Description: Homo sapiens cleavage and polyadenylation specific factor 4, 30kDa (CPSF4), transcript variant 1, mRNA. RefSeq Summary (NM_006693): Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]. Transcript (Including UTRs) Position: hg19 chr7:99,036,563-99,054,996 Size: 18,434 Total Exon Count: 8 Strand: + Coding Region Position: hg19 chr7:99,036,706-99,054,123 Size: 17,418 Coding Exon Count: 8
ID:CPSF4_HUMAN DESCRIPTION: RecName: Full=Cleavage and polyadenylation specificity factor subunit 4; AltName: Full=Cleavage and polyadenylation specificity factor 30 kDa subunit; Short=CPSF 30 kDa subunit; AltName: Full=NS1 effector domain-binding protein 1; Short=Neb-1; AltName: Full=No arches homolog; FUNCTION: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U). SUBUNIT: Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. Interacts with FIP1L1. Association with influenza A virus NS1 blocks processing of pre-mRNAs, thereby preventing nuclear export of host cell mRNAs. SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the CPSF4/YTH1 family. SIMILARITY: Contains 5 C3H1-type zinc fingers. SIMILARITY: Contains 1 CCHC-type zinc finger. WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/cpsf4/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O95639
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
JC506674 - Sequence 42 from Patent EP2733220. JC737786 - Sequence 42 from Patent WO2014075939. JC506688 - Sequence 56 from Patent EP2733220. JC737800 - Sequence 56 from Patent WO2014075939. BC050738 - Homo sapiens cleavage and polyadenylation specific factor 4, 30kDa, mRNA (cDNA clone MGC:60384 IMAGE:6199123), complete cds. BC003101 - Homo sapiens cleavage and polyadenylation specific factor 4, 30kDa, mRNA (cDNA clone MGC:1870 IMAGE:3356071), complete cds. U79569 - Human no arches (nar) mRNA, complete cds. AB529148 - Synthetic construct DNA, clone: pF1KB5204, Homo sapiens CPSF4 gene for cleavage and polyadenylation specific factor 4, 30kDa, without stop codon, in Flexi system. CU690790 - Synthetic construct Homo sapiens gateway clone IMAGE:100021318 5' read CPSF4 mRNA. HQ448622 - Synthetic construct Homo sapiens clone IMAGE:100072061; CCSB011823_01 cleavage and polyadenylation specific factor 4, 30kDa (CPSF4) gene, encodes complete protein. KJ893157 - Synthetic construct Homo sapiens clone ccsbBroadEn_02551 CPSF4 gene, encodes complete protein. KJ902171 - Synthetic construct Homo sapiens clone ccsbBroadEn_11565 CPSF4 gene, encodes complete protein. CR542161 - Homo sapiens full open reading frame cDNA clone RZPDo834C0424D for gene CPSF4, cleavage and polyadenylation specific factor 4, 30kDa; complete cds, without stopcodon. AK301745 - Homo sapiens cDNA FLJ52791 complete cds, highly similar to Cleavage and polyadenylation specificityfactor 30 kDa subunit. JD210384 - Sequence 191408 from Patent EP1572962. JD229891 - Sequence 210915 from Patent EP1572962. JD103059 - Sequence 84083 from Patent EP1572962. JD247440 - Sequence 228464 from Patent EP1572962. JD227459 - Sequence 208483 from Patent EP1572962. JD399129 - Sequence 380153 from Patent EP1572962. JD131979 - Sequence 113003 from Patent EP1572962. JD343279 - Sequence 324303 from Patent EP1572962. JD384328 - Sequence 365352 from Patent EP1572962. JD209414 - Sequence 190438 from Patent EP1572962. JD519409 - Sequence 500433 from Patent EP1572962. JD392950 - Sequence 373974 from Patent EP1572962. JD230573 - Sequence 211597 from Patent EP1572962. JD515562 - Sequence 496586 from Patent EP1572962. JD178515 - Sequence 159539 from Patent EP1572962. JD224246 - Sequence 205270 from Patent EP1572962. JD332662 - Sequence 313686 from Patent EP1572962. JD051245 - Sequence 32269 from Patent EP1572962. JD161399 - Sequence 142423 from Patent EP1572962. JD150929 - Sequence 131953 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_cpsfPathway - Polyadenylation of mRNA
Reactome (by CSHL, EBI, and GO)
Protein O95639 (Reactome details) participates in the following event(s):
R-HSA-168859 Binding of NS1 to cleavage and host polyadenylation specificity factor (CPSF) R-HSA-72231 Cleavage and Polyadenylation R-HSA-77589 Recognition of AAUAAA sequence by CPSF R-HSA-72185 mRNA polyadenylation R-HSA-77594 Docking of the Mature intronless derived transcript derived mRNA, TAP and Aly/Ref at the NPC R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-72180 Cleavage of mRNA at the 3'-end R-HSA-77590 Recruitment of CstF to the CPSF Bound Pre-mRNA R-HSA-77592 Cleavage of Intronless Pre-mRNA at 3'-end R-HSA-77593 Cleavage and polyadenylation of Intronless Pre-mRNA R-HSA-5696813 TSEN complex cleaves the intron from pre-tRNA R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-8849157 TREX complex binds spliced, capped mRNA:CBC:EJC cotranscriptionally R-HSA-156661 Formation of Exon Junction Complex R-HSA-77591 Binding of Cleavage factors and Poly(A)Polymerase to the CstF:CPSF:Pre-mRNA Complex R-HSA-168315 Inhibition of Host mRNA Processing and RNA Silencing R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-77595 Processing of Intronless Pre-mRNAs R-HSA-72187 mRNA 3'-end processing R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-168276 NS1 Mediated Effects on Host Pathways R-HSA-109688 Cleavage of Growing Transcript in the Termination Region R-HSA-72172 mRNA Splicing R-HSA-75067 Processing of Capped Intronless Pre-mRNA R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-159234 Transport of Mature mRNAs Derived from Intronless Transcripts R-HSA-6784531 tRNA processing in the nucleus R-HSA-168253 Host Interactions with Influenza Factors R-HSA-73856 RNA Polymerase II Transcription Termination R-HSA-8953854 Metabolism of RNA R-HSA-72202 Transport of Mature Transcript to Cytoplasm R-HSA-72306 tRNA processing R-HSA-168254 Influenza Infection R-HSA-73857 RNA Polymerase II Transcription R-HSA-5663205 Infectious disease R-HSA-74160 Gene expression (Transcription) R-HSA-1643685 Disease
US Server
