Human Gene WASH1 (uc002cfg.1) Description and Page Index
  Description: Homo sapiens WAS protein family homolog 1 (WASH1), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr16:66,541-69,448 Size: 2,908 Total Exon Count: 6 Strand: -
Coding Region
   Position: hg19 chr16:66,541-68,789 Size: 2,249 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsCTDGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:66,541-69,448)mRNA (may differ from genome)Protein (306 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaCGAP
EnsemblEntrez GeneExonPrimerGeneCardsHGNCLynx
MGIneXtProtOMIMPubMedStanford SOURCETreefam
UniProtKB

-  Comments and Description Text from UniProtKB
  ID: WASH4_HUMAN
DESCRIPTION: RecName: Full=Putative WAS protein family homolog 4; AltName: Full=Protein FAM39CP;
FUNCTION: Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (By similarity).
SUBUNIT: Component of the WASH complex, composed of F-actin- capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin- capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. Interacts (via WHD1 region) with FAM21C; the interaction is direct (By similarity).
SUBCELLULAR LOCATION: Early endosome membrane (By similarity). Recycling endosome membrane (By similarity). Note=Localization to the endodome membrane is mediated via its interaction with FAM21 (By similarity).
MISCELLANEOUS: WASH genes duplicated to multiple chromosomal ends during primate evolution, with highest copy number reached in humans, whose WASH repertoires probably vary extensively among individuals (PubMed:18159949). It is therefore difficult to determine which gene is functional or not. The telomeric region of chromosome 9p is paralogous to the pericentromeric regions of chromosome 9 as well as to 2q. Paralogous regions contain 7 transcriptional units. Duplicated WASH genes are also present in the Xq/Yq pseudoautosomal region, as well as on chromosome 1 and 15. The chromosome 16 copy seems to be a pseudogene.
SIMILARITY: Belongs to the WASH1 family.
SIMILARITY: Contains 1 WH2 domain.
CAUTION: Could be the product of a pseudogene.

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 2.88 RPKM in Prostate
Total median expression: 74.37 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -239.31659-0.363 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR021854 - WASH_WASD

Pfam Domains:
PF11945 - WAHD domain of WASH complex

ModBase Predicted Comparative 3D Structure on A8MWX3
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003779 actin binding
GO:0043014 alpha-tubulin binding

Biological Process:
GO:0016197 endosomal transport
GO:0034314 Arp2/3 complex-mediated actin nucleation
GO:0042147 retrograde transport, endosome to Golgi

Cellular Component:
GO:0005768 endosome
GO:0005769 early endosome
GO:0005829 cytosol
GO:0016020 membrane
GO:0031901 early endosome membrane
GO:0055037 recycling endosome
GO:0055038 recycling endosome membrane
GO:0071203 WASH complex


-  Descriptions from all associated GenBank mRNAs
  BC064371 - Homo sapiens cDNA clone IMAGE:5588748, partial cds.
BC093083 - Homo sapiens WAS protein family homolog 2 pseudogene, mRNA (cDNA clone IMAGE:30388014).
JD446251 - Sequence 427275 from Patent EP1572962.
JD114876 - Sequence 95900 from Patent EP1572962.
JD270338 - Sequence 251362 from Patent EP1572962.
JD392218 - Sequence 373242 from Patent EP1572962.
JD520988 - Sequence 502012 from Patent EP1572962.
JD216409 - Sequence 197433 from Patent EP1572962.
JD164536 - Sequence 145560 from Patent EP1572962.
JD145347 - Sequence 126371 from Patent EP1572962.
JD493632 - Sequence 474656 from Patent EP1572962.
JD295896 - Sequence 276920 from Patent EP1572962.
JD273037 - Sequence 254061 from Patent EP1572962.
JD267962 - Sequence 248986 from Patent EP1572962.
JD090362 - Sequence 71386 from Patent EP1572962.
JD090361 - Sequence 71385 from Patent EP1572962.
JD338175 - Sequence 319199 from Patent EP1572962.
JD544421 - Sequence 525445 from Patent EP1572962.
JD283907 - Sequence 264931 from Patent EP1572962.
JD405727 - Sequence 386751 from Patent EP1572962.
JD405726 - Sequence 386750 from Patent EP1572962.
JD408497 - Sequence 389521 from Patent EP1572962.
JD127621 - Sequence 108645 from Patent EP1572962.
JD099061 - Sequence 80085 from Patent EP1572962.
JD461476 - Sequence 442500 from Patent EP1572962.
JD170368 - Sequence 151392 from Patent EP1572962.
JD519949 - Sequence 500973 from Patent EP1572962.
JD042751 - Sequence 23775 from Patent EP1572962.
JD098685 - Sequence 79709 from Patent EP1572962.
JD151500 - Sequence 132524 from Patent EP1572962.
JD540282 - Sequence 521306 from Patent EP1572962.
JD442700 - Sequence 423724 from Patent EP1572962.
JD370682 - Sequence 351706 from Patent EP1572962.
JD178750 - Sequence 159774 from Patent EP1572962.
JD211783 - Sequence 192807 from Patent EP1572962.
JD408352 - Sequence 389376 from Patent EP1572962.
JD347215 - Sequence 328239 from Patent EP1572962.
JD200549 - Sequence 181573 from Patent EP1572962.
JD466474 - Sequence 447498 from Patent EP1572962.
JD523450 - Sequence 504474 from Patent EP1572962.
JD360523 - Sequence 341547 from Patent EP1572962.
JD412153 - Sequence 393177 from Patent EP1572962.
JD523449 - Sequence 504473 from Patent EP1572962.
JD115240 - Sequence 96264 from Patent EP1572962.
JD252028 - Sequence 233052 from Patent EP1572962.
JD148890 - Sequence 129914 from Patent EP1572962.
JD080383 - Sequence 61407 from Patent EP1572962.
JD477709 - Sequence 458733 from Patent EP1572962.
JD548722 - Sequence 529746 from Patent EP1572962.
JD050182 - Sequence 31206 from Patent EP1572962.
JD392712 - Sequence 373736 from Patent EP1572962.
JD172379 - Sequence 153403 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A8MWX3, AY217347, FAM39CP, NM_182905, NP_878908, WASH4P, WASH4_HUMAN
UCSC ID: uc002cfg.1
Representative RNA: AY217347
Protein: A8MWX3 (aka WASH4_HUMAN)

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: AY217347.1
exon count: 6CDS single in 3' UTR: no RNA size: 2447
ORF size: 918CDS single in intron: no Alignment % ID: 98.32
txCdsPredict score: 1442.50frame shift in genome: no % Coverage: 64.41
has start codon: yes stop codon in genome: no # of Alignments: 7
has end codon: no retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.