Human Gene DPP8 (uc002aox.3) Description and Page Index
  Description: Homo sapiens dipeptidyl-peptidase 8 (DPP8), transcript variant 1, mRNA.
RefSeq Summary (NM_130434): This gene encodes a member of the peptidase S9B family, a small family of dipeptidyl peptidases that are able to cleave peptide substrates at a prolyl bond. The encoded protein shares similarity with dipeptidyl peptidase IV in that it is ubiquitously expressed, and hydrolyzes the same substrates. These similarities suggest that, like dipeptidyl peptidase IV, this protein may play a role in T-cell activation and immune function. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
   Position: hg19 chr15:65,737,998-65,810,035 Size: 72,038 Total Exon Count: 20 Strand: -
Coding Region
   Position: hg19 chr15:65,739,222-65,804,891 Size: 65,670 Coding Exon Count: 19 

Page IndexSequence and LinksUniProtKB CommentsCTDGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr15:65,737,998-65,810,035)mRNA (may differ from genome)Protein (882 aa)
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H-INVHGNCHPRDLynxMGIneXtProt
OMIMPubMedStanford SOURCETreefamUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: DPP8_HUMAN
DESCRIPTION: RecName: Full=Dipeptidyl peptidase 8; Short=DP8; EC=3.4.14.5; AltName: Full=Dipeptidyl peptidase IV-related protein 1; Short=DPRP-1; AltName: Full=Dipeptidyl peptidase VIII; Short=DPP VIII; AltName: Full=Prolyl dipeptidase DPP8;
FUNCTION: Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. May play a role in T-cell activation and immune function.
CATALYTIC ACTIVITY: Release of an N-terminal dipeptide, Xaa-Yaa-|- Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
ENZYME REGULATION: Inhibited by zinc. Inhibited by the serine proteinase inhibitor 4-(2-aminoethyl)benzenesulphonyl fluoride (AEBSF), and by di-isopropylfuorophosphate. Specifically inhibited by isoindoline derivatives.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=208 uM for Ala-Pro-AMC; KM=130 uM for Ala-Pro-AFC; KM=120 uM for H-Ala-Pro-pNa; KM=1420 uM for H-Ala-Ala-pNa; KM=310 uM for H-Arg-Pro-pNa; KM=2050 uM for H-Asp-Pro-pNa; KM=480 uM for H-Gly-Pro-pNa; pH dependence: Optimum pH is 7.4-8.5. Little activity below pH 6.5;
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Ubiquitously expressed, with highest levels in testis, placenta, prostate, muscle and brain.
INDUCTION: In activated T-cells.
SIMILARITY: Belongs to the peptidase S9B family. DPPIV subfamily.
SEQUENCE CAUTION: Sequence=AAQ13623.1; Type=Frameshift; Positions=Several; Sequence=AAQ13650.1; Type=Frameshift; Positions=Several; Sequence=AAQ13657.1; Type=Erroneous initiation; Sequence=BAA91059.1; Type=Frameshift; Positions=486; Sequence=BAB55395.1; Type=Erroneous initiation;

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 8.24 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 255.59 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -101.40223-0.455 Picture PostScript Text
3' UTR -277.501224-0.227 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001375 - Peptidase_S9
IPR002469 - Peptidase_S9B

Pfam Domains:
PF00326 - Prolyl oligopeptidase family
PF00930 - Dipeptidyl peptidase IV (DPP IV) N-terminal region
PF02129 - X-Pro dipeptidyl-peptidase (S15 family)

SCOP Domains:
82171 - Dipeptidyl peptidase IV/CD26, N-terminal domain
53474 - alpha/beta-Hydrolases

ModBase Predicted Comparative 3D Structure on Q6V1X1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004177 aminopeptidase activity
GO:0008233 peptidase activity
GO:0008236 serine-type peptidase activity
GO:0008239 dipeptidyl-peptidase activity
GO:0016787 hydrolase activity

Biological Process:
GO:0006508 proteolysis
GO:0006955 immune response

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  BC040203 - Homo sapiens dipeptidyl-peptidase 8, mRNA (cDNA clone IMAGE:4824813), with apparent retained intron.
AK000290 - Homo sapiens cDNA FLJ20283 fis, clone HEP04088.
AF221634 - Homo sapiens dipeptidyl peptidase 8 (DPP8) mRNA, complete cds.
BC030688 - Homo sapiens dipeptidyl-peptidase 8, mRNA (cDNA clone MGC:26191 IMAGE:4822550), complete cds.
AK291057 - Homo sapiens cDNA FLJ77467 complete cds, highly similar to Homo sapiens dipeptidylpeptidase 8 (DPP8), transcript variant 3, mRNA.
AY354202 - Homo sapiens dipeptidyl peptidase 8 isoform 3 (DPP8) mRNA, complete cds, alternatively spliced.
AK302638 - Homo sapiens cDNA FLJ60979 complete cds, highly similar to Dipeptidyl peptidase 8 (EC 3.4.14.5).
AK296915 - Homo sapiens cDNA FLJ56045 complete cds, highly similar to Dipeptidyl peptidase 8 (EC 3.4.14.5).
DQ891733 - Synthetic construct clone IMAGE:100004363; FLH179504.01X; RZPDo839H03130D dipeptidyl-peptidase 8 (DPP8) gene, encodes complete protein.
DQ894910 - Synthetic construct Homo sapiens clone IMAGE:100009370; FLH179500.01L; RZPDo839H03129D dipeptidyl-peptidase 8 (DPP8) gene, encodes complete protein.
KJ900817 - Synthetic construct Homo sapiens clone ccsbBroadEn_10211 DPP8 gene, encodes complete protein.
AY172659 - Homo sapiens dipeptidyl peptidase IV-related protein-1 (DPRP1) mRNA, complete cds.
CU688984 - Synthetic construct Homo sapiens gateway clone IMAGE:100020498 5' read DPP8 mRNA.
AF173382 - Homo sapiens MSTP097 (MST097) mRNA, complete cds.
AF176779 - Homo sapiens MSTP141 mRNA, complete cds.
AF221635 - Homo sapiens dipeptidyl peptidase 8 (DPP8) mRNA, partial cds, alternatively spliced.
AF221636 - Homo sapiens dipeptidyl peptidase 8 (DPP8) mRNA, partial cds, alternatively spliced.
AF175225 - Homo sapiens tissue-type aorta MSTP135 mRNA, complete cds.
AK027826 - Homo sapiens cDNA FLJ14920 fis, clone PLACE1007416, weakly similar to DIPEPTIDYL PEPTIDASE IV (EC 3.4.14.5).
JD047064 - Sequence 28088 from Patent EP1572962.
JD091896 - Sequence 72920 from Patent EP1572962.
AF221637 - Homo sapiens dipeptidyl peptidase 8 (DPP8) mRNA, partial cds, alternatively spliced.
JD468741 - Sequence 449765 from Patent EP1572962.
JD073796 - Sequence 54820 from Patent EP1572962.
JD380994 - Sequence 362018 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: DPP8_HUMAN, DPRP1, MSTP097, MSTP135, MSTP141, NM_130434, NP_569118, Q6V1X1, Q7Z4C8, Q7Z4D3, Q7Z4E1, Q8IWG7, Q8NEM5, Q96JX1, Q9HBM2, Q9HBM3, Q9HBM4, Q9HBM5, Q9NXF4
UCSC ID: uc002aox.3
RefSeq Accession: NM_130434
Protein: Q6V1X1 (aka DPP8_HUMAN)
CCDS: CCDS10207.1, CCDS10210.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_130434.3
exon count: 20CDS single in 3' UTR: no RNA size: 4096
ORF size: 2649CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 5464.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.