Human Gene KIF4A (uc004dyg.3) Description and Page Index
  Description: Homo sapiens kinesin family member 4A (KIF4A), mRNA.
RefSeq Summary (NM_012310): This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.[provided by RefSeq, Mar 2010]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF071592.2, AB208797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374403.4/ ENSP00000363524.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##
Transcript (Including UTRs)
   Position: hg19 chrX:69,509,879-69,640,774 Size: 130,896 Total Exon Count: 31 Strand: +
Coding Region
   Position: hg19 chrX:69,510,309-69,640,115 Size: 129,807 Coding Exon Count: 30 

Page IndexSequence and LinksUniProtKB CommentsMalaCardsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
Genomic Sequence (chrX:69,509,879-69,640,774)mRNA (may differ from genome)Protein (1232 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
neXtProtOMIMPubMedReactomeStanford SOURCETreefam

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Chromosome-associated kinesin KIF4A; AltName: Full=Chromokinesin-A;
FUNCTION: Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.
SUBUNIT: Interacts with unphosphorylated PRC1 during late mitosis.
SUBCELLULAR LOCATION: Nucleus matrix. Cytoplasm, cytoskeleton, spindle. Midbody. Chromosome. Note=Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.
TISSUE SPECIFICITY: Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
SIMILARITY: Belongs to the kinesin-like protein family. Chromokinesin subfamily.
SIMILARITY: Contains 1 kinesin-motor domain.
SEQUENCE CAUTION: Sequence=AAH49218.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence;

-  MalaCards Disease Associations
  MalaCards Gene Search: KIF4A
Diseases sorted by gene-association score: mental retardation, x-linked 100* (950)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.48 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 27.36 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -41.30143-0.289 Picture PostScript Text
3' UTR -203.89659-0.309 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR019821 - Kinesin_motor_CS
IPR001752 - Kinesin_motor_dom

Pfam Domains:
PF00225 - Kinesin motor domain
PF16796 - Microtubule binding

SCOP Domains:
52540 - P-loop containing nucleoside triphosphate hydrolases

ModBase Predicted Comparative 3D Structure on O95239
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 Protein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003677 DNA binding
GO:0003777 microtubule motor activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008017 microtubule binding
GO:0008574 ATP-dependent microtubule motor activity, plus-end-directed

Biological Process:
GO:0000281 mitotic cytokinesis
GO:0006890 retrograde vesicle-mediated transport, Golgi to ER
GO:0006996 organelle organization
GO:0007018 microtubule-based movement
GO:0008089 anterograde axonal transport
GO:0019886 antigen processing and presentation of exogenous peptide antigen via MHC class II
GO:0051256 mitotic spindle midzone assembly

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005694 chromosome
GO:0005737 cytoplasm
GO:0005819 spindle
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0005871 kinesin complex
GO:0005874 microtubule
GO:0005876 spindle microtubule
GO:0016020 membrane
GO:0016363 nuclear matrix
GO:0030496 midbody
GO:0045171 intercellular bridge
GO:1904115 axon cytoplasm

-  Descriptions from all associated GenBank mRNAs
  AK313133 - Homo sapiens cDNA, FLJ93621, highly similar to Homo sapiens kinesin family member 4A (KIF4A), mRNA.
BC003664 - Homo sapiens kinesin family member 4A, mRNA (cDNA clone IMAGE:3010167), partial cds.
BC038459 - Homo sapiens, Similar to kinesin family member 4A, clone IMAGE:5534401, mRNA.
AF071592 - Homo sapiens kinesin superfamily motor KIF4 mRNA, complete cds.
AB208797 - Homo sapiens mRNA for Chromosome-associated kinesin KIF4A variant protein.
BC050548 - Homo sapiens kinesin family member 4A, mRNA (cDNA clone MGC:57639 IMAGE:4538604), complete cds.
AB384408 - Synthetic construct DNA, clone: pF1KSDB3041, Homo sapiens KIF4A gene for kinesin family member 4A, complete cds, without stop codon, in Flexi system.
AJ271784 - Homo sapiens mRNA for Chromokinesin (KIF 4 gene).
AF179308 - Homo sapiens KIF4 (KIF4) mRNA, complete cds.
AF277375 - Homo sapiens chromokinesin mRNA, partial cds.
AK022592 - Homo sapiens cDNA FLJ12530 fis, clone NT2RM4000167, highly similar to Homo sapiens mRNA for Chromokinesin (KIF 4 gene).
AK022717 - Homo sapiens cDNA FLJ12655 fis, clone NT2RM4002109, highly similar to Homo sapiens mRNA for Chromokinesin (KIF 4 gene).
AK024266 - Homo sapiens cDNA FLJ14204 fis, clone NT2RP3003138, highly similar to Chromosome-associated kinesin KIF4A.
JD471915 - Sequence 452939 from Patent EP1572962.
BC049218 - Homo sapiens kinesin family member 4A, mRNA (cDNA clone IMAGE:5497667), partial cds.
BC011801 - Homo sapiens kinesin family member 4A, mRNA (cDNA clone IMAGE:4423083), with apparent retained intron.
LF381705 - JP 2014500723-A/189208: Polycomb-Associated Non-Coding RNAs.
MA617282 - JP 2018138019-A/189208: Polycomb-Associated Non-Coding RNAs.
AK000638 - Homo sapiens cDNA FLJ20631 fis, clone KAT03761, highly similar to AF071592 Homo sapiens kinesin superfamily motor KIF4 mRNA.
JD541147 - Sequence 522171 from Patent EP1572962.
JD494071 - Sequence 475095 from Patent EP1572962.
JD215753 - Sequence 196777 from Patent EP1572962.
JD323715 - Sequence 304739 from Patent EP1572962.
JD183124 - Sequence 164148 from Patent EP1572962.
JD324608 - Sequence 305632 from Patent EP1572962.
JD486941 - Sequence 467965 from Patent EP1572962.
JD179877 - Sequence 160901 from Patent EP1572962.
JD507759 - Sequence 488783 from Patent EP1572962.
JD229047 - Sequence 210071 from Patent EP1572962.
JD256140 - Sequence 237164 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O95239 (Reactome details) participates in the following event(s):

R-HSA-445077 Transport of L1 from C-domain to P-domain
R-HSA-445071 Reinsertion of L1 into the plasma membrane
R-HSA-984671 Chromokinesins form dimers
R-HSA-983266 Kinesins bind microtubules
R-HSA-6811426 Retrograde COPI vesicles bind kinesin and microtubules
R-HSA-6811423 Retrograde vesicle is tethered at the ER by the NRZ complex and t-SNAREs
R-HSA-437239 Recycling pathway of L1
R-HSA-983189 Kinesins
R-HSA-373760 L1CAM interactions
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
R-HSA-422475 Axon guidance
R-HSA-109582 Hemostasis
R-HSA-2132295 MHC class II antigen presentation
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-1266738 Developmental Biology
R-HSA-1280218 Adaptive Immune System
R-HSA-8856688 Golgi-to-ER retrograde transport
R-HSA-168256 Immune System
R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic
R-HSA-199991 Membrane Trafficking
R-HSA-5653656 Vesicle-mediated transport

-  Other Names for This Gene
  Alternate Gene Symbols: B2R7V5, D3DVU4, KIF4, KIF4A_HUMAN, NM_012310, NP_036442, O95239, Q86TN3, Q86XX7, Q9NNY6, Q9NY24, Q9UMW3
UCSC ID: uc004dyg.3
RefSeq Accession: NM_012310
Protein: O95239 (aka KIF4A_HUMAN or KF4A_HUMAN)
CCDS: CCDS14401.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: NM_012310.4
exon count: 31CDS single in 3' UTR: no RNA size: 4504
ORF size: 3699CDS single in intron: no Alignment % ID: 99.98
txCdsPredict score: 7482.00frame shift in genome: no % Coverage: 99.93
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.