Human Gene FBXO5 (uc003qpg.3) Description and Page Index
Description: Homo sapiens F-box protein 5 (FBXO5), transcript variant 1, mRNA. RefSeq Summary (NM_012177): This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Dec 2008]. Transcript (Including UTRs) Position: hg19 chr6:153,291,658-153,304,203 Size: 12,546 Total Exon Count: 5 Strand: - Coding Region Position: hg19 chr6:153,292,298-153,304,094 Size: 11,797 Coding Exon Count: 5
ID:FBX5_HUMAN DESCRIPTION: RecName: Full=F-box only protein 5; AltName: Full=Early mitotic inhibitor 1; FUNCTION: Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding. SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity). Interacts with BTRC, FZR1/CDH1 and the N-terminal substrate-binding domain of CDC20. Also interacts with EVI5 which blocks its phosphorylation by PLK1 and prevents its subsequent binding to BTRC and degradation. INTERACTION: P30260:CDC27; NbExp=2; IntAct=EBI-852298, EBI-994813; O60447:EVI5; NbExp=6; IntAct=EBI-852298, EBI-852291; SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Note=In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle. DEVELOPMENTAL STAGE: Accumulates in late G1 phase, levels rise during S phase and drop in early mitosis. DOMAIN: The C-terminal region is required for inhibition of APC activity. PTM: Phosphorylation by CDK2 and subsequently by PLK1 triggers degradation during early mitosis through ubiquitin-mediated proteolysis by the SCF ubiquitin ligase complex containing the F- box protein BTRC. This degradation is necessary for the activation of APC in late mitosis and subsequent mitotic progression. PTM: Ubiquitinated by the by the SCF(BTRC) complex following phosphorylation by PLK1. SIMILARITY: Contains 1 F-box domain. SIMILARITY: Contains 1 IBR-type zinc finger.
Genetic Association Studies of Complex Diseases and Disorders
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9UKT4
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.