Human Gene CENPK (uc003jtu.3) Description and Page Index
  Description: Homo sapiens centromere protein K (CENPK), transcript variant 2, mRNA.
RefSeq Summary (NM_001267038): CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM, Mar 2008].
Transcript (Including UTRs)
   Position: hg19 chr5:64,813,593-64,858,995 Size: 45,403 Total Exon Count: 11 Strand: -
Coding Region
   Position: hg19 chr5:64,814,302-64,850,734 Size: 36,433 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsMalaCardsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr5:64,813,593-64,858,995)mRNA (may differ from genome)Protein (269 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
OMIMPubMedReactomeStanford SOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Centromere protein K; Short=CENP-K; AltName: Full=Interphase centromere complex protein 37; AltName: Full=Protein AF-5alpha; AltName: Full=p33;
FUNCTION: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore.
SUBUNIT: Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts directly with CENPH.
SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex.
TISSUE SPECIFICITY: Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta.
DISEASE: Note=Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with MLL.
SIMILARITY: Belongs to the CENPK family.

-  MalaCards Disease Associations
  MalaCards Gene Search: CENPK
Diseases sorted by gene-association score: vertebrobasilar insufficiency (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.82 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 30.52 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -54.94178-0.309 Picture PostScript Text
3' UTR -133.12709-0.188 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR020993 - Centromere_CenpK

Pfam Domains:
PF11802 - Centromere-associated protein K

ModBase Predicted Comparative 3D Structure on Q9BS16
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserNo orthologNo orthologNo ortholog
  Protein Sequence   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0034080 CENP-A containing nucleosome assembly
GO:0051382 kinetochore assembly

Cellular Component:
GO:0000775 chromosome, centromeric region
GO:0000776 kinetochore
GO:0000777 condensed chromosome kinetochore
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005694 chromosome
GO:0005829 cytosol

-  Descriptions from all associated GenBank mRNAs
  AY033646 - Homo sapiens leucine zipper protein AF5alpha mRNA, complete cds.
BC008504 - Homo sapiens centromere protein K, mRNA (cDNA clone MGC:14847 IMAGE:3511065), complete cds.
BC005400 - Homo sapiens centromere protein K, mRNA (cDNA clone MGC:12540 IMAGE:3839409), complete cds.
AF315941 - Homo sapiens P33 mRNA, complete cds.
JD264111 - Sequence 245135 from Patent EP1572962.
JD326124 - Sequence 307148 from Patent EP1572962.
JD238868 - Sequence 219892 from Patent EP1572962.
JD117807 - Sequence 98831 from Patent EP1572962.
JD348193 - Sequence 329217 from Patent EP1572962.
AY009151 - Homo sapiens leucine zipper protein FKSG14 (FKSG14) mRNA, complete cds.
CU675159 - Synthetic construct Homo sapiens gateway clone IMAGE:100018677 5' read CENPK mRNA.
DQ892541 - Synthetic construct clone IMAGE:100005171; FLH187313.01X; RZPDo839F1272D centromere protein K (CENPK) gene, encodes complete protein.
KJ894530 - Synthetic construct Homo sapiens clone ccsbBroadEn_03924 CENPK gene, encodes complete protein.
DQ895752 - Synthetic construct Homo sapiens clone IMAGE:100010212; FLH187309.01L; RZPDo839F1262D centromere protein K (CENPK) gene, encodes complete protein.
AB528341 - Synthetic construct DNA, clone: pF1KB6369, Homo sapiens CENPK gene for centromere protein K, without stop codon, in Flexi system.
JD089304 - Sequence 70328 from Patent EP1572962.
JD106368 - Sequence 87392 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9BS16 (Reactome details) participates in the following event(s):

R-HSA-606349 Mis18 complex binds the centromere
R-HSA-141409 Mad1 binds kinetochore
R-HSA-375302 Kinetochore capture of astral microtubules
R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores
R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2
R-HSA-606326 HJURP:CENPA complex localizes to the centromere
R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex
R-HSA-141439 Release of activated MAD2 from kinetochores
R-HSA-2467811 Separation of sister chromatids
R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin
R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores
R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1
R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin
R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres
R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres
R-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-68877 Mitotic Prometaphase
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-774815 Nucleosome assembly
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-141424 Amplification of signal from the kinetochores
R-HSA-68886 M Phase
R-HSA-195258 RHO GTPase Effectors
R-HSA-73886 Chromosome Maintenance
R-HSA-68882 Mitotic Anaphase
R-HSA-69618 Mitotic Spindle Checkpoint
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-194315 Signaling by Rho GTPases
R-HSA-1640170 Cell Cycle
R-HSA-2555396 Mitotic Metaphase and Anaphase
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: CENPK_HUMAN, FKSG14, ICEN37, NM_001267038, NP_071428, Q9BS16, Q9H4L0
UCSC ID: uc003jtu.3
RefSeq Accession: NM_001267038
Protein: Q9BS16 (aka CENPK_HUMAN)
CCDS: CCDS3984.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: NM_001267038.1
exon count: 11CDS single in 3' UTR: no RNA size: 1719
ORF size: 810CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1650.50frame shift in genome: no % Coverage: 98.72
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.