Human Gene MED22 (uc004cdc.3) Description and Page Index
  Description: Homo sapiens mediator complex subunit 22 (MED22), transcript variant b, mRNA.
RefSeq Summary (NM_133640): This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013].
Transcript (Including UTRs)
   Position: hg19 chr9:136,207,755-136,214,972 Size: 7,218 Total Exon Count: 5 Strand: -
Coding Region
   Position: hg19 chr9:136,208,355-136,213,517 Size: 5,163 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:136,207,755-136,214,972)mRNA (may differ from genome)Protein (200 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
H-INVHGNCHPRDLynxMGIneXtProt
OMIMPubMedReactomeStanford SOURCETreefamUniProtKB
Wikipedia

-  Comments and Description Text from UniProtKB
  ID: MED22_HUMAN
DESCRIPTION: RecName: Full=Mediator of RNA polymerase II transcription subunit 22; AltName: Full=Mediator complex subunit 22; AltName: Full=Surfeit locus protein 5; Short=Surf-5;
FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
SUBUNIT: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
INTERACTION: Q9P086:MED11; NbExp=3; IntAct=EBI-394687, EBI-394704; Q9CQI9:Med30 (xeno); NbExp=4; IntAct=EBI-394687, EBI-309220;
SUBCELLULAR LOCATION: Nucleus (Probable).
SIMILARITY: Belongs to the Mediator complex subunit 22 family.

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): MED22
CDC HuGE Published Literature: MED22

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.62 RPKM in Uterus
Total median expression: 303.13 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -122.80234-0.525 Picture PostScript Text
3' UTR -241.40600-0.402 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR009332 - Mediator_Med22

Pfam Domains:
PF06179 - Surfeit locus protein 5 subunit 22 of Mediator complex

ModBase Predicted Comparative 3D Structure on Q15528
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
 Protein SequenceProtein Sequence   
 AlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001104 RNA polymerase II transcription cofactor activity
GO:0005515 protein binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0016592 mediator complex


-  Descriptions from all associated GenBank mRNAs
  AK124518 - Homo sapiens cDNA FLJ42527 fis, clone BRACE3001754, highly similar to Surfeit locus protein 5.
LF205599 - JP 2014500723-A/13102: Polycomb-Associated Non-Coding RNAs.
AJ224360 - Homo sapiens surf5c mRNA, clone 10.9.
AJ224359 - Homo sapiens surf5b mRNA, clone L5.
BC024225 - Homo sapiens mediator complex subunit 22, mRNA (cDNA clone MGC:2563 IMAGE:3009749), complete cds.
BC040111 - Homo sapiens mediator complex subunit 22, mRNA (cDNA clone MGC:48682 IMAGE:6056006), complete cds.
AK126069 - Homo sapiens cDNA FLJ44081 fis, clone TESTI4040363, highly similar to Surfeit locus protein 5.
AJ224358 - Homo sapiens surf5a mRNA, clone 1de.
JD230241 - Sequence 211265 from Patent EP1572962.
JD244150 - Sequence 225174 from Patent EP1572962.
JD293654 - Sequence 274678 from Patent EP1572962.
JD162302 - Sequence 143326 from Patent EP1572962.
JD483350 - Sequence 464374 from Patent EP1572962.
JD248373 - Sequence 229397 from Patent EP1572962.
JD498693 - Sequence 479717 from Patent EP1572962.
JD370749 - Sequence 351773 from Patent EP1572962.
JD275939 - Sequence 256963 from Patent EP1572962.
JD384661 - Sequence 365685 from Patent EP1572962.
JD565258 - Sequence 546282 from Patent EP1572962.
JD200484 - Sequence 181508 from Patent EP1572962.
JD126675 - Sequence 107699 from Patent EP1572962.
JD257882 - Sequence 238906 from Patent EP1572962.
AB463864 - Synthetic construct DNA, clone: pF1KB6878, Homo sapiens MED22 gene for mediator complex subunit 22, without stop codon, in Flexi system.
JD474734 - Sequence 455758 from Patent EP1572962.
JD343928 - Sequence 324952 from Patent EP1572962.
JD418683 - Sequence 399707 from Patent EP1572962.
JD310697 - Sequence 291721 from Patent EP1572962.
JD038644 - Sequence 19668 from Patent EP1572962.
X85178 - H.sapiens mRNA for SURF-5 protein.
HQ448554 - Synthetic construct Homo sapiens clone IMAGE:100071990; CCSB011303_01 surfeit 5 (MED22) gene, encodes complete protein.
KJ892232 - Synthetic construct Homo sapiens clone ccsbBroadEn_01626 MED22 gene, encodes complete protein.
CU691814 - Synthetic construct Homo sapiens gateway clone IMAGE:100021044 5' read MED22 mRNA.
LF346443 - JP 2014500723-A/153946: Polycomb-Associated Non-Coding RNAs.
JD409159 - Sequence 390183 from Patent EP1572962.
JD331257 - Sequence 312281 from Patent EP1572962.
JD462615 - Sequence 443639 from Patent EP1572962.
MA582020 - JP 2018138019-A/153946: Polycomb-Associated Non-Coding RNAs.
MA441176 - JP 2018138019-A/13102: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q15528 (Reactome details) participates in the following event(s):

R-HSA-381309 PPARG:RXRA heterodimer binds to fatty acid-like ligands
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
R-HSA-1266738 Developmental Biology

-  Other Names for This Gene
  Alternate Gene Symbols: B3KW83, B3KWX4, MED22_HUMAN, NM_133640, NP_598395, O76072, Q15528, Q5T8U0, SURF5
UCSC ID: uc004cdc.3
RefSeq Accession: NM_133640
Protein: Q15528 (aka MED22_HUMAN)
CCDS: CCDS6963.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_133640.3
exon count: 5CDS single in 3' UTR: no RNA size: 1448
ORF size: 603CDS single in intron: no Alignment % ID: 99.93
txCdsPredict score: 1406.00frame shift in genome: no % Coverage: 99.24
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.