Human Gene UBE2F (uc031rrz.1) Description and Page Index
  Description: Homo sapiens ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 2, mRNA.
Transcript (Including UTRs)
   Position: hg19 chr2:238,877,435-238,951,423 Size: 73,989 Total Exon Count: 10 Strand: +
Coding Region
   Position: hg19 chr2:238,881,750-238,949,979 Size: 68,230 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsCTDGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:238,877,435-238,951,423)mRNA (may differ from genome)Protein (185 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsH-INV
HGNCHPRDLynxMGIneXtProtOMIM
PubMedReactomeStanford SOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: UBE2F_HUMAN
DESCRIPTION: RecName: Full=NEDD8-conjugating enzyme UBE2F; EC=6.3.2.-; AltName: Full=NEDD8 carrier protein UBE2F; AltName: Full=NEDD8 protein ligase UBE2F; AltName: Full=NEDD8-conjugating enzyme 2; AltName: Full=Ubiquitin-conjugating enzyme E2 F;
FUNCTION: Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
CATALYTIC ACTIVITY: ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.
PATHWAY: Protein modification; protein neddylation.
SUBUNIT: Interacts with UBA3 and RBX2.
TISSUE SPECIFICITY: Widely expressed (at protein level).
SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 4.82 RPKM in Heart - Atrial Appendage
Total median expression: 155.08 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -32.60133-0.245 Picture PostScript Text
3' UTR -426.201444-0.295 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000608 - UBQ-conjugat_E2
IPR023313 - UBQ-conjugating_AS
IPR016135 - UBQ-conjugating_enzyme/RWD

Pfam Domains:
PF00179 - Ubiquitin-conjugating enzyme

SCOP Domains:
54495 - UBC-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help

2EDI
- NMR MuPIT

3FN1
- X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q969M7
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details  Gene Details 
Gene SorterGene Sorter  Gene Sorter 
 RGDEnsembl WormBase 
 Protein SequenceProtein Sequence Protein Sequence 
 AlignmentAlignment Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016740 transferase activity
GO:0019788 NEDD8 transferase activity
GO:0031625 ubiquitin protein ligase binding
GO:0061630 ubiquitin protein ligase activity
GO:0061654 NEDD8 conjugating enzyme activity

Biological Process:
GO:0016567 protein ubiquitination
GO:0043687 post-translational protein modification
GO:0045116 protein neddylation

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AK293334 - Homo sapiens cDNA FLJ58839 complete cds, highly similar to Homo sapiens NEDD8-conjugating enzyme (NCE2), mRNA.
AK297502 - Homo sapiens cDNA FLJ60703 complete cds, highly similar to Homo sapiens NEDD8-conjugating enzyme (NCE2), mRNA.
AK002119 - Homo sapiens cDNA FLJ11257 fis, clone PLACE1009020, weakly similar to NIFS PROTEIN.
AK303094 - Homo sapiens cDNA FLJ61005 complete cds, highly similar to Homo sapiens NEDD8-conjugating enzyme (NCE2), mRNA.
AK290063 - Homo sapiens cDNA FLJ76590 complete cds, highly similar to Homo sapiens NEDD8-conjugating enzyme (NCE2), mRNA.
BC010549 - Homo sapiens ubiquitin-conjugating enzyme E2F (putative), mRNA (cDNA clone MGC:18120 IMAGE:4153270), complete cds.
AK294107 - Homo sapiens cDNA FLJ60473 complete cds, moderately similar to Homo sapiens NEDD8-conjugating enzyme (NCE2), mRNA.
AF310723 - Homo sapiens NEDD8-conjugating enzyme NCE2 (NCE2) mRNA, complete cds.
JF432618 - Synthetic construct Homo sapiens clone IMAGE:100073855 ubiquitin-conjugating enzyme E2F (putative) (UBE2F) gene, encodes complete protein.
BC039435 - Homo sapiens cDNA clone IMAGE:5303613.
JD152493 - Sequence 133517 from Patent EP1572962.
JD067243 - Sequence 48267 from Patent EP1572962.
DQ582932 - Homo sapiens piRNA piR-50044, complete sequence.
JD287409 - Sequence 268433 from Patent EP1572962.
JD280102 - Sequence 261126 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04120 - Ubiquitin mediated proteolysis

BioCarta from NCI Cancer Genome Anatomy Project
h_parkinPathway - Role of Parkin in the Ubiquitin-Proteasomal Pathway

Reactome (by CSHL, EBI, and GO)

Protein Q969M7 (Reactome details) participates in the following event(s):

R-HSA-8951766 Doubly neddylated UBA3:NAE1 binds AcM-UBE2F
R-HSA-8952044 AcM-UBE2F transfers NEDD8 to CRL5 E3 ubiquitin ligase complex
R-HSA-983152 Transfer of ubiquitin from E1 to E2
R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2
R-HSA-8951764 Transfer of NEDD8 to AcM-UBE2F
R-HSA-8952039 NEDD8:AcM-UBE2F binds CRL5 E3 ubiquitin ligase complex
R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex
R-HSA-8951664 Neddylation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-HSA-597592 Post-translational protein modification
R-HSA-983169 Class I MHC mediated antigen processing & presentation
R-HSA-392499 Metabolism of proteins
R-HSA-1280218 Adaptive Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: A8K1Z8, B4DDT9, B4DFI1, B4DMK3, B4DZU2, B8ZZG2, NCE2, NM_001278305, NP_001265234, Q969M7, UBE2F_HUMAN, uc002vxk.3
UCSC ID: uc031rrz.1
RefSeq Accession: NM_001278305
Protein: Q969M7 (aka UBE2F_HUMAN)
CCDS: CCDS2523.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001278305.1
exon count: 10CDS single in 3' UTR: no RNA size: 2135
ORF size: 558CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1304.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.