Description: Homo sapiens ectonucleoside triphosphate diphosphohydrolase 4 (ENTPD4), transcript variant 1, mRNA. RefSeq Summary (NM_004901): This gene encodes a member of the apyrase protein family. Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. The encoded protein is an endo-apyrase and may play a role in salvaging nucleotides from lysosomes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and these isoforms may differ in divalent cation dependence and substrate specificity. [provided by RefSeq, Sep 2011]. Transcript (Including UTRs) Position: hg19 chr8:23,286,665-23,315,244 Size: 28,580 Total Exon Count: 13 Strand: - Coding Region Position: hg19 chr8:23,290,439-23,307,413 Size: 16,975 Coding Exon Count: 12
ID:ENTP4_HUMAN DESCRIPTION: RecName: Full=Ectonucleoside triphosphate diphosphohydrolase 4; Short=NTPDase 4; EC=3.6.1.6; AltName: Full=Lysosomal apyrase-like protein of 70 kDa; AltName: Full=Uridine-diphosphatase; Short=UDPase; FUNCTION: Hydrolyzes preferentially nucleoside 5'-diphosphates, nucleoside 5'-triphosphates are hydrolyzed only to a minor extent. The order of activity with different substrates is UDP >> GDP = CDP = TDP, AMP, ADP, ATP and UMP are not substrates. Preferred substrates for isoform 2 are CTP, UDP, CDP, GTP and GDP, while isoform 1 utilizes UTP and TTP. CATALYTIC ACTIVITY: A nucleoside diphosphate + H(2)O = a nucleotide + phosphate. COFACTOR: Calcium. COFACTOR: Isoform 1 requires calcium and to a lesser extent, magnesium. Isoform 2 is equally dependent on calcium and magnesium. SUBCELLULAR LOCATION: Golgi apparatus membrane; Multi-pass membrane protein (Potential). Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein (Potential). Note=Localizes in the Golgi and autophagic vacuoles/lysosomes. TISSUE SPECIFICITY: Ubiquitous. Highest expression in testis and lowest in bladder. SIMILARITY: Belongs to the GDA1/CD39 NTPase family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF01150 - GDA1/CD39 (nucleoside phosphatase) family
ModBase Predicted Comparative 3D Structure on Q9Y227
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.