Human Gene PURA (uc003lfa.3) Description and Page Index
Description: Homo sapiens purine-rich element binding protein A (PURA), mRNA. RefSeq Summary (NM_005859): This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC036087.1, DRR138524.233110.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## Transcript (Including UTRs) Position: hg19 chr5:139,493,708-139,499,001 Size: 5,294 Total Exon Count: 1 Strand: + Coding Region Position: hg19 chr5:139,493,767-139,494,735 Size: 969 Coding Exon Count: 1
ID:PURA_HUMAN DESCRIPTION: RecName: Full=Transcriptional activator protein Pur-alpha; AltName: Full=Purine-rich single-stranded DNA-binding protein alpha; FUNCTION: This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination. SUBUNIT: Homodimer, heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1 (By similarity). INTERACTION: P06400:RB1; NbExp=6; IntAct=EBI-1045860, EBI-491274; SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the PUR DNA-binding protein family.
Genetic Association Studies of Complex Diseases and Disorders
Genetic Association Database (archive): PURA CDC HuGE Published Literature: PURA
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF04845 - PurA ssDNA and RNA-binding protein
ModBase Predicted Comparative 3D Structure on Q00577
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0000900 translation repressor activity, nucleic acid binding GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding GO:0003677 DNA binding GO:0003690 double-stranded DNA binding GO:0003691 double-stranded telomeric DNA binding GO:0003697 single-stranded DNA binding GO:0003700 transcription factor activity, sequence-specific DNA binding GO:0003705 transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding GO:0003723 RNA binding GO:0005515 protein binding GO:0008134 transcription factor binding GO:0032422 purine-rich negative regulatory element binding GO:0046332 SMAD binding
Biological Process: GO:0006268 DNA unwinding involved in DNA replication GO:0006270 DNA replication initiation GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006357 regulation of transcription from RNA polymerase II promoter GO:0007399 nervous system development GO:0008284 positive regulation of cell proliferation GO:0017148 negative regulation of translation GO:0030154 cell differentiation GO:0042127 regulation of cell proliferation GO:0045892 negative regulation of transcription, DNA-templated
M96684 - H.sapiens Pur (pur-alpha) mRNA, complete cds. BC110574 - Homo sapiens purine-rich element binding protein A, mRNA (cDNA clone IMAGE:40004320), partial cds. BC110594 - Homo sapiens purine-rich element binding protein A, mRNA (cDNA clone IMAGE:40016155), partial cds. BC036087 - Homo sapiens purine-rich element binding protein A, mRNA (cDNA clone MGC:33782 IMAGE:5284976), complete cds. AB464679 - Synthetic construct DNA, clone: pF1KB9641, Homo sapiens PURA gene for purine-rich element binding protein A, without stop codon, in Flexi system. BT019388 - Homo sapiens purine-rich element binding protein A mRNA, complete cds. JD495994 - Sequence 477018 from Patent EP1572962. X91648 - H.sapiens mRNA for pur alpha extended 3'untranslated region. JD050254 - Sequence 31278 from Patent EP1572962.