Description: Homo sapiens endothelial differentiation-related factor 1 (EDF1), transcript variant alpha, mRNA. RefSeq Summary (NM_003792): This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]. Transcript (Including UTRs) Position: hg19 chr9:139,756,571-139,760,738 Size: 4,168 Total Exon Count: 5 Strand: - Coding Region Position: hg19 chr9:139,756,736-139,760,710 Size: 3,975 Coding Exon Count: 5
ID:EDF1_HUMAN DESCRIPTION: RecName: Full=Endothelial differentiation-related factor 1; Short=EDF-1; AltName: Full=Multiprotein-bridging factor 1; Short=MBF1; FUNCTION: Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. SUBUNIT: Interacts with TBP and the transcription factor IID (TFIID) complex, NR5A2, NR1H3 and PPARG. Interaction with TBP is regulated by phosphorylation. Binds NR5A1, ATF1, FOS and JUN via their conserved basic region. Binding to calmodulin is regulated by calcium and phosphorylation of the IQ motif. INTERACTION: Q13133:NR1H3; NbExp=4; IntAct=EBI-781301, EBI-781356; Q04752:NR5A1 (xeno); NbExp=4; IntAct=EBI-781310, EBI-850837; O00482:NR5A2; NbExp=2; IntAct=EBI-781301, EBI-781320; P37231:PPARG; NbExp=4; IntAct=EBI-781301, EBI-781384; P20226:TBP; NbExp=2; IntAct=EBI-781310, EBI-355371; SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin. TISSUE SPECIFICITY: Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues. DEVELOPMENTAL STAGE: Expressed in fetal tissues. More abundant in kidney. INDUCTION: Down-regulated by HIV-1 Tat or phorbol ester (TPA) treatment in endothelial cells (at mRNA and protein levels). DOMAIN: The IQ motif, which is involved in calmodulin binding, overlaps with the binding domain for nuclear receptors and transcription factors. Its phosphorylation probably allows a switch between the two activities of the protein (By similarity). PTM: Phosphorylated (by PKA and PKC). SIMILARITY: Contains 1 HTH cro/C1-type DNA-binding domain. SEQUENCE CAUTION: Sequence=CAI12698.1; Type=Erroneous gene model prediction;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O60869
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
US Server
