Human Gene NUP133 (uc001htn.3) Description and Page Index
  Description: Homo sapiens nucleoporin 133kDa (NUP133), mRNA.
RefSeq Summary (NM_018230): The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK001676.1, SRR1803613.135039.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261396.6/ ENSP00000261396.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##
Transcript (Including UTRs)
   Position: hg19 chr1:229,577,044-229,644,088 Size: 67,045 Total Exon Count: 26 Strand: -
Coding Region
   Position: hg19 chr1:229,577,651-229,643,996 Size: 66,346 Coding Exon Count: 26 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr1:229,577,044-229,644,088)mRNA (may differ from genome)Protein (1156 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
neXtProtOMIMPubMedReactomeStanford SOURCETreefam

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Nuclear pore complex protein Nup133; AltName: Full=133 kDa nucleoporin; AltName: Full=Nucleoporin Nup133;
FUNCTION: Involved in poly(A)+ RNA transport.
SUBUNIT: Forms part of the Nup160 subcomplex in the nuclear pore which is composed of Nup160, Nup133, Nup107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and Nup153 to the nucleus.
INTERACTION: P57740:NUP107; NbExp=5; IntAct=EBI-295695, EBI-295687;
SUBCELLULAR LOCATION: Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Note=Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores.
SIMILARITY: Belongs to the nucleoporin Nup133 family.
SEQUENCE CAUTION: Sequence=BAA91885.1; Type=Erroneous initiation; Sequence=BAB14106.1; Type=Erroneous initiation;

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): NUP133
CDC HuGE Published Literature: NUP133

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 13.93 RPKM in Nerve - Tibial
Total median expression: 475.62 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -23.6092-0.257 Picture PostScript Text
3' UTR -153.60607-0.253 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007187 - Nucleoporin_Nup133/Nup155_C
IPR014908 - Nucleoporin_Nup133/Nup155_N
IPR015943 - WD40/YVTN_repeat-like_dom

Pfam Domains:
PF03177 - Non-repetitive/WGA-negative nucleoporin C-terminal

Protein Data Bank (PDB) 3-D Structure
MuPIT help

- X-ray MuPIT

- X-ray MuPIT

- X-ray MuPIT
To conserve bandwidth, only the images from the first 3 structures are shown.
3I4R - X-ray MuPIT

ModBase Predicted Comparative 3D Structure on Q8WUM0
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserGenome BrowserNo ortholog
Gene Details  Gene DetailsGene Details 
Gene Sorter  Gene SorterGene Sorter 
  Protein SequenceProtein SequenceProtein Sequence 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0017056 structural constituent of nuclear pore

Biological Process:
GO:0000972 transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery
GO:0006325 chromatin organization
GO:0006355 regulation of transcription, DNA-templated
GO:0006406 mRNA export from nucleus
GO:0006606 protein import into nucleus
GO:0006999 nuclear pore organization
GO:0015031 protein transport
GO:0016032 viral process
GO:0021915 neural tube development
GO:0022008 neurogenesis
GO:0031081 nuclear pore distribution
GO:0048339 paraxial mesoderm development
GO:0051028 mRNA transport
GO:0061053 somite development

Cellular Component:
GO:0000775 chromosome, centromeric region
GO:0000776 kinetochore
GO:0000777 condensed chromosome kinetochore
GO:0005634 nucleus
GO:0005635 nuclear envelope
GO:0005643 nuclear pore
GO:0005694 chromosome
GO:0005829 cytosol
GO:0016020 membrane
GO:0031080 nuclear pore outer ring
GO:0031965 nuclear membrane
GO:0000940 condensed chromosome outer kinetochore

-  Descriptions from all associated GenBank mRNAs
  LF384496 - JP 2014500723-A/191999: Polycomb-Associated Non-Coding RNAs.
AK001676 - Homo sapiens cDNA FLJ10814 fis, clone NT2RP4000984.
AK092339 - Homo sapiens cDNA FLJ35020 fis.
AX747487 - Sequence 1012 from Patent EP1308459.
BC020107 - Homo sapiens nucleoporin 133kDa, mRNA (cDNA clone MGC:21133 IMAGE:3919973), complete cds.
AK304861 - Homo sapiens cDNA FLJ55448 complete cds, highly similar to Nuclear pore complex protein Nup133.
AK304061 - Homo sapiens cDNA FLJ55441 complete cds, highly similar to Nuclear pore complex protein Nup133.
AK314431 - Homo sapiens cDNA, FLJ95222, Homo sapiens nucleoporin 133kDa (NUP133), mRNA.
EU176236 - Synthetic construct Homo sapiens clone IMAGE:100006365; FLH169221.01X; RZPDo839H08250D nucleoporin 133kDa (NUP133) gene, encodes complete protein.
DQ894268 - Synthetic construct Homo sapiens clone IMAGE:100008728; FLH169214.01L; RZPDo839H0993D nucleoporin 133kDa (NUP133) gene, encodes complete protein.
AB384686 - Synthetic construct DNA, clone: pF1KB0166, Homo sapiens NUP133 gene for nucleoporin 133kDa, complete cds, without stop codon, in Flexi system.
MA620073 - JP 2018138019-A/191999: Polycomb-Associated Non-Coding RNAs.
AK001754 - Homo sapiens cDNA FLJ10892 fis, clone NT2RP4002298.
AF052123 - Homo sapiens clone 23770 mRNA sequence.
AK022572 - Homo sapiens cDNA FLJ12510 fis, clone NT2RM2001723.
JD467207 - Sequence 448231 from Patent EP1572962.
JD459969 - Sequence 440993 from Patent EP1572962.
JD197904 - Sequence 178928 from Patent EP1572962.
JD256065 - Sequence 237089 from Patent EP1572962.
JD492839 - Sequence 473863 from Patent EP1572962.
LF375228 - JP 2014500723-A/182731: Polycomb-Associated Non-Coding RNAs.
LF375229 - JP 2014500723-A/182732: Polycomb-Associated Non-Coding RNAs.
AK022047 - Homo sapiens cDNA FLJ11985 fis, clone HEMBB1001356.
LF375232 - JP 2014500723-A/182735: Polycomb-Associated Non-Coding RNAs.
LF375233 - JP 2014500723-A/182736: Polycomb-Associated Non-Coding RNAs.
LF375234 - JP 2014500723-A/182737: Polycomb-Associated Non-Coding RNAs.
LF375235 - JP 2014500723-A/182738: Polycomb-Associated Non-Coding RNAs.
LF375236 - JP 2014500723-A/182739: Polycomb-Associated Non-Coding RNAs.
LF375237 - JP 2014500723-A/182740: Polycomb-Associated Non-Coding RNAs.
LF375241 - JP 2014500723-A/182744: Polycomb-Associated Non-Coding RNAs.
MA610805 - JP 2018138019-A/182731: Polycomb-Associated Non-Coding RNAs.
MA610806 - JP 2018138019-A/182732: Polycomb-Associated Non-Coding RNAs.
MA610809 - JP 2018138019-A/182735: Polycomb-Associated Non-Coding RNAs.
MA610810 - JP 2018138019-A/182736: Polycomb-Associated Non-Coding RNAs.
MA610811 - JP 2018138019-A/182737: Polycomb-Associated Non-Coding RNAs.
MA610812 - JP 2018138019-A/182738: Polycomb-Associated Non-Coding RNAs.
MA610813 - JP 2018138019-A/182739: Polycomb-Associated Non-Coding RNAs.
MA610814 - JP 2018138019-A/182740: Polycomb-Associated Non-Coding RNAs.
MA610818 - JP 2018138019-A/182744: Polycomb-Associated Non-Coding RNAs.
JD180519 - Sequence 161543 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8WUM0 (Reactome details) participates in the following event(s):

R-HSA-75096 Docking of the TAP:EJC Complex with the NPC
R-HSA-165043 Rev multimer-bound HIV mRNA:Crm1:Ran:GTP complex associates with the NPC
R-HSA-180622 Vpr binds nucleoporins
R-HSA-180710 Rev:importin beta:B23 recruited to the nuclear pore
R-HSA-2990882 CDK1 phosphorylates NUP98
R-HSA-75098 mRNP complex dissociates from cytosolic face of NPC
R-HSA-165047 Translocation of nuclear RNA transport complex to cytoplasm
R-HSA-180732 Translocation of Rev:importin-beta:B23 to the nucleus
R-HSA-2990880 NEK6/NEK7 phosphorylates NUP98
R-HSA-141409 Mad1 binds kinetochore
R-HSA-375302 Kinetochore capture of astral microtubules
R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores
R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2
R-HSA-170796 NPC transports GCK1:GKRP from cytosol to nucleoplasm
R-HSA-5578744 Importin-8 imports AGO2:miRNA into the nucleus
R-HSA-6783483 tRNA:XPOT:RAN:GTP translocates from the nucleus to the cytosol
R-HSA-3000348 RANBP2 SUMOylates SP100 with SUMO2
R-HSA-3000399 RANBP2 SUMOylates SP100 with SUMO1
R-HSA-3000411 RANBP2 SUMOylates PML with SUMO2
R-HSA-4570493 RANBP2 (NUP358) SUMOylates HNRNPC with SUMO1
R-HSA-4615872 RANBP2 SUMOylates HDAC4 with SUMO1
R-HSA-4615987 RANBP2 SUMOylates HDAC4 with SUMO2,3
R-HSA-5228508 RANBP2 SUMOylates PML with SUMO1
R-HSA-5228525 RANBP2 SUMOylates TOP2A with SUMO1
R-HSA-192627 Viral mRNA Export
R-HSA-192925 Export of Spliced Viral mRNA
R-HSA-5252041 NPC transports Hikeshi:HSP70s:ATP from cytosol to nucleoplasm
R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex
R-HSA-141439 Release of activated MAD2 from kinetochores
R-HSA-2467811 Separation of sister chromatids
R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin
R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores
R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1
R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin
R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres
R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres
R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-165054 Rev-mediated nuclear export of HIV RNA
R-HSA-180910 Vpr-mediated nuclear import of PICs
R-HSA-180746 Nuclear import of Rev protein
R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly
R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-68877 Mitotic Prometaphase
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-72202 Transport of Mature Transcript to Cytoplasm
R-HSA-162599 Late Phase of HIV Life Cycle
R-HSA-177243 Interactions of Rev with host cellular proteins
R-HSA-176033 Interactions of Vpr with host cellular proteins
R-HSA-2980766 Nuclear Envelope Breakdown
R-HSA-170822 Regulation of Glucokinase by Glucokinase Regulatory Protein
R-HSA-168276 NS1 Mediated Effects on Host Pathways
R-HSA-1169408 ISG15 antiviral mechanism
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-6784531 tRNA processing in the nucleus
R-HSA-3108214 SUMOylation of DNA damage response and repair proteins
R-HSA-4570464 SUMOylation of RNA binding proteins
R-HSA-4551638 SUMOylation of chromatin organization proteins
R-HSA-4615885 SUMOylation of DNA replication proteins
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-3371453 Regulation of HSF1-mediated heat shock response
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-141424 Amplification of signal from the kinetochores
R-HSA-68886 M Phase
R-HSA-195258 RHO GTPase Effectors
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-162587 HIV Life Cycle
R-HSA-162909 Host Interactions of HIV factors
R-HSA-68875 Mitotic Prophase
R-HSA-70171 Glycolysis
R-HSA-168253 Host Interactions with Influenza Factors
R-HSA-1169410 Antiviral mechanism by IFN-stimulated genes
R-HSA-211000 Gene Silencing by RNA
R-HSA-72306 tRNA processing
R-HSA-3108232 SUMO E3 ligases SUMOylate target proteins
R-HSA-168273 Influenza Viral RNA Transcription and Replication
R-HSA-3371556 Cellular response to heat stress
R-HSA-68882 Mitotic Anaphase
R-HSA-69618 Mitotic Spindle Checkpoint
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-194315 Signaling by Rho GTPases
R-HSA-8953854 Metabolism of RNA
R-HSA-162906 HIV Infection
R-HSA-70326 Glucose metabolism
R-HSA-168254 Influenza Infection
R-HSA-913531 Interferon Signaling
R-HSA-74160 Gene expression (Transcription)
R-HSA-2990846 SUMOylation
R-HSA-168255 Influenza Life Cycle
R-HSA-2262752 Cellular responses to stress
R-HSA-2555396 Mitotic Metaphase and Anaphase
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-1640170 Cell Cycle
R-HSA-162582 Signal Transduction
R-HSA-5663205 Infectious disease
R-HSA-71387 Metabolism of carbohydrates
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-597592 Post-translational protein modification
R-HSA-8953897 Cellular responses to external stimuli
R-HSA-1643685 Disease
R-HSA-1430728 Metabolism
R-HSA-168256 Immune System
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: B2RAZ8, NM_018230, NP_060700, NU133_HUMAN, Q5T8N0, Q8WUM0, Q9H9W2, Q9NV71, Q9NVC4
UCSC ID: uc001htn.3
RefSeq Accession: NM_018230
Protein: Q8WUM0 (aka NU133_HUMAN or N133_HUMAN)
CCDS: CCDS1579.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: NM_018230.2
exon count: 26CDS single in 3' UTR: no RNA size: 4170
ORF size: 3471CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 7088.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.