Human Gene FBXO3 (uc001muz.3) Description and Page Index
  Description: Homo sapiens F-box protein 3 (FBXO3), transcript variant 1, mRNA.
RefSeq Summary (NM_012175): This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants diverging at the 3' end. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
   Position: hg19 chr11:33,762,490-33,796,071 Size: 33,582 Total Exon Count: 11 Strand: -
Coding Region
   Position: hg19 chr11:33,763,454-33,796,043 Size: 32,590 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr11:33,762,490-33,796,071)mRNA (may differ from genome)Protein (471 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
neXtProtOMIMPubMedStanford SOURCETreefamUniProtKB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=F-box only protein 3;
FUNCTION: Substrate recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53- dependent transactivation.
SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO3, SKP1, CUL1 and RBX1. Interacts with PML, interaction is direct and takes place either alone or within the SCF complex.
INTERACTION: P04608:tat (xeno); NbExp=3; IntAct=EBI-2509901, EBI-6164389;
SUBCELLULAR LOCATION: Nucleus. Note=Colocalizes with PML at the peripheries of nuclear bodies.
SIMILARITY: Contains 1 apaG domain.
SIMILARITY: Contains 1 F-box domain.

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): FBXO3
CDC HuGE Published Literature: FBXO3
Positive Disease Associations: Interleukin-6 , Monocytes , Parkinson Disease
Related Studies:
  1. Interleukin-6
    Emelia J Benjamin et al. BMC medical genetics 2007, Genome-wide association with select biomarker traits in the Framingham Heart Study., BMC medical genetics. [PubMed 17903293]
    The Framingham GWAS represents a resource to describe potentially novel genetic influences on systemic biomarker variability. The newly described associations will need to be replicated in other studies.
  2. Monocytes
    , , . [PubMed 0]
  3. Monocytes
    , , . [PubMed 0]
           more ... click here to view the complete list

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 8.92 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 257.39 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -8.0028-0.286 Picture PostScript Text
3' UTR -227.20964-0.236 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007474 - ApaG_domain
IPR018958 - Cell_wall_assmbl_KNR4-like
IPR001810 - F-box_dom_cyclin-like

Pfam Domains:
PF00646 - F-box domain
PF04379 - ApaG domain
PF09346 - SMI1 / KNR4 family (SUKH-1)
PF12937 - F-box-like

SCOP Domains:
81383 - F-box domain
110069 - ApaG-like (Pfam 04379)

ModBase Predicted Comparative 3D Structure on Q9UK99
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
  Protein Sequence   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004842 ubiquitin-protein transferase activity
GO:0005515 protein binding

Biological Process:
GO:0006508 proteolysis
GO:0016567 protein ubiquitination

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005829 cytosol

-  Descriptions from all associated GenBank mRNAs
  LF205803 - JP 2014500723-A/13306: Polycomb-Associated Non-Coding RNAs.
AB209039 - Homo sapiens mRNA for F-box only protein 3 isoform 1 variant protein.
BC046110 - Homo sapiens F-box protein 3, mRNA (cDNA clone MGC:57538 IMAGE:5549548), complete cds.
AK001943 - Homo sapiens cDNA FLJ11081 fis, clone PLACE1005187, weakly similar to APAG PROTEIN.
AL136625 - Homo sapiens mRNA; cDNA DKFZp564B092 (from clone DKFZp564B092).
BC039291 - Homo sapiens F-box protein 3, mRNA (cDNA clone IMAGE:5296662), complete cds.
AK123198 - Homo sapiens cDNA FLJ41204 fis, clone BRACE3000404.
JD503283 - Sequence 484307 from Patent EP1572962.
AL832826 - Homo sapiens mRNA; cDNA DKFZp667I1524 (from clone DKFZp667I1524).
AK300721 - Homo sapiens cDNA FLJ58692 complete cds, highly similar to F-box only protein 3.
JD534207 - Sequence 515231 from Patent EP1572962.
JD315899 - Sequence 296923 from Patent EP1572962.
LF348390 - JP 2014500723-A/155893: Polycomb-Associated Non-Coding RNAs.
AK316286 - Homo sapiens cDNA, FLJ79185 complete cds, highly similar to F-box only protein 3.
JD135352 - Sequence 116376 from Patent EP1572962.
LF348389 - JP 2014500723-A/155892: Polycomb-Associated Non-Coding RNAs.
JD467505 - Sequence 448529 from Patent EP1572962.
KJ893554 - Synthetic construct Homo sapiens clone ccsbBroadEn_02948 FBXO3 gene, encodes complete protein.
EU832324 - Synthetic construct Homo sapiens clone HAIB:100067353; DKFZo008E0827 F-box protein 3 protein (FBXO3) gene, encodes complete protein.
EU832409 - Synthetic construct Homo sapiens clone HAIB:100067438; DKFZo004E0828 F-box protein 3 protein (FBXO3) gene, encodes complete protein.
LF348388 - JP 2014500723-A/155891: Polycomb-Associated Non-Coding RNAs.
AK128454 - Homo sapiens cDNA FLJ46597 fis, clone THYMU3046360, highly similar to F-box only protein 3.
AF176702 - Homo sapiens F-box protein FBX3 mRNA, partial cds.
LF348381 - JP 2014500723-A/155884: Polycomb-Associated Non-Coding RNAs.
AK304866 - Homo sapiens cDNA FLJ59154 complete cds, highly similar to F-box only protein 3.
LF348380 - JP 2014500723-A/155883: Polycomb-Associated Non-Coding RNAs.
LF348379 - JP 2014500723-A/155882: Polycomb-Associated Non-Coding RNAs.
CU692298 - Synthetic construct Homo sapiens gateway clone IMAGE:100021494 5' read FBXO3 mRNA.
LF348378 - JP 2014500723-A/155881: Polycomb-Associated Non-Coding RNAs.
AF174595 - Homo sapiens F-box protein Fbx3 (FBX3) mRNA, partial cds.
LF348377 - JP 2014500723-A/155880: Polycomb-Associated Non-Coding RNAs.
LF212906 - JP 2014500723-A/20409: Polycomb-Associated Non-Coding RNAs.
MA583967 - JP 2018138019-A/155893: Polycomb-Associated Non-Coding RNAs.
MA583966 - JP 2018138019-A/155892: Polycomb-Associated Non-Coding RNAs.
MA583965 - JP 2018138019-A/155891: Polycomb-Associated Non-Coding RNAs.
MA583958 - JP 2018138019-A/155884: Polycomb-Associated Non-Coding RNAs.
MA583957 - JP 2018138019-A/155883: Polycomb-Associated Non-Coding RNAs.
MA583956 - JP 2018138019-A/155882: Polycomb-Associated Non-Coding RNAs.
MA583955 - JP 2018138019-A/155881: Polycomb-Associated Non-Coding RNAs.
MA583954 - JP 2018138019-A/155880: Polycomb-Associated Non-Coding RNAs.
MA441380 - JP 2018138019-A/13306: Polycomb-Associated Non-Coding RNAs.
MA448483 - JP 2018138019-A/20409: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: B3KY16, D3DR05, FBX3, FBX3_HUMAN, NM_012175, NP_036307, Q86X90, Q9H0V2, Q9NUX2, Q9UK99
UCSC ID: uc001muz.3
RefSeq Accession: NM_012175
Protein: Q9UK99 (aka FBX3_HUMAN)
CCDS: CCDS7887.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: NM_012175.3
exon count: 11CDS single in 3' UTR: no RNA size: 2408
ORF size: 1416CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3032.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.