Human Gene MED1 (uc002hrv.4) Description and Page Index
  Description: Homo sapiens mediator complex subunit 1 (MED1), mRNA.
RefSeq Summary (NM_004774): The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y13467.1, BC131783.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000300651.11/ ENSP00000300651.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##
Transcript (Including UTRs)
   Position: hg19 chr17:37,560,538-37,607,527 Size: 46,990 Total Exon Count: 17 Strand: -
Coding Region
   Position: hg19 chr17:37,563,728-37,607,315 Size: 43,588 Coding Exon Count: 17 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:37,560,538-37,607,527)mRNA (may differ from genome)Protein (1581 aa)
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Wikipedia

-  Comments and Description Text from UniProtKB
  ID: MED1_HUMAN
DESCRIPTION: RecName: Full=Mediator of RNA polymerase II transcription subunit 1; AltName: Full=Activator-recruited cofactor 205 kDa component; Short=ARC205; AltName: Full=Mediator complex subunit 1; AltName: Full=Peroxisome proliferator-activated receptor-binding protein; Short=PBP; Short=PPAR-binding protein; AltName: Full=Thyroid hormone receptor-associated protein complex 220 kDa component; Short=Trap220; AltName: Full=Thyroid receptor-interacting protein 2; Short=TR-interacting protein 2; Short=TRIP-2; AltName: Full=Vitamin D receptor-interacting protein complex component DRIP205; AltName: Full=p53 regulatory protein RB18A;
FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
SUBUNIT: Interacts with GATA1 and YWHAH (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. This subunit specifically interacts with a number of nuclear receptors in a ligand-dependent fashion including AR, ESR1, ESR2, PPARA, PPARG, RXRA, RXRG, THRA, THRB and VDR. Interacts with CTNNB1, GABPA, GLI3, PPARGC1A and TP53. Binds DNA.
SUBCELLULAR LOCATION: Nucleus. Note=A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
TISSUE SPECIFICITY: Ubiquitously expressed.
PTM: Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the Mediator complex subunit 1 family.
SEQUENCE CAUTION: Sequence=AAC39854.1; Type=Frameshift; Positions=543, 545; Sequence=AAH06517.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence; Sequence=CAA73867.1; Type=Frameshift; Positions=4;

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): MED1
CDC HuGE Published Literature: MED1

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 12.94 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 220.64 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -87.70212-0.414 Picture PostScript Text
3' UTR -964.693190-0.302 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR019680 - Mediator_Med1_met/fun

Pfam Domains:
PF10744 - Mediator of RNA polymerase II transcription subunit 1

Protein Data Bank (PDB) 3-D Structure
MuPIT help

1RJK
- X-ray

1RK3
- X-ray

1RKG
- X-ray
To conserve bandwidth, only the images from the first 3 structures are shown.
1RKH - X-ray 2O4J - X-ray 2O4R - X-ray
2ZFX - X-ray 3A2H - X-ray 3AUN - X-ray
3VJS - X-ray 3VJT - X-ray 3VRT - X-ray
3VRU - X-ray 3VRV - X-ray 3VRW - X-ray


ModBase Predicted Comparative 3D Structure on Q15648
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0000979 RNA polymerase II core promoter sequence-specific DNA binding
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0001047 core promoter binding
GO:0001104 RNA polymerase II transcription cofactor activity
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003712 transcription cofactor activity
GO:0003713 transcription coactivator activity
GO:0005515 protein binding
GO:0008134 transcription factor binding
GO:0016922 ligand-dependent nuclear receptor binding
GO:0030331 estrogen receptor binding
GO:0030374 ligand-dependent nuclear receptor transcription coactivator activity
GO:0030375 thyroid hormone receptor coactivator activity
GO:0031490 chromatin DNA binding
GO:0035257 nuclear hormone receptor binding
GO:0036033 mediator complex binding
GO:0038023 signaling receptor activity
GO:0042809 vitamin D receptor binding
GO:0042974 retinoic acid receptor binding
GO:0042975 peroxisome proliferator activated receptor binding
GO:0044877 macromolecular complex binding
GO:0046966 thyroid hormone receptor binding
GO:0050693 LBD domain binding
GO:0061630 ubiquitin protein ligase activity

Biological Process:
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0000902 cell morphogenesis
GO:0001525 angiogenesis
GO:0001701 in utero embryonic development
GO:0001889 liver development
GO:0001892 embryonic placenta development
GO:0002088 lens development in camera-type eye
GO:0002154 thyroid hormone mediated signaling pathway
GO:0003222 ventricular trabecula myocardium morphogenesis
GO:0003406 retinal pigment epithelium development
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006356 regulation of transcription from RNA polymerase I promoter
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0006590 thyroid hormone generation
GO:0006702 androgen biosynthetic process
GO:0007275 multicellular organism development
GO:0007420 brain development
GO:0007507 heart development
GO:0007595 lactation
GO:0008284 positive regulation of cell proliferation
GO:0009887 animal organ morphogenesis
GO:0010628 positive regulation of gene expression
GO:0010839 negative regulation of keratinocyte proliferation
GO:0016567 protein ubiquitination
GO:0019216 regulation of lipid metabolic process
GO:0030216 keratinocyte differentiation
GO:0030224 monocyte differentiation
GO:0030518 intracellular steroid hormone receptor signaling pathway
GO:0030521 androgen receptor signaling pathway
GO:0031100 animal organ regeneration
GO:0033148 positive regulation of intracellular estrogen receptor signaling pathway
GO:0033160 positive regulation of protein import into nucleus, translocation
GO:0033598 mammary gland epithelial cell proliferation
GO:0033601 positive regulation of mammary gland epithelial cell proliferation
GO:0035050 embryonic heart tube development
GO:0035116 embryonic hindlimb morphogenesis
GO:0035162 embryonic hemopoiesis
GO:0035357 peroxisome proliferator activated receptor signaling pathway
GO:0035729 cellular response to hepatocyte growth factor stimulus
GO:0035855 megakaryocyte development
GO:0042789 mRNA transcription from RNA polymerase II promoter
GO:0043010 camera-type eye development
GO:0043066 negative regulation of apoptotic process
GO:0045444 fat cell differentiation
GO:0045618 positive regulation of keratinocyte differentiation
GO:0045648 positive regulation of erythrocyte differentiation
GO:0045665 negative regulation of neuron differentiation
GO:0045893 positive regulation of transcription, DNA-templated
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048821 erythrocyte development
GO:0048822 enucleate erythrocyte development
GO:0051726 regulation of cell cycle
GO:0060335 positive regulation of interferon-gamma-mediated signaling pathway
GO:0060744 mammary gland branching involved in thelarche
GO:0060745 mammary gland branching involved in pregnancy
GO:0060750 epithelial cell proliferation involved in mammary gland duct elongation
GO:0070318 positive regulation of G0 to G1 transition
GO:0070371 ERK1 and ERK2 cascade
GO:0070562 regulation of vitamin D receptor signaling pathway
GO:0071364 cellular response to epidermal growth factor stimulus
GO:0071383 cellular response to steroid hormone stimulus
GO:0097067 cellular response to thyroid hormone stimulus
GO:2000273 positive regulation of receptor activity
GO:2000347 positive regulation of hepatocyte proliferation
GO:2001141 regulation of RNA biosynthetic process

Cellular Component:
GO:0000151 ubiquitin ligase complex
GO:0000785 chromatin
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0016020 membrane
GO:0016592 mediator complex
GO:0032993 protein-DNA complex


-  Descriptions from all associated GenBank mRNAs
  LF209306 - JP 2014500723-A/16809: Polycomb-Associated Non-Coding RNAs.
LF384800 - JP 2014500723-A/192303: Polycomb-Associated Non-Coding RNAs.
BC060758 - Homo sapiens mediator complex subunit 1, mRNA (cDNA clone IMAGE:4822636), complete cds.
LF327600 - JP 2014500723-A/135103: Polycomb-Associated Non-Coding RNAs.
LF327601 - JP 2014500723-A/135104: Polycomb-Associated Non-Coding RNAs.
LF327602 - JP 2014500723-A/135105: Polycomb-Associated Non-Coding RNAs.
JF432429 - Synthetic construct Homo sapiens clone IMAGE:100073636 mediator complex subunit 1 (MED1) gene, encodes complete protein.
KJ901655 - Synthetic construct Homo sapiens clone ccsbBroadEn_11049 MED1 gene, encodes complete protein.
LF327603 - JP 2014500723-A/135106: Polycomb-Associated Non-Coding RNAs.
LF327604 - JP 2014500723-A/135107: Polycomb-Associated Non-Coding RNAs.
LF327605 - JP 2014500723-A/135108: Polycomb-Associated Non-Coding RNAs.
Y13467 - Homo sapiens mRNA for RB18A protein.
BC131783 - Homo sapiens mediator complex subunit 1, mRNA (cDNA clone MGC:156289 IMAGE:40125129), complete cds.
JD266621 - Sequence 247645 from Patent EP1572962.
JD528456 - Sequence 509480 from Patent EP1572962.
JD215699 - Sequence 196723 from Patent EP1572962.
JD280744 - Sequence 261768 from Patent EP1572962.
JD485867 - Sequence 466891 from Patent EP1572962.
JD329426 - Sequence 310450 from Patent EP1572962.
JD110043 - Sequence 91067 from Patent EP1572962.
AF055994 - Homo sapiens thyroid hormone receptor-associated protein complex component TRAP220 mRNA, complete cds.
AF283812 - Homo sapiens vitamin D receptor-interacting protein complex component DRIP205 (DRIP205) mRNA, complete cds.
AK314185 - Homo sapiens cDNA, FLJ94908, highly similar to Homo sapiens PPAR binding protein (PPARBP), mRNA.
AK299789 - Homo sapiens cDNA FLJ50196 partial cds, highly similar to Peroxisome proliferator-activated receptor-binding protein.
LF327606 - JP 2014500723-A/135109: Polycomb-Associated Non-Coding RNAs.
LF327607 - JP 2014500723-A/135110: Polycomb-Associated Non-Coding RNAs.
LF327608 - JP 2014500723-A/135111: Polycomb-Associated Non-Coding RNAs.
LF327609 - JP 2014500723-A/135112: Polycomb-Associated Non-Coding RNAs.
LF327610 - JP 2014500723-A/135113: Polycomb-Associated Non-Coding RNAs.
LF327611 - JP 2014500723-A/135114: Polycomb-Associated Non-Coding RNAs.
LF327612 - JP 2014500723-A/135115: Polycomb-Associated Non-Coding RNAs.
LF327613 - JP 2014500723-A/135116: Polycomb-Associated Non-Coding RNAs.
LF327614 - JP 2014500723-A/135117: Polycomb-Associated Non-Coding RNAs.
LF327615 - JP 2014500723-A/135118: Polycomb-Associated Non-Coding RNAs.
LF327616 - JP 2014500723-A/135119: Polycomb-Associated Non-Coding RNAs.
BC006517 - Homo sapiens mediator complex subunit 1, mRNA (cDNA clone IMAGE:3546031), partial cds.
LF327617 - JP 2014500723-A/135120: Polycomb-Associated Non-Coding RNAs.
L40366 - Homo sapiens thyroid receptor interactor (TRIP2) mRNA, partial cds.
LF327618 - JP 2014500723-A/135121: Polycomb-Associated Non-Coding RNAs.
LF327619 - JP 2014500723-A/135122: Polycomb-Associated Non-Coding RNAs.
DQ581522 - Homo sapiens piRNA piR-49634, complete sequence.
LF327620 - JP 2014500723-A/135123: Polycomb-Associated Non-Coding RNAs.
LF327621 - JP 2014500723-A/135124: Polycomb-Associated Non-Coding RNAs.
LF327622 - JP 2014500723-A/135125: Polycomb-Associated Non-Coding RNAs.
CU687400 - Synthetic construct Homo sapiens gateway clone IMAGE:100021184 5' read MED1 mRNA.
LF327625 - JP 2014500723-A/135128: Polycomb-Associated Non-Coding RNAs.
LF327626 - JP 2014500723-A/135129: Polycomb-Associated Non-Coding RNAs.
LF327629 - JP 2014500723-A/135132: Polycomb-Associated Non-Coding RNAs.
LF327630 - JP 2014500723-A/135133: Polycomb-Associated Non-Coding RNAs.
JD427196 - Sequence 408220 from Patent EP1572962.
JD395293 - Sequence 376317 from Patent EP1572962.
MA620377 - JP 2018138019-A/192303: Polycomb-Associated Non-Coding RNAs.
MA563177 - JP 2018138019-A/135103: Polycomb-Associated Non-Coding RNAs.
MA563178 - JP 2018138019-A/135104: Polycomb-Associated Non-Coding RNAs.
MA563179 - JP 2018138019-A/135105: Polycomb-Associated Non-Coding RNAs.
MA563180 - JP 2018138019-A/135106: Polycomb-Associated Non-Coding RNAs.
MA563181 - JP 2018138019-A/135107: Polycomb-Associated Non-Coding RNAs.
MA563182 - JP 2018138019-A/135108: Polycomb-Associated Non-Coding RNAs.
MA563183 - JP 2018138019-A/135109: Polycomb-Associated Non-Coding RNAs.
MA563184 - JP 2018138019-A/135110: Polycomb-Associated Non-Coding RNAs.
MA563185 - JP 2018138019-A/135111: Polycomb-Associated Non-Coding RNAs.
MA563186 - JP 2018138019-A/135112: Polycomb-Associated Non-Coding RNAs.
MA563187 - JP 2018138019-A/135113: Polycomb-Associated Non-Coding RNAs.
MA563188 - JP 2018138019-A/135114: Polycomb-Associated Non-Coding RNAs.
MA563189 - JP 2018138019-A/135115: Polycomb-Associated Non-Coding RNAs.
MA563190 - JP 2018138019-A/135116: Polycomb-Associated Non-Coding RNAs.
MA563191 - JP 2018138019-A/135117: Polycomb-Associated Non-Coding RNAs.
MA563192 - JP 2018138019-A/135118: Polycomb-Associated Non-Coding RNAs.
MA563193 - JP 2018138019-A/135119: Polycomb-Associated Non-Coding RNAs.
MA563194 - JP 2018138019-A/135120: Polycomb-Associated Non-Coding RNAs.
MA563195 - JP 2018138019-A/135121: Polycomb-Associated Non-Coding RNAs.
MA563196 - JP 2018138019-A/135122: Polycomb-Associated Non-Coding RNAs.
MA563197 - JP 2018138019-A/135123: Polycomb-Associated Non-Coding RNAs.
MA563198 - JP 2018138019-A/135124: Polycomb-Associated Non-Coding RNAs.
MA563199 - JP 2018138019-A/135125: Polycomb-Associated Non-Coding RNAs.
MA563202 - JP 2018138019-A/135128: Polycomb-Associated Non-Coding RNAs.
MA563203 - JP 2018138019-A/135129: Polycomb-Associated Non-Coding RNAs.
MA563206 - JP 2018138019-A/135132: Polycomb-Associated Non-Coding RNAs.
MA563207 - JP 2018138019-A/135133: Polycomb-Associated Non-Coding RNAs.
MA444883 - JP 2018138019-A/16809: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q15648 (Reactome details) participates in the following event(s):

R-HSA-212352 Formation of ARC coactivator complex
R-HSA-212380 Formation of TRAP coactivator complex
R-HSA-212432 Formation of DRIP coactivator complex
R-HSA-376419 Formation of NR-MED1 Coactivator Complex
R-HSA-9023840 HATs and coactivators are recruited to the CTSD gene
R-HSA-381309 PPARG:RXRA heterodimer binds to fatty acid-like ligands
R-HSA-400143 Fatty acid ligands activate PPARA
R-HSA-212436 Generic Transcription Pathway
R-HSA-383280 Nuclear Receptor transcription pathway
R-HSA-9018519 Estrogen-dependent gene expression
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
R-HSA-400206 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-8939211 ESR-mediated signaling
R-HSA-1266738 Developmental Biology
R-HSA-556833 Metabolism of lipids
R-HSA-74160 Gene expression (Transcription)
R-HSA-9006931 Signaling by Nuclear Receptors
R-HSA-1430728 Metabolism
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: A2RRQ6, ARC205, CRSP1, CRSP200, DRIP205, DRIP230, MED1_HUMAN, NM_004774, NP_004765, O43810, O75447, PBP, PPARBP, PPARGBP, Q15648, Q6P9H7, Q6PK58, Q9HD39, RB18A, TRAP220, TRIP2
UCSC ID: uc002hrv.4
RefSeq Accession: NM_004774
Protein: Q15648 (aka MED1_HUMAN)
CCDS: CCDS11336.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_004774.3
exon count: 17CDS single in 3' UTR: no RNA size: 8148
ORF size: 4746CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 9151.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: yes # AT/AC introns 0
selenocysteine: no end bleed into intron: 6296# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.