Human Gene PRKAR1B (uc003siw.2)
  Description: Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 3, mRNA.
RefSeq Summary (NM_001164758): The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2015].
Transcript (Including UTRs)
   Position: hg19 chr7:588,834-767,313 Size: 178,480 Total Exon Count: 11 Strand: -
Coding Region
   Position: hg19 chr7:590,067-751,142 Size: 161,076 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsMalaCards
CTDGene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein Structure
Other SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther NamesModel Information
Methods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:588,834-767,313)mRNA (may differ from genome)Protein (381 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
H-INVHGNCHPRDLynxMalacardsMGI
neXtProtOMIMPubMedReactomeUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KAP1_HUMAN
DESCRIPTION: RecName: Full=cAMP-dependent protein kinase type I-beta regulatory subunit;
FUNCTION: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
SUBUNIT: The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase.
TISSUE SPECIFICITY: Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
PTM: The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
SIMILARITY: Belongs to the cAMP-dependent kinase regulatory chain family.
SIMILARITY: Contains 2 cyclic nucleotide-binding domains.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): PRKAR1B
CDC HuGE Published Literature: PRKAR1B

-  MalaCards Disease Associations
  MalaCards Gene Search: PRKAR1B
Diseases sorted by gene-association score: prkar1b-related neurodegenerative dementia with intermediate filaments* (350), carney complex variant (5), familial atrial fibrillation (1)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 153.30 RPKM in Brain - Cortex
Total median expression: 1086.57 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -32.00109-0.294 Picture PostScript Text
3' UTR -545.891233-0.443 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002373 - cAMP/cGMP_kin
IPR012198 - cAMP_dep_PK_reg_su
IPR003117 - cAMP_dep_PK_reg_su_I/II_a/b
IPR018490 - cNMP-bd-like
IPR018488 - cNMP-bd_CS
IPR000595 - cNMP-bd_dom
IPR014710 - RmlC-like_jellyroll

Pfam Domains:
PF00027 - Cyclic nucleotide-binding domain
PF02197 - Regulatory subunit of type II PKA R-subunit

SCOP Domains:
51206 - cAMP-binding domain-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
4DIN - X-ray MuPIT 4F9K - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P31321
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserGenome BrowserGenome BrowserNo ortholog
Gene DetailsGene Details Gene DetailsGene Details 
Gene SorterGene Sorter Gene SorterGene Sorter 
 RGDEnsemblFlyBaseWormBase 
 Protein SequenceProtein SequenceProtein SequenceProtein Sequence 
 AlignmentAlignmentAlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004862 cAMP-dependent protein kinase inhibitor activity
GO:0005515 protein binding
GO:0008603 cAMP-dependent protein kinase regulator activity
GO:0030552 cAMP binding
GO:0034236 protein kinase A catalytic subunit binding

Biological Process:
GO:0001932 regulation of protein phosphorylation
GO:0003091 renal water homeostasis
GO:0006468 protein phosphorylation
GO:0007596 blood coagulation
GO:0007611 learning or memory
GO:0034199 activation of protein kinase A activity
GO:0071377 cellular response to glucagon stimulus
GO:2000480 negative regulation of cAMP-dependent protein kinase activity

Cellular Component:
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0005952 cAMP-dependent protein kinase complex
GO:0016020 membrane
GO:0097546 ciliary base


-  Descriptions from all associated GenBank mRNAs
  BC013368 - Homo sapiens cDNA clone IMAGE:3677877, containing frame-shift errors.
AL833563 - Homo sapiens mRNA; cDNA DKFZp686J1344 (from clone DKFZp686J1344).
M65066 - Human cAMP-dependent protein kinase regulatory subunit RI-beta mRNA, 3' end.
BC026734 - Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, beta, mRNA (cDNA clone MGC:34656 IMAGE:5141110), complete cds.
BC036828 - Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, beta, mRNA (cDNA clone MGC:46241 IMAGE:5247772), complete cds.
AK289464 - Homo sapiens cDNA FLJ77959 complete cds, highly similar to Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), mRNA.
AK297337 - Homo sapiens cDNA FLJ52674 complete cds, highly similar to cAMP-dependent protein kinase type I-beta regulatory subunit.
AK098822 - Homo sapiens cDNA FLJ25956 fis, clone TST03939, highly similar to CAMP-DEPENDENT PROTEIN KINASE TYPE I-BETA REGULATORY CHAIN.
AK315990 - Homo sapiens cDNA, FLJ78889 complete cds, highly similar to cAMP-dependent protein kinase type I-beta regulatory subunit.
AK315951 - Homo sapiens cDNA, FLJ78850 complete cds, highly similar to cAMP-dependent protein kinase type I-beta regulatory subunit.
EU832098 - Synthetic construct Homo sapiens clone HAIB:100067127; DKFZo008A1125 protein kinase, cAMP-dependent, regulatory, type I, beta protein (PRKAR1B) gene, encodes complete protein.
EU832191 - Synthetic construct Homo sapiens clone HAIB:100067220; DKFZo004A1126 protein kinase, cAMP-dependent, regulatory, type I, beta protein (PRKAR1B) gene, encodes complete protein.
AB385544 - Synthetic construct DNA, clone: pF1KB5260, Homo sapiens PRKAR1B gene for cAMP-dependent protein kinase type I-beta regulatory subunit, complete cds, without stop codon, in Flexi system.
BC002470 - Homo sapiens mRNA similar to protein kinase, cAMP dependent regulatory, type I beta (cDNA clone IMAGE:3349336).
JD030269 - Sequence 11293 from Patent EP1572962.
JD021365 - Sequence 2389 from Patent EP1572962.
BC070478 - Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, beta, mRNA (cDNA clone IMAGE:6095844), complete cds.
JD408790 - Sequence 389814 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04210 - Apoptosis
hsa04910 - Insulin signaling pathway

BioCarta from NCI Cancer Genome Anatomy Project
h_dreampathway - Repression of Pain Sensation by the Transcriptional Regulator DREAM
h_chrebpPathway - ChREBP regulation by carbohydrates and cAMP
h_GATA3pathway - GATA3 participate in activating the Th2 cytokine genes expression
h_crebPathway - Transcription factor CREB and its extracellular signals
h_gpcrPathway - Signaling Pathway from G-Protein Families
h_igf1rPathway - Multiple antiapoptotic pathways from IGF-1R signaling lead to BAD phosphorylation
h_mPRPathway - How Progesterone Initiates the Oocyte Maturation
h_agpcrPathway - Attenuation of GPCR Signaling
h_badPathway - Regulation of BAD phosphorylation
h_carm1Pathway - Transcription Regulation by Methyltransferase of CARM1
h_no1Pathway - Actions of Nitric Oxide in the Heart
h_stathminPathway - Stathmin and breast cancer resistance to antimicrotubule agents
h_CSKPathway - Activation of Csk by cAMP-dependent Protein Kinase Inhibits Signaling through the T Cell Receptor
h_ck1Pathway - Regulation of ck1/cdk5 by type 1 glutamate receptors
h_pparaPathway - Mechanism of Gene Regulation by Peroxisome Proliferators via PPARa(alpha)
h_shhPathway - Sonic Hedgehog (Shh) Pathway
h_nos1Pathway - Nitric Oxide Signaling Pathway
h_cftrPathway - Cystic fibrosis transmembrane conductance regulator (CFTR) and beta 2 adrenergic receptor (b2AR) pathway
h_nfatPathway - NFAT and Hypertrophy of the heart (Transcription in the broken heart)
h_plcePathway - Phospholipase C-epsilon pathway
h_vipPathway - Neuropeptides VIP and PACAP inhibit the apoptosis of activated T cells
h_mCalpainPathway - mCalpain and friends in Cell motility

Reactome (by CSHL, EBI, and GO)

Protein P31321 (Reactome details) participates in the following event(s):

R-HSA-992708 Dual-specific AKAPs bind type I and II PKA regulatory subunits
R-HSA-8951727 cAMP binds PKA tetramer
R-HSA-111925 cAMP induces dissociation of inactive PKA tetramers
R-HSA-5610749 cAMP dissociates PKA, promoting GLI processing
R-HSA-381707 PKA:AKAP79:IQGAP1 complex dissociates to active PKA subunits in response to cAMP
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
R-HSA-163615 PKA activation
R-HSA-180024 DARPP-32 events
R-HSA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-HSA-164378 PKA activation in glucagon signalling
R-HSA-5610787 Hedgehog 'off' state
R-HSA-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-HSA-109582 Hemostasis
R-HSA-111931 PKA-mediated phosphorylation of CREB
R-HSA-111885 Opioid Signalling
R-HSA-445717 Aquaporin-mediated transport
R-HSA-163359 Glucagon signaling in metabolic regulation
R-HSA-5358351 Signaling by Hedgehog
R-HSA-422356 Regulation of insulin secretion
R-HSA-111933 Calmodulin induced events
R-HSA-418594 G alpha (i) signalling events
R-HSA-382551 Transport of small molecules
R-HSA-163685 Energy Metabolism
R-HSA-162582 Signal Transduction
R-HSA-111997 CaM pathway
R-HSA-388396 GPCR downstream signalling
R-HSA-1430728 Metabolism
R-HSA-111996 Ca-dependent events
R-HSA-1489509 DAG and IP3 signaling
R-HSA-372790 Signaling by GPCR
R-HSA-112043 PLC beta mediated events
R-HSA-9006925 Intracellular signaling by second messengers
R-HSA-112040 G-protein mediated events

-  Other Names for This Gene
  Alternate Gene Symbols: KAP1_HUMAN, NM_001164758, NP_002726, P31321, Q8N422
UCSC ID: uc003siw.2
RefSeq Accession: NM_001164758
Protein: P31321 (aka KAP1_HUMAN)
CCDS: CCDS34579.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001164758.1
exon count: 11CDS single in 3' UTR: no RNA size: 2488
ORF size: 1146CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 2041.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.