Human Gene DCTN2 (uc001som.2) Description and Page Index
  Description: Homo sapiens dynactin 2 (p50) (DCTN2), transcript variant 3, mRNA.
RefSeq Summary (NM_006400): This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012].
Transcript (Including UTRs)
   Position: hg19 chr12:57,923,833-57,941,114 Size: 17,282 Total Exon Count: 14 Strand: -
Coding Region
   Position: hg19 chr12:57,924,472-57,940,846 Size: 16,375 Coding Exon Count: 14 

Page IndexSequence and LinksMalaCardsCTDGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesmRNA DescriptionsPathways
Other NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:57,923,833-57,941,114)mRNA (may differ from genome)Protein (406 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMGI
OMIMPubMedReactomeStanford SOURCEUniProtKBWikipedia

-  MalaCards Disease Associations
  MalaCards Gene Search: DCTN2
Diseases sorted by gene-association score: retinitis pigmentosa (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 66.22 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 2452.27 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -100.84268-0.376 Picture PostScript Text
3' UTR -214.56639-0.336 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  Pfam Domains:
PF04912 - Dynamitin

ModBase Predicted Comparative 3D Structure on Q13561-2
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
 Protein Sequence    
 Alignment    

-  Descriptions from all associated GenBank mRNAs
  BC064331 - Homo sapiens cDNA clone IMAGE:5797909, **** WARNING: chimeric clone ****.
AK096242 - Homo sapiens cDNA FLJ38923 fis, clone NT2NE2011823, highly similar to Dynactin subunit 2.
BC009468 - Homo sapiens dynactin 2 (p50), mRNA (cDNA clone MGC:16056 IMAGE:3613836), complete cds.
FJ224315 - Homo sapiens epididymis secretory protein Li 77 (HEL-S-77) mRNA, complete cds.
U50733 - Homo sapiens dynamitin mRNA, complete cds.
BC014083 - Homo sapiens dynactin 2 (p50), mRNA (cDNA clone MGC:20331 IMAGE:4303142), complete cds.
AK098794 - Homo sapiens cDNA FLJ25928 fis, clone CBR05333, highly similar to DYNACTIN, 50 KDA ISOFORM.
BC000718 - Homo sapiens dynactin 2 (p50), mRNA (cDNA clone MGC:1336 IMAGE:3504109), complete cds.
GQ891494 - Homo sapiens clone HEL-S-215 epididymis secretory sperm binding protein mRNA, complete cds.
AK055682 - Homo sapiens cDNA FLJ31120 fis, clone IMR322000730, highly similar to Dynactin subunit 2.
AK222693 - Homo sapiens mRNA for dynactin 2 variant, clone: CBR05685.
AK292364 - Homo sapiens cDNA FLJ77785 complete cds.
AK314705 - Homo sapiens cDNA, FLJ95559, Homo sapiens dynactin 2 (p50) (DCTN2), mRNA.
HQ447201 - Synthetic construct Homo sapiens clone IMAGE:100070498; CCSB003453_01 dynactin 2 (p50) (DCTN2) gene, encodes complete protein.
KJ902126 - Synthetic construct Homo sapiens clone ccsbBroadEn_11520 DCTN2 gene, encodes complete protein.
AB590544 - Synthetic construct DNA, clone: pFN21AE1882, Homo sapiens DCTN2 gene for dynactin 2, without stop codon, in Flexi system.
AY189155 - Homo sapiens p50 dynamitin mRNA, complete cds.
CU674356 - Synthetic construct Homo sapiens gateway clone IMAGE:100018226 5' read DCTN2 mRNA.
JD295925 - Sequence 276949 from Patent EP1572962.
JD324665 - Sequence 305689 from Patent EP1572962.
JD307519 - Sequence 288543 from Patent EP1572962.
JD551860 - Sequence 532884 from Patent EP1572962.
JD175004 - Sequence 156028 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04962 - Vasopressin-regulated water reabsorption
hsa05016 - Huntington's disease

Reactome (by CSHL, EBI, and GO)

Protein Q13561 (Reactome details) participates in the following event(s):

R-HSA-380272 Plk1-mediated phosphorylation of Nlp
R-HSA-380283 Recruitment of additional gamma tubulin/ gamma TuRC to the centrosome
R-HSA-380294 Loss of C-Nap-1 from centrosomes
R-HSA-380311 Recruitment of Plk1 to centrosomes
R-HSA-380455 Recruitment of CDK11p58 to the centrosomes
R-HSA-380303 Dissociation of Phospho-Nlp from the centrosome
R-HSA-5626220 C2CD3 binds the mother centriole
R-HSA-380508 Translocation of NuMA to the centrosomes
R-HSA-2574845 AJUBA binds centrosome-associated AURKA
R-HSA-8853405 TPX2 binds AURKA at centrosomes
R-HSA-3000319 BORA binds PLK1 and AURKA
R-HSA-2574840 AJUBA facilitates AURKA autophosphorylation
R-HSA-3000310 AURKA phosphorylates PLK1
R-HSA-5626223 C2CD3 and OFD1 recruit 5 distal appendage proteins to the centriole
R-HSA-5626681 Recruitment of transition zone proteins
R-HSA-5626227 CP110 and CEP97 dissociate from the centriole
R-HSA-380316 Association of NuMA with microtubules
R-HSA-8853419 TPX2 promotes AURKA autophosphorylation
R-HSA-5626228 The distal appendage proteins recruit TTBK2
R-HSA-5638009 CEP164 recruits RAB3IP-carrying Golgi-derived vesicles to the basal body
R-HSA-5626699 MARK4 binds ODF2 in the centriole
R-HSA-5617816 RAB3IP stimulates nucleotide exchange on RAB8A
R-HSA-6809003 ERGIC-to-Golgi vesicles bind dynein:dynactin
R-HSA-6809006 Vesicle is tethered through binding GOLGA2:GORASP1, GOLGB1 and the COG complex
R-HSA-8849350 RAB6:GTP displaces PAFAH1B1 from dynein:dynactin complex
R-HSA-380259 Loss of Nlp from mitotic centrosomes
R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes
R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome
R-HSA-5620912 Anchoring of the basal body to the plasma membrane
R-HSA-380320 Recruitment of NuMA to mitotic centrosomes
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-8854518 AURKA Activation by TPX2
R-HSA-380287 Centrosome maturation
R-HSA-5617833 Cilium Assembly
R-HSA-68877 Mitotic Prometaphase
R-HSA-69275 G2/M Transition
R-HSA-1852241 Organelle biogenesis and maintenance
R-HSA-68886 M Phase
R-HSA-453274 Mitotic G2-G2/M phases
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-2132295 MHC class II antigen presentation
R-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-HSA-1640170 Cell Cycle
R-HSA-1280218 Adaptive Immune System
R-HSA-199977 ER to Golgi Anterograde Transport
R-HSA-8856688 Golgi-to-ER retrograde transport
R-HSA-168256 Immune System
R-HSA-199991 Membrane Trafficking
R-HSA-948021 Transport to the Golgi and subsequent modification
R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic
R-HSA-5653656 Vesicle-mediated transport
R-HSA-446203 Asparagine N-linked glycosylation
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: DCTN50, NM_006400, NP_001248342, Q13561-2, uc001som.1
UCSC ID: uc001som.2
RefSeq Accession: NM_006400
Protein: Q13561-2, splice isoform of Q13561 CCDS: CCDS44930.1, CCDS58245.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_006400.4
exon count: 14CDS single in 3' UTR: no RNA size: 2128
ORF size: 1206CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 2612.00frame shift in genome: no % Coverage: 99.30
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.