Human Gene LAP3 (uc003gph.1) Description and Page Index
  Description: Homo sapiens leucine aminopeptidase 3 (LAP3), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr4:17,578,927-17,609,590 Size: 30,664 Total Exon Count: 13 Strand: +
Coding Region
   Position: hg19 chr4:17,579,089-17,609,212 Size: 30,124 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:17,578,927-17,609,590)mRNA (may differ from genome)Protein (519 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
H-INVHGNCHPRDLynxMGIneXtProt
OMIMPubMedStanford SOURCETreefamUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: AMPL_HUMAN
DESCRIPTION: RecName: Full=Cytosol aminopeptidase; EC=3.4.11.1; AltName: Full=Leucine aminopeptidase 3; Short=LAP-3; AltName: Full=Leucyl aminopeptidase; AltName: Full=Peptidase S; AltName: Full=Proline aminopeptidase; EC=3.4.11.5; AltName: Full=Prolyl aminopeptidase;
FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.
CATALYTIC ACTIVITY: Release of an N-terminal amino acid, Xaa-|- Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
CATALYTIC ACTIVITY: Release of N-terminal proline from a peptide.
COFACTOR: Binds 2 zinc ions per subunit. One zinc ion is tightly bound and essential for enzyme activity, while the second metal coordination site can be occupied by zinc, magnesium or manganese to give enzymes of different activities (By similarity).
SUBUNIT: Homohexamer.
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the peptidase M17 family.

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): LAP3
CDC HuGE Published Literature: LAP3

-  MalaCards Disease Associations
  MalaCards Gene Search: LAP3
Diseases sorted by gene-association score: bacterial vaginosis (9), trichomoniasis (4), granulocytopenia (3), vaginal disease (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 84.17 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 972.30 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -70.90162-0.438 Picture PostScript Text
3' UTR -75.90378-0.201 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011356 - Peptidase_M17
IPR000819 - Peptidase_M17_C
IPR023042 - Peptidase_M17_cytosol_amino
IPR008283 - Peptidase_M17_N

Pfam Domains:
PF00883 - Cytosol aminopeptidase family, catalytic domain
PF02789 - Cytosol aminopeptidase family, N-terminal domain

SCOP Domains:
52949 - Macro domain-like
53187 - Zn-dependent exopeptidases

ModBase Predicted Comparative 3D Structure on P28838
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserNo orthologNo ortholog
Gene Details  Gene Details  
Gene Sorter  Gene Sorter  
  EnsemblFlyBase  
  Protein SequenceProtein Sequence  
  AlignmentAlignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004177 aminopeptidase activity
GO:0008233 peptidase activity
GO:0008235 metalloexopeptidase activity
GO:0016787 hydrolase activity
GO:0030145 manganese ion binding
GO:0046872 metal ion binding

Biological Process:
GO:0006508 proteolysis
GO:0019538 protein metabolic process

Cellular Component:
GO:0005622 intracellular
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005802 trans-Golgi network
GO:0005829 cytosol
GO:0005925 focal adhesion
GO:0030496 midbody
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AF061738 - Homo sapiens leucine aminopeptidase mRNA, complete cds.
FJ224308 - Homo sapiens epididymis secretory protein Li 106 (HEL-S-106) mRNA, complete cds.
LF206837 - JP 2014500723-A/14340: Polycomb-Associated Non-Coding RNAs.
JD196079 - Sequence 177103 from Patent EP1572962.
BC065564 - Homo sapiens leucine aminopeptidase 3, mRNA (cDNA clone MGC:70735 IMAGE:6144134), complete cds.
JD056730 - Sequence 37754 from Patent EP1572962.
CR457128 - Homo sapiens full open reading frame cDNA clone RZPDo834G0914D for gene LAP3, leucine aminopeptidase 3; complete cds, incl. stopcodon.
KJ898809 - Synthetic construct Homo sapiens clone ccsbBroadEn_08203 LAP3 gene, encodes complete protein.
AK022055 - Homo sapiens cDNA FLJ11993 fis, clone HEMBB1001429, highly similar to Cytosol aminopeptidase (EC 3.4.11.1).
AK298613 - Homo sapiens cDNA FLJ61331 complete cds, highly similar to Cytosol aminopeptidase (EC 3.4.11.1).
AK307723 - Homo sapiens cDNA, FLJ97671.
AK225511 - Homo sapiens mRNA for leucine aminopeptidase variant, clone: KAT03244.
BC006199 - Homo sapiens leucine aminopeptidase 3, mRNA (cDNA clone IMAGE:2821948), partial cds.
LF358405 - JP 2014500723-A/165908: Polycomb-Associated Non-Coding RNAs.
AK298790 - Homo sapiens cDNA FLJ54122 complete cds, highly similar to Cytosol aminopeptidase (EC 3.4.11.1).
LF358407 - JP 2014500723-A/165910: Polycomb-Associated Non-Coding RNAs.
LF358409 - JP 2014500723-A/165912: Polycomb-Associated Non-Coding RNAs.
LF358410 - JP 2014500723-A/165913: Polycomb-Associated Non-Coding RNAs.
LF358411 - JP 2014500723-A/165914: Polycomb-Associated Non-Coding RNAs.
LF358412 - JP 2014500723-A/165915: Polycomb-Associated Non-Coding RNAs.
AK130293 - Homo sapiens cDNA FLJ26783 fis, clone PRS03956, highly similar to Cytosol aminopeptidase (EC 3.4.11.1).
LF358414 - JP 2014500723-A/165917: Polycomb-Associated Non-Coding RNAs.
JD049096 - Sequence 30120 from Patent EP1572962.
JD176628 - Sequence 157652 from Patent EP1572962.
MA442414 - JP 2018138019-A/14340: Polycomb-Associated Non-Coding RNAs.
MA593982 - JP 2018138019-A/165908: Polycomb-Associated Non-Coding RNAs.
MA593984 - JP 2018138019-A/165910: Polycomb-Associated Non-Coding RNAs.
MA593986 - JP 2018138019-A/165912: Polycomb-Associated Non-Coding RNAs.
MA593987 - JP 2018138019-A/165913: Polycomb-Associated Non-Coding RNAs.
MA593988 - JP 2018138019-A/165914: Polycomb-Associated Non-Coding RNAs.
MA593989 - JP 2018138019-A/165915: Polycomb-Associated Non-Coding RNAs.
MA593991 - JP 2018138019-A/165917: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00330 - Arginine and proline metabolism
hsa00480 - Glutathione metabolism
hsa01100 - Metabolic pathways

-  Other Names for This Gene
  Alternate Gene Symbols: AMPL_HUMAN, B3KMQ3, LAPEP, NM_015907, NP_056991, P28838, PEPS, Q6IAM6, Q6P0L6, Q9UQE3
UCSC ID: uc003gph.1
RefSeq Accession: NM_015907
Protein: P28838 (aka AMPL_HUMAN)
CCDS: CCDS3422.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_015907.2
exon count: 13CDS single in 3' UTR: no RNA size: 2100
ORF size: 1560CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3320.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.