Human Gene G3BP1 (uc003lum.3) Description and Page Index
Description: Homo sapiens GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 2, mRNA. RefSeq Summary (NM_198395): This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]. Transcript (Including UTRs) Position: hg19 chr5:151,151,476-151,184,915 Size: 33,440 Total Exon Count: 12 Strand: + Coding Region Position: hg19 chr5:151,166,182-151,183,652 Size: 17,471 Coding Exon Count: 11
ID:G3BP1_HUMAN DESCRIPTION: RecName: Full=Ras GTPase-activating protein-binding protein 1; Short=G3BP-1; EC=184.108.40.206; EC=220.127.116.11; AltName: Full=ATP-dependent DNA helicase VIII; Short=hDH VIII; AltName: Full=GAP SH3 domain-binding protein 1; FUNCTION: May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium- dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. COFACTOR: Magnesium. Required for helicase activity. SUBUNIT: Binds to the SH3 domain of Ras GTPase-activating protein (RASA1) in proliferating cells. No interaction in quiescent cells Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP1 (By similarity). Interacts with USP10, and may regulate it. Forms homodimers and oligomers. SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytosol. Cell membrane. Nucleus. Note=Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS. TISSUE SPECIFICITY: Ubiquitous. DOMAIN: The NTF2 domain mediates multimerization. PTM: Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization. PTM: Arg-435 is dimethylated, probably to asymmetric dimethylarginine. SIMILARITY: Contains 1 NTF2 domain. SIMILARITY: Contains 1 RRM (RNA recognition motif) domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q13283
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.