Human Gene TRABD2B (uc021ong.1)
  Description: Homo sapiens TraB domain containing 2B (TRABD2B), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr1:48,226,200-48,462,562 Size: 236,363 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr1:48,231,574-48,462,461 Size: 230,888 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:48,226,200-48,462,562)mRNA (may differ from genome)Protein (517 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
LynxMGIneXtProtOMIMPubMedTreefam
UniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TIKI2_HUMAN
DESCRIPTION: RecName: Full=Metalloprotease TIKI2; EC=3.4.-.-; AltName: Full=TRAB domain-containing protein 2B; Flags: Precursor;
FUNCTION: Metalloprotease that acts as a negative regulator of the Wnt signaling pathway by mediating the cleavage of the 8 N- terminal residues of a subset of Wnt proteins. Following cleavage, Wnt proteins become oxidized and form large disulfide-bond oligomers, leading to their inactivation. Able to cleave WNT3A, WNT5, but not WNT11. Required for head formation.
COFACTOR: Divalent metal cations. Manganese or cobalt.
ENZYME REGULATION: Inhibited by 1,10-phenanthroline, a metalloprotease inhibitor which is a divalent metal chelator. Also inhibited by EDTA. Not inhibited by Bestatin, an aminopeptidase inhibitor, nor to a mixture of inhibitors for serine, cysteine, and aspartic proteases and aminopeptidases.
INTERACTION: P27467:Wnt3a (xeno); NbExp=2; IntAct=EBI-6257471, EBI-2899665;
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.
MISCELLANEOUS: Was named TIKI in reference to large-headed humanoid in Polynesian mythology (PubMed:22726442).
SIMILARITY: Belongs to the TIKI family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 29.56 RPKM in Artery - Tibial
Total median expression: 154.73 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -51.50101-0.510 Picture PostScript Text
3' UTR -2050.805374-0.382 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002816 - Pheromone_shutdown_TraB

Pfam Domains:
PF01963 - TraB family

ModBase Predicted Comparative 3D Structure on A6NFA1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserNo orthologGenome BrowserNo ortholog
Gene Details   Gene Details 
Gene Sorter   Gene Sorter 
  Ensembl WormBase 
  Protein Sequence Protein Sequence 
  Alignment Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004222 metalloendopeptidase activity
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0016787 hydrolase activity
GO:0017147 Wnt-protein binding
GO:0046872 metal ion binding

Biological Process:
GO:0006508 proteolysis
GO:0016055 Wnt signaling pathway
GO:0030178 negative regulation of Wnt signaling pathway
GO:0032461 positive regulation of protein oligomerization
GO:1904808 positive regulation of protein oxidation

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0031301 integral component of organelle membrane


-  Descriptions from all associated GenBank mRNAs
  JQ653416 - Homo sapiens TIKI2 (TIKI2) mRNA, complete cds.
AK090467 - Homo sapiens mRNA for FLJ00388 protein.
JD268784 - Sequence 249808 from Patent EP1572962.
JD368230 - Sequence 349254 from Patent EP1572962.
JD340259 - Sequence 321283 from Patent EP1572962.
JD444470 - Sequence 425494 from Patent EP1572962.
JD283057 - Sequence 264081 from Patent EP1572962.
JD112844 - Sequence 93868 from Patent EP1572962.
JD202161 - Sequence 183185 from Patent EP1572962.
JD332733 - Sequence 313757 from Patent EP1572962.
JD495354 - Sequence 476378 from Patent EP1572962.
JD500655 - Sequence 481679 from Patent EP1572962.
JD454265 - Sequence 435289 from Patent EP1572962.
JD194424 - Sequence 175448 from Patent EP1572962.
JD422908 - Sequence 403932 from Patent EP1572962.
JD173228 - Sequence 154252 from Patent EP1572962.
JD530129 - Sequence 511153 from Patent EP1572962.
JD195281 - Sequence 176305 from Patent EP1572962.
JD555936 - Sequence 536960 from Patent EP1572962.
JD323611 - Sequence 304635 from Patent EP1572962.
JD264361 - Sequence 245385 from Patent EP1572962.
JD225518 - Sequence 206542 from Patent EP1572962.
JD347261 - Sequence 328285 from Patent EP1572962.
JD484273 - Sequence 465297 from Patent EP1572962.
JD044712 - Sequence 25736 from Patent EP1572962.
JD045604 - Sequence 26628 from Patent EP1572962.
JD507340 - Sequence 488364 from Patent EP1572962.
JD352285 - Sequence 333309 from Patent EP1572962.
JD178619 - Sequence 159643 from Patent EP1572962.
JD037347 - Sequence 18371 from Patent EP1572962.
JD281853 - Sequence 262877 from Patent EP1572962.
JD429201 - Sequence 410225 from Patent EP1572962.
JD061773 - Sequence 42797 from Patent EP1572962.
JD275399 - Sequence 256423 from Patent EP1572962.
JD312673 - Sequence 293697 from Patent EP1572962.
JD437329 - Sequence 418353 from Patent EP1572962.
JD101589 - Sequence 82613 from Patent EP1572962.
JD394324 - Sequence 375348 from Patent EP1572962.
JD200161 - Sequence 181185 from Patent EP1572962.
JD346897 - Sequence 327921 from Patent EP1572962.
JD443335 - Sequence 424359 from Patent EP1572962.
JD087650 - Sequence 68674 from Patent EP1572962.
JD411649 - Sequence 392673 from Patent EP1572962.
JD245018 - Sequence 226042 from Patent EP1572962.
JD318814 - Sequence 299838 from Patent EP1572962.
JD425641 - Sequence 406665 from Patent EP1572962.
JD474207 - Sequence 455231 from Patent EP1572962.
JD259472 - Sequence 240496 from Patent EP1572962.
JD411260 - Sequence 392284 from Patent EP1572962.
JD080618 - Sequence 61642 from Patent EP1572962.
JD507464 - Sequence 488488 from Patent EP1572962.
JD133883 - Sequence 114907 from Patent EP1572962.
JD234504 - Sequence 215528 from Patent EP1572962.
JD363126 - Sequence 344150 from Patent EP1572962.
JD131364 - Sequence 112388 from Patent EP1572962.
JD264537 - Sequence 245561 from Patent EP1572962.
JD119521 - Sequence 100545 from Patent EP1572962.
JD383643 - Sequence 364667 from Patent EP1572962.
JD505023 - Sequence 486047 from Patent EP1572962.
JD483265 - Sequence 464289 from Patent EP1572962.
JD210224 - Sequence 191248 from Patent EP1572962.
JD092690 - Sequence 73714 from Patent EP1572962.
JD474852 - Sequence 455876 from Patent EP1572962.
JD164439 - Sequence 145463 from Patent EP1572962.
JD324916 - Sequence 305940 from Patent EP1572962.
JD400353 - Sequence 381377 from Patent EP1572962.
JD512012 - Sequence 493036 from Patent EP1572962.
JD407748 - Sequence 388772 from Patent EP1572962.
JD256876 - Sequence 237900 from Patent EP1572962.
JD362760 - Sequence 343784 from Patent EP1572962.
JD471638 - Sequence 452662 from Patent EP1572962.
JD323136 - Sequence 304160 from Patent EP1572962.
JD039054 - Sequence 20078 from Patent EP1572962.
JD440549 - Sequence 421573 from Patent EP1572962.
JD039493 - Sequence 20517 from Patent EP1572962.
JD267865 - Sequence 248889 from Patent EP1572962.
JD530197 - Sequence 511221 from Patent EP1572962.
JD154938 - Sequence 135962 from Patent EP1572962.
JD450162 - Sequence 431186 from Patent EP1572962.
JD360934 - Sequence 341958 from Patent EP1572962.
JD236083 - Sequence 217107 from Patent EP1572962.
JD121526 - Sequence 102550 from Patent EP1572962.
JD544933 - Sequence 525957 from Patent EP1572962.
JD396683 - Sequence 377707 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A6NFA1, I6U4Y0, NM_001194986, NP_001181915, TIKI2, TIKI2_HUMAN
UCSC ID: uc021ong.1
RefSeq Accession: NM_001194986
Protein: A6NFA1 (aka TIKI2_HUMAN)
CCDS: CCDS58000.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001194986.1
exon count: 7CDS single in 3' UTR: no RNA size: 7029
ORF size: 1554CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3308.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.