Human Gene INTS8 (uc003yhb.4) Description and Page Index
  Description: Homo sapiens integrator complex subunit 8 (INTS8), transcript variant 1, mRNA.
RefSeq Summary (NM_017864): This gene encodes a subunit of the Integrator complex which is involved in the cleavage of small nuclear RNAs U1 and U2 within the nucleus. The encoded protein associates with RNA polymerase II and is recruited to the U1 and U2 small nuclear RNA genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012].
Transcript (Including UTRs)
   Position: hg19 chr8:95,835,518-95,892,721 Size: 57,204 Total Exon Count: 27 Strand: +
Coding Region
   Position: hg19 chr8:95,835,660-95,892,462 Size: 56,803 Coding Exon Count: 27 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:95,835,518-95,892,721)mRNA (may differ from genome)Protein (995 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMGI
neXtProtOMIMPubMedReactomeStanford SOURCEUniProtKB
Wikipedia

-  Comments and Description Text from UniProtKB
  ID: INT8_HUMAN
DESCRIPTION: RecName: Full=Integrator complex subunit 8; Short=Int8; AltName: Full=Protein kaonashi-1;
FUNCTION: Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
SUBUNIT: Belongs to the multiprotein complex Integrator, at least composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, CPSF3L/INTS11 and INTS12.
SUBCELLULAR LOCATION: Nucleus (Probable).
SIMILARITY: Contains 4 TPR repeats.
SEQUENCE CAUTION: Sequence=AAH50536.1; Type=Frameshift; Positions=985; Sequence=BAA91238.1; Type=Erroneous initiation; Sequence=BAA91671.1; Type=Erroneous initiation;

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): INTS8
CDC HuGE Published Literature: INTS8

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 17.34 RPKM in Testis
Total median expression: 398.37 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -62.10142-0.437 Picture PostScript Text
3' UTR -41.10259-0.159 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011990 - TPR-like_helical

SCOP Domains:
48439 - Protein prenylyltransferase

ModBase Predicted Comparative 3D Structure on Q75QN2
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserGenome BrowserNo ortholog
Gene Details  Gene DetailsGene Details 
Gene Sorter  Gene SorterGene Sorter 
  EnsemblFlyBaseWormBase 
  Protein SequenceProtein SequenceProtein Sequence 
  AlignmentAlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0016180 snRNA processing
GO:0034472 snRNA 3'-end processing
GO:0042795 snRNA transcription from RNA polymerase II promoter

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0032039 integrator complex


-  Descriptions from all associated GenBank mRNAs
  BC099915 - Homo sapiens cDNA clone IMAGE:5295321, containing frame-shift errors.
AB161944 - Homo sapiens mRNA for KAONASHI protein 1, complete cds.
BC136754 - Homo sapiens integrator complex subunit 8, mRNA (cDNA clone MGC:168367 IMAGE:9020744), complete cds.
BC144396 - Homo sapiens cDNA clone IMAGE:9052917.
BC144397 - Homo sapiens cDNA clone IMAGE:9052918.
AK091278 - Homo sapiens cDNA FLJ33959 fis, clone CTONG2018808.
AX746892 - Sequence 417 from Patent EP1308459.
BX538203 - Homo sapiens mRNA; cDNA DKFZp686P06215 (from clone DKFZp686P06215).
AK001403 - Homo sapiens cDNA FLJ10541 fis, clone NT2RP2001381.
BC064915 - Homo sapiens integrator complex subunit 8, mRNA (cDNA clone IMAGE:6069156), partial cds.
BC050536 - Homo sapiens integrator complex subunit 8, mRNA (cDNA clone IMAGE:5535173), partial cds.
AK000537 - Homo sapiens cDNA FLJ20530 fis, clone KAT10776.
AK225533 - Homo sapiens mRNA for hypothetical protein LOC55656 variant, clone: KAT10776.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q75QN2 (Reactome details) participates in the following event(s):

R-HSA-6814885 CBCAP complex binds 7-methylguanosine cap of snRNA
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: B2RN92, C8orf52, INT8_HUMAN, NM_017864, NP_060334, Q5RKZ3, Q6P1R5, Q75QN2, Q7Z314, Q9NVS6, Q9NWY7, uc003yhb.3
UCSC ID: uc003yhb.4
RefSeq Accession: NM_017864
Protein: Q75QN2 (aka INT8_HUMAN)
CCDS: CCDS34925.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_017864.3
exon count: 27CDS single in 3' UTR: no RNA size: 3409
ORF size: 2988CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 6176.00frame shift in genome: no % Coverage: 99.41
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.