Human Gene GPR65 (uc001xvv.3) Description and Page Index
  Description: Homo sapiens G protein-coupled receptor 65 (GPR65), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr14:88,471,468-88,481,155 Size: 9,688 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg19 chr14:88,477,192-88,478,205 Size: 1,014 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr14:88,471,468-88,481,155)mRNA (may differ from genome)Protein (337 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
neXtProtOMIMPubMedReactomeStanford SOURCETreefam

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Psychosine receptor; AltName: Full=G-protein coupled receptor 65; AltName: Full=T-cell death-associated gene 8 protein;
FUNCTION: Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids. May have a role in activation- induced cell death or differentiation of T-cells.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Predominantly expressed in thymus, spleen, lymph nodes, small intestine, lung, placenta and peripheral blood leukocytes.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): GPR65
CDC HuGE Published Literature: GPR65
Positive Disease Associations: Bipolar Disorder , Crohn Disease , gamma-Glutamylcyclotransferase , Multiple Sclerosis , Myocardial Infarction , Prostatic Neoplasms
Related Studies:
  1. Bipolar Disorder
    Yuan Jiang et al. Genetic epidemiology 2011, Propensity score-based nonparametric test revealing genetic variants underlying bipolar disorder., Genetic epidemiology. [PubMed 21254220]
  2. Crohn Disease
    Andre Franke et al. Nature genetics 2010, Genome-wide meta-analysis increases to 71 the number of confirmed Crohn's disease susceptibility loci., Nature genetics. [PubMed 21102463]
  3. gamma-Glutamylcyclotransferase
    Emelia J Benjamin et al. BMC medical genetics 2007, Genome-wide association with select biomarker traits in the Framingham Heart Study., BMC medical genetics. [PubMed 17903293]
    The Framingham GWAS represents a resource to describe potentially novel genetic influences on systemic biomarker variability. The newly described associations will need to be replicated in other studies.
           more ... click here to view the complete list

-  MalaCards Disease Associations
  MalaCards Gene Search: GPR65
Diseases sorted by gene-association score: rectal prolapse (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 12.59 RPKM in Spleen
Total median expression: 70.68 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -115.27558-0.207 Picture PostScript Text
3' UTR -885.062950-0.300 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR005464 - Psych_rcpt

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on Q8IYL9
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
 Gene Details    
 Gene Sorter    
 Protein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity

Biological Process:
GO:0006915 apoptotic process
GO:0006955 immune response
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007189 adenylate cyclase-activating G-protein coupled receptor signaling pathway
GO:0007275 multicellular organism development
GO:0010447 response to acidic pH
GO:0031532 actin cytoskeleton reorganization
GO:0035025 positive regulation of Rho protein signal transduction
GO:0051482 positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway
GO:0051496 positive regulation of stress fiber assembly
GO:0090630 activation of GTPase activity

Cellular Component:
GO:0005622 intracellular
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane

-  Descriptions from all associated GenBank mRNAs
  LQ740459 - Sequence 13 from Patent WO2018083189.
AK313797 - Homo sapiens cDNA, FLJ94412, highly similar to Homo sapiens G protein-coupled receptor 65 (GPR65), mRNA.
BC035633 - Homo sapiens G protein-coupled receptor 65, mRNA (cDNA clone MGC:45304 IMAGE:5218256), complete cds.
BC071715 - Homo sapiens G protein-coupled receptor 65, mRNA (cDNA clone MGC:88010 IMAGE:5180030), complete cds.
DL491772 - Novel nucleic acids.
JD353815 - Sequence 334839 from Patent EP1572962.
JD496255 - Sequence 477279 from Patent EP1572962.
JD516434 - Sequence 497458 from Patent EP1572962.
CU690142 - Synthetic construct Homo sapiens gateway clone IMAGE:100016734 5' read GPR65 mRNA.
DQ893989 - Synthetic construct Homo sapiens clone IMAGE:100008449; FLH166318.01L; RZPDo839F0685D G protein-coupled receptor 65 (GPR65) gene, encodes complete protein.
KJ897850 - Synthetic construct Homo sapiens clone ccsbBroadEn_07244 GPR65 gene, encodes complete protein.
EU883574 - Homo sapiens orphan GPCR 65 (GPR65) mRNA, complete cds.
DQ890834 - Synthetic construct clone IMAGE:100003464; FLH166322.01X; RZPDo839F0686D G protein-coupled receptor 65 (GPR65) gene, encodes complete protein.
JD380890 - Sequence 361914 from Patent EP1572962.
JD564020 - Sequence 545044 from Patent EP1572962.
JD263308 - Sequence 244332 from Patent EP1572962.
JD433975 - Sequence 414999 from Patent EP1572962.
JD312492 - Sequence 293516 from Patent EP1572962.
JD528271 - Sequence 509295 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8IYL9 (Reactome details) participates in the following event(s):

R-HSA-444731 GPR4, GPR65, GPR132 and OGR1 are pH sensing receptors
R-HSA-749452 The Ligand:GPCR:Gq complex dissociates
R-HSA-749448 Liganded Gq-activating GPCRs bind inactive heterotrimeric Gq
R-HSA-379048 Liganded Gq/11-activating GPCRs act as GEFs for Gq/11
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-416476 G alpha (q) signalling events
R-HSA-500792 GPCR ligand binding
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: NM_003608, NP_003599, O75819, PSYR_HUMAN, Q8IYL9, TDAG8
UCSC ID: uc001xvv.3
RefSeq Accession: NM_003608
Protein: Q8IYL9 (aka PSYR_HUMAN)
CCDS: CCDS9879.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: NM_003608.3
exon count: 2CDS single in 3' UTR: no RNA size: 4522
ORF size: 1014CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1979.50frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.