Human Gene CCT4 (uc002sbo.4) Description and Page Index
  Description: Homo sapiens chaperonin containing TCP1, subunit 4 (delta) (CCT4), transcript variant 1, mRNA.
RefSeq Summary (NM_006430): The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]).[supplied by OMIM, Mar 2008].
Transcript (Including UTRs)
   Position: hg19 chr2:62,095,262-62,115,806 Size: 20,545 Total Exon Count: 14 Strand: -
Coding Region
   Position: hg19 chr2:62,095,827-62,115,642 Size: 19,816 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsMalaCardsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr2:62,095,262-62,115,806)mRNA (may differ from genome)Protein (539 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
neXtProtOMIMPubMedReactomeStanford SOURCEUniProtKB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=T-complex protein 1 subunit delta; Short=TCP-1-delta; AltName: Full=CCT-delta; AltName: Full=Stimulator of TAR RNA-binding;
FUNCTION: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
SUBUNIT: Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Component of the BBS/CCT complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8.
SUBCELLULAR LOCATION: Cytoplasm. Melanosome. Cytoplasm, cytoskeleton, centrosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: Belongs to the TCP-1 chaperonin family.

-  MalaCards Disease Associations
  MalaCards Gene Search: CCT4
Diseases sorted by gene-association score: mental retardation, x-linked, snyder-robinson type (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 105.20 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 2258.23 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -61.80164-0.377 Picture PostScript Text
3' UTR -127.40565-0.225 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR012717 - Chap_CCT_delta
IPR017998 - Chaperone_TCP-1
IPR002194 - Chaperonin_TCP-1_CS
IPR002423 - Cpn60/TCP-1

Pfam Domains:
PF00118 - TCP-1/cpn60 chaperonin family

SCOP Domains:
48592 - GroEL equatorial domain-like
52029 - GroEL apical domain-like
54849 - GroEL-intermediate domain like

ModBase Predicted Comparative 3D Structure on P50991
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserGenome BrowserGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene SorterGene Sorter
 Protein SequenceProtein SequenceProtein SequenceProtein SequenceProtein Sequence

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0044183 protein binding involved in protein folding
GO:0051082 unfolded protein binding

Biological Process:
GO:0006457 protein folding
GO:0006458 'de novo' protein folding
GO:0007339 binding of sperm to zona pellucida
GO:0032212 positive regulation of telomere maintenance via telomerase
GO:0050821 protein stabilization
GO:0051973 positive regulation of telomerase activity
GO:0061077 chaperone-mediated protein folding
GO:0090666 scaRNA localization to Cajal body
GO:1901998 toxin transport
GO:1904851 positive regulation of establishment of protein localization to telomere
GO:1904871 positive regulation of protein localization to Cajal body
GO:1904874 positive regulation of telomerase RNA localization to Cajal body

Cellular Component:
GO:0002199 zona pellucida receptor complex
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005813 centrosome
GO:0005815 microtubule organizing center
GO:0005829 cytosol
GO:0005832 chaperonin-containing T-complex
GO:0005856 cytoskeleton
GO:0005874 microtubule
GO:0042470 melanosome
GO:0042995 cell projection
GO:0044297 cell body
GO:0070062 extracellular exosome

-  Descriptions from all associated GenBank mRNAs
  AF026291 - Homo sapiens chaperonin containing t-complex polypeptide 1, delta subunit (Cctd) mRNA, complete cds.
U38846 - Human stimulator of TAR RNA binding (SRB) mRNA, complete cds.
AK129860 - Homo sapiens cDNA FLJ26350 fis, clone HRT04717, highly similar to T-complex protein 1, delta subunit.
BC014676 - Homo sapiens, clone IMAGE:4158571, mRNA, partial cds.
AK290538 - Homo sapiens cDNA FLJ77660 complete cds.
AK303082 - Homo sapiens cDNA FLJ51167 complete cds, highly similar to T-complex protein 1 subunit delta.
AK295330 - Homo sapiens cDNA FLJ50963 complete cds, highly similar to T-complex protein 1 subunit delta.
JD164629 - Sequence 145653 from Patent EP1572962.
BC106933 - Homo sapiens chaperonin containing TCP1, subunit 4 (delta), mRNA (cDNA clone MGC:126164 IMAGE:40033761), complete cds.
BC106934 - Homo sapiens chaperonin containing TCP1, subunit 4 (delta), mRNA (cDNA clone MGC:126165 IMAGE:40033762), complete cds.
AK294665 - Homo sapiens cDNA FLJ53873 complete cds, highly similar to T-complex protein 1 subunit delta.
AK315975 - Homo sapiens cDNA, FLJ78874 complete cds, highly similar to T-complex protein 1 subunit delta.
AK308942 - Homo sapiens cDNA, FLJ98983.
AK312586 - Homo sapiens cDNA, FLJ92961, highly similar to Homo sapiens chaperonin containing TCP1, subunit 4 (delta) (CCT4),mRNA.
AK307856 - Homo sapiens cDNA, FLJ97804.
EU446763 - Synthetic construct Homo sapiens clone IMAGE:100069901; IMAGE:100011972; FLH257057.01L chaperonin containing TCP1, subunit 4 (delta) (CCT4) gene, encodes complete protein.
KJ893080 - Synthetic construct Homo sapiens clone ccsbBroadEn_02474 CCT4 gene, encodes complete protein.
AB385091 - Synthetic construct DNA, clone: pF1KB5383, Homo sapiens CCT4 gene for T-complex protein 1 subunit delta, complete cds, without stop codon, in Flexi system.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_bcellsurvivalPathway - B Cell Survival Pathway

Reactome (by CSHL, EBI, and GO)

Protein P50991 (Reactome details) participates in the following event(s):

R-HSA-389954 Hydrolysis of ATP and release of tubulin folding intermediate from CCT/TriC
R-HSA-389961 ADP is exchanged for ATP in the (ADP:CCT/TriC):tubulin complex
R-HSA-389970 Actin/tubulin:prefoldin complex associates with CCT/TriC
R-HSA-390470 Association of CCT/TriC with other substrates during biosynthesis (unknown chaperone)
R-HSA-391266 Association of CCT/TriC with sphingosine kinase 1
R-HSA-6814119 TRiC/CCT binds unfolded G-protein beta subunit
R-HSA-390453 Hydrolysis of ATP and release of folded actin from CCT/TriC
R-HSA-8850527 PDCL releases folded G-beta from TRiC/CCT
R-HSA-8850539 Release of GNB5:RGS dimers from CCT/TRiC
R-HSA-390459 Exchange of ADP for ATP in CCT/TriC:actin complex
R-HSA-6814124 ATP binds G-protein beta associated TRiC/CCT
R-HSA-8850529 RGS proteins bind GNB5 and CCT/TRiC
R-HSA-8850534 PDCL promotes G-protein beta 5 folding
R-HSA-6814121 PDCL binds G-protein beta and TRiC/CCT
R-HSA-6814120 ATP hydrolysis promotes folding of G-protein beta by TRiC/CCT
R-HSA-389960 Formation of tubulin folding intermediates by CCT/TriC
R-HSA-389957 Prefoldin mediated transfer of substrate to CCT/TriC
R-HSA-390471 Association of TriC/CCT with target proteins during biosynthesis
R-HSA-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding
R-HSA-390450 Folding of actin by CCT/TriC
R-HSA-389958 Cooperation of Prefoldin and TriC/CCT in actin and tubulin folding
R-HSA-390466 Chaperonin-mediated protein folding
R-HSA-391251 Protein folding
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: B2R6I3, CCTD, NM_006430, NP_006421, O14870, P50991, Q53QP9, Q96C51, SRB, TCPD_HUMAN, uc002sbo.3
UCSC ID: uc002sbo.4
RefSeq Accession: NM_006430
Protein: P50991 (aka TCPD_HUMAN)
CCDS: CCDS33206.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: NM_006430.3
exon count: 14CDS single in 3' UTR: no RNA size: 2352
ORF size: 1620CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3313.00frame shift in genome: no % Coverage: 99.87
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
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-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.