position: hg19 chr9:116,171,091-116,172,573 Size: 1,483 Coding Exon Count: 4
ID:DPOE3_HUMAN DESCRIPTION: RecName: Full=DNA polymerase epsilon subunit 3; EC=2.7.7.7; AltName: Full=Arsenic-transactivated protein; Short=AsTP; AltName: Full=Chromatin accessibility complex 17 kDa protein; Short=CHRAC-17; Short=HuCHRAC17; AltName: Full=DNA polymerase II subunit 3; AltName: Full=DNA polymerase epsilon subunit p17; FUNCTION: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). SUBUNIT: Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4. Interaction with POLE4 is a prerequisite for further binding with POLE and POLE2. Interacts with CHRAC1. Together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRAC. INTERACTION: Q9Q2G4:ORF (xeno); NbExp=5; IntAct=EBI-744901, EBI-6248094; Q9NR33:POLE4; NbExp=3; IntAct=EBI-744901, EBI-867034; SUBCELLULAR LOCATION: Nucleus (Potential). TISSUE SPECIFICITY: Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/pole3/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9NRF9
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000082 G1/S transition of mitotic cell cycle GO:0006260 DNA replication GO:0043966 histone H3 acetylation GO:0071897 DNA biosynthetic process
AK074629 - Homo sapiens cDNA FLJ90148 fis, clone HEMBB1001959, weakly similar to CCAAT-BINDING TRANSCRIPTION FACTOR SUBUNIT A. AK096050 - Homo sapiens cDNA FLJ38731 fis, clone KIDNE2010739, moderately similar to Homo sapiens CHRAC17 (CHRAC17) mRNA. AF070640 - Homo sapiens clone 24781 mRNA sequence. BC003166 - Homo sapiens polymerase (DNA directed), epsilon 3 (p17 subunit), mRNA (cDNA clone MGC:4348 IMAGE:2822216), complete cds. BC004170 - Homo sapiens polymerase (DNA directed), epsilon 3 (p17 subunit), mRNA (cDNA clone MGC:2725 IMAGE:2822216), complete cds. AK092840 - Homo sapiens cDNA FLJ35521 fis, clone SPLEN2001278, highly similar to Homo sapiens CHRAC17 (CHRAC17) mRNA. AK074782 - Homo sapiens cDNA FLJ90301 fis, clone NT2RP2000610, weakly similar to CCAAT-BINDING TRANSCRIPTION FACTOR SUBUNIT A. AK074762 - Homo sapiens cDNA FLJ90281 fis, clone NT2RP1000468, weakly similar to CCAAT-BINDING TRANSCRIPTION FACTOR SUBUNIT A. JD296304 - Sequence 277328 from Patent EP1572962. JD550529 - Sequence 531553 from Patent EP1572962. JD353672 - Sequence 334696 from Patent EP1572962. JD070593 - Sequence 51617 from Patent EP1572962. JD406385 - Sequence 387409 from Patent EP1572962. JD425945 - Sequence 406969 from Patent EP1572962. JD255863 - Sequence 236887 from Patent EP1572962. JD454712 - Sequence 435736 from Patent EP1572962. JD292847 - Sequence 273871 from Patent EP1572962. JD562851 - Sequence 543875 from Patent EP1572962. JD551364 - Sequence 532388 from Patent EP1572962. JD148686 - Sequence 129710 from Patent EP1572962. JD202203 - Sequence 183227 from Patent EP1572962. JD160224 - Sequence 141248 from Patent EP1572962. JD247760 - Sequence 228784 from Patent EP1572962. JD404172 - Sequence 385196 from Patent EP1572962. JD525868 - Sequence 506892 from Patent EP1572962. JD399753 - Sequence 380777 from Patent EP1572962. JD424439 - Sequence 405463 from Patent EP1572962. JD536793 - Sequence 517817 from Patent EP1572962. JD255371 - Sequence 236395 from Patent EP1572962. JD392307 - Sequence 373331 from Patent EP1572962. JD214118 - Sequence 195142 from Patent EP1572962. JD254475 - Sequence 235499 from Patent EP1572962. JD123885 - Sequence 104909 from Patent EP1572962. JD021812 - Sequence 2836 from Patent EP1572962. JD027916 - Sequence 8940 from Patent EP1572962. JD469593 - Sequence 450617 from Patent EP1572962. JD073193 - Sequence 54217 from Patent EP1572962. JD381058 - Sequence 362082 from Patent EP1572962. JD316987 - Sequence 298011 from Patent EP1572962. JD313207 - Sequence 294231 from Patent EP1572962. JD453930 - Sequence 434954 from Patent EP1572962. JD083761 - Sequence 64785 from Patent EP1572962. AF226077 - Homo sapiens CHRAC17 (CHRAC17) mRNA, complete cds. AY720898 - Homo sapiens arsenic transactivated protein mRNA, complete cds. JF432738 - Synthetic construct Homo sapiens clone IMAGE:100073982 polymerase (DNA directed), epsilon 3 (p17 subunit) (POLE3) gene, encodes complete protein. KJ894010 - Synthetic construct Homo sapiens clone ccsbBroadEn_03404 POLE3 gene, encodes complete protein. AB527738 - Synthetic construct DNA, clone: pF1KB7911, Homo sapiens POLE3 gene for polymerase (DNA directed), epsilon 3, without stop codon, in Flexi system.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa00230 - Purine metabolism hsa00240 - Pyrimidine metabolism hsa01100 - Metabolic pathways hsa03030 - DNA replication hsa03410 - Base excision repair hsa03420 - Nucleotide excision repair
Reactome (by CSHL, EBI, and GO)
Protein Q9NRF9 (Reactome details) participates in the following event(s):
R-HSA-68960 DNA polymerase epsilon binds at the origin R-HSA-68913 The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer at the origin R-HSA-174425 The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer on the G strand of the telomere R-HSA-68914 DNA polymerase alpha:primase binds at the origin R-HSA-5653838 POLD,POLE binds deISGylated PCNA after TLS R-HSA-5652009 RAD18:UBE2B or RBX1:CUL4:DDB1:DTL monoubiquitinates PCNA R-HSA-110364 PCNA:POLD,POLE:RPA:RFC and FEN1 bind APEX1 R-HSA-5651992 PCNA-containing replication complex binds damaged dsDNA R-HSA-5651809 LIG1, APEX1 and PCNA:POLD,POLE:RPA:RFC dissociate from repaired DNA R-HSA-5653840 POLD,POLE complete replication of damaged DNA after TLS R-HSA-5690213 DNA polymerases delta, epsilon or kappa bind the GG-NER site R-HSA-6782211 DNA polymerases delta, epsilon or kappa bind the TC-NER site R-HSA-110368 POLD,POLE-mediated DNA strand displacement synthesis R-HSA-110371 LIG1 binds APEX1 and PCNA at SSB R-HSA-110363 FEN1 bound to PCNA and APEX1 cleaves flap ssDNA R-HSA-5651805 LIG1 bound to APEX1 and PCNA ligates SSB R-HSA-5652005 RAD18:UBE2B or RBX1:CUL4:DDB1:DTL ubiquitin ligase complex binds PCNA:POLD,POLE:RPA:RFC associated with damaged dsDNA R-HSA-5693593 D-loop extension by DNA polymerases R-HSA-5690997 Ligation of newly synthesized repair patch to incised DNA in GG-NER R-HSA-6782227 Ligation of newly synthesized repair patch to incised DNA in TC-NER R-HSA-5690988 3'-incision of DNA by ERCC5 (XPG) in GG-NER R-HSA-5691001 Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in GG-NER R-HSA-6782224 3' incision by ERCC5 (XPG) in TC-NER R-HSA-6782208 Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER R-HSA-68962 Activation of the pre-replicative complex R-HSA-68952 DNA replication initiation R-HSA-174430 Telomere C-strand synthesis initiation R-HSA-5656169 Termination of translesion DNA synthesis R-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex R-HSA-69002 DNA Replication Pre-Initiation R-HSA-69206 G1/S Transition R-HSA-69239 Synthesis of DNA R-HSA-174417 Telomere C-strand (Lagging Strand) Synthesis R-HSA-5651801 PCNA-Dependent Long Patch Base Excision Repair R-HSA-5696400 Dual Incision in GG-NER R-HSA-6782135 Dual incision in TC-NER R-HSA-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template R-HSA-73893 DNA Damage Bypass R-HSA-68874 M/G1 Transition R-HSA-453279 Mitotic G1-G1/S phases R-HSA-69242 S Phase R-HSA-69306 DNA Replication R-HSA-180786 Extension of Telomeres R-HSA-110373 Resolution of AP sites via the multiple-nucleotide patch replacement pathway R-HSA-5696399 Global Genome Nucleotide Excision Repair (GG-NER) R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER) R-HSA-73894 DNA Repair R-HSA-69278 Cell Cycle (Mitotic) R-HSA-157579 Telomere Maintenance R-HSA-5685942 HDR through Homologous Recombination (HRR) R-HSA-73933 Resolution of Abasic Sites (AP sites) R-HSA-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER R-HSA-5696398 Nucleotide Excision Repair R-HSA-1640170 Cell Cycle R-HSA-73886 Chromosome Maintenance R-HSA-5693567 HDR through Homologous Recombination (HR) or Single Strand Annealing (SSA) R-HSA-73884 Base Excision Repair R-HSA-5693538 Homology Directed Repair R-HSA-5693532 DNA Double-Strand Break Repair
US Server
