Human Gene G3BP1 (uc003lum.3) Description and Page Index
  Description: Homo sapiens GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 2, mRNA.
RefSeq Summary (NM_198395): This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
   Position: hg19 chr5:151,151,476-151,184,915 Size: 33,440 Total Exon Count: 12 Strand: +
Coding Region
   Position: hg19 chr5:151,166,182-151,183,652 Size: 17,471 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsCTDGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:151,151,476-151,184,915)mRNA (may differ from genome)Protein (466 aa)
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-  Comments and Description Text from UniProtKB
  ID: G3BP1_HUMAN
DESCRIPTION: RecName: Full=Ras GTPase-activating protein-binding protein 1; Short=G3BP-1; EC=3.6.4.12; EC=3.6.4.13; AltName: Full=ATP-dependent DNA helicase VIII; Short=hDH VIII; AltName: Full=GAP SH3 domain-binding protein 1;
FUNCTION: May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium- dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
COFACTOR: Magnesium. Required for helicase activity.
SUBUNIT: Binds to the SH3 domain of Ras GTPase-activating protein (RASA1) in proliferating cells. No interaction in quiescent cells Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP1 (By similarity). Interacts with USP10, and may regulate it. Forms homodimers and oligomers.
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytosol. Cell membrane. Nucleus. Note=Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
TISSUE SPECIFICITY: Ubiquitous.
DOMAIN: The NTF2 domain mediates multimerization.
PTM: Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
PTM: Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
SIMILARITY: Contains 1 NTF2 domain.
SIMILARITY: Contains 1 RRM (RNA recognition motif) domain.

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 25.83 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 519.26 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -54.50140-0.389 Picture PostScript Text
3' UTR -286.301263-0.227 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002075 - NTF2
IPR018222 - Nuclear_transport_factor_2_euk
IPR012677 - Nucleotide-bd_a/b_plait
IPR000504 - RRM_dom

Pfam Domains:
PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
PF02136 - Nuclear transport factor 2 (NTF2) domain

SCOP Domains:
54427 - NTF2-like
54928 - RNA-binding domain, RBD

Protein Data Bank (PDB) 3-D Structure
MuPIT help

3Q90
- X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q13283
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
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  Protein Sequence   
  Alignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0004003 ATP-dependent DNA helicase activity
GO:0004004 ATP-dependent RNA helicase activity
GO:0004386 helicase activity
GO:0004518 nuclease activity
GO:0004519 endonuclease activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016787 hydrolase activity

Biological Process:
GO:0007265 Ras protein signal transduction
GO:0032508 DNA duplex unwinding
GO:0034063 stress granule assembly
GO:0090090 negative regulation of canonical Wnt signaling pathway
GO:0090305 nucleic acid phosphodiester bond hydrolysis

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0005925 focal adhesion
GO:0010494 cytoplasmic stress granule
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  BX648012 - Homo sapiens mRNA; cDNA DKFZp686L1159 (from clone DKFZp686L1159).
LP895522 - Sequence 386 from Patent EP3253886.
AK303580 - Homo sapiens cDNA FLJ51772 complete cds, highly similar to Ras-GTPase-activating protein-binding protein 1 (EC 3.6.1.-).
AK311635 - Homo sapiens cDNA, FLJ18677.
AK309172 - Homo sapiens cDNA, FLJ99213.
AK312572 - Homo sapiens cDNA, FLJ92947, Homo sapiens Ras-GTPase-activating protein SH3-domain-bindingprotein (G3BP), mRNA.
BC006997 - Homo sapiens GTPase activating protein (SH3 domain) binding protein 1, mRNA (cDNA clone MGC:12272 IMAGE:3950738), complete cds.
AK300098 - Homo sapiens cDNA FLJ50206 complete cds, highly similar to Ras-GTPase-activating protein-binding protein 1 (EC 3.6.1.-).
AK222606 - Homo sapiens mRNA for Ras-GTPase-activating protein SH3-domain-binding protein variant, clone: CAS05140.
BX647869 - Homo sapiens mRNA; cDNA DKFZp313K0921 (from clone DKFZp313K0921).
AK130003 - Homo sapiens cDNA FLJ26493 fis, clone KDN06317, highly similar to Ras-GTPase-activating protein binding protein 1.
U32519 - Human GAP SH3 binding protein mRNA, complete cds.
BC108278 - Homo sapiens GTPase activating protein (SH3 domain) binding protein 1, mRNA (cDNA clone MGC:111040 IMAGE:6051310), complete cds.
JD152555 - Sequence 133579 from Patent EP1572962.
JD427603 - Sequence 408627 from Patent EP1572962.
CU675483 - Synthetic construct Homo sapiens gateway clone IMAGE:100019534 5' read G3BP1 mRNA.
BT019384 - Homo sapiens Ras-GTPase-activating protein SH3-domain-binding protein mRNA, complete cds.
CR536535 - Homo sapiens full open reading frame cDNA clone RZPDo834G0320D for gene G3BP, Ras-GTPase-activating protein SH3-domain-binding protein; complete cds, incl. stopcodon.
DQ893058 - Synthetic construct clone IMAGE:100005688; FLH193150.01X; RZPDo839D0378D Ras-GTPase-activating protein SH3-domain-binding protein (G3BP) gene, encodes complete protein.
DQ896308 - Synthetic construct Homo sapiens clone IMAGE:100010768; FLH193146.01L; RZPDo839D0368D Ras-GTPase-activating protein SH3-domain-binding protein (G3BP) gene, encodes complete protein.
KJ892936 - Synthetic construct Homo sapiens clone ccsbBroadEn_02330 G3BP1 gene, encodes complete protein.
JD025970 - Sequence 6994 from Patent EP1572962.
JD027792 - Sequence 8816 from Patent EP1572962.
DL491584 - Novel nucleic acids.
JD314132 - Sequence 295156 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: G3BP, G3BP1_HUMAN, NM_198395, NP_938405, Q13283
UCSC ID: uc003lum.3
RefSeq Accession: NM_198395
Protein: Q13283 (aka G3BP1_HUMAN)
CCDS: CCDS4319.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_198395.1
exon count: 12CDS single in 3' UTR: no RNA size: 2824
ORF size: 1401CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 2807.00frame shift in genome: no % Coverage: 99.29
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
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-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.