Human Gene MRM2 (uc003slm.3)
  Description: Homo sapiens FtsJ RNA methyltransferase homolog 2 (E. coli) (MRM2), mRNA.
RefSeq Summary (NM_013393): The protein encoded by this gene is a member of the S-adenosylmethionine-binding protein family. It is a nucleolar protein and it may be involved in the processing and modification of rRNA. This gene has been suggested to be involved in cell cycle control and DNA repair. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
   Position: hg19 chr7:2,273,926-2,281,833 Size: 7,908 Total Exon Count: 3 Strand: -
Coding Region
   Position: hg19 chr7:2,274,757-2,281,804 Size: 7,048 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:2,273,926-2,281,833)mRNA (may differ from genome)Protein (246 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGeneCardsGeneNetworkH-INV
HPRDLynxMGIneXtProtOMIMPubMed
ReactomeTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RRMJ2_HUMAN
DESCRIPTION: RecName: Full=Putative ribosomal RNA methyltransferase 2; EC=2.1.1.-; AltName: Full=Protein ftsJ homolog 2; AltName: Full=rRNA (uridine-2'-O-)-methyltransferase;
FUNCTION: Probable methyltransferase.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + rRNA = S-adenosyl-L- homocysteine + rRNA containing 2'-O-methyluridine.
SUBCELLULAR LOCATION: Nucleus, nucleolus.
TISSUE SPECIFICITY: Widely expressed, with highest expression in muscle, placenta, and heart.
SIMILARITY: Belongs to the methyltransferase superfamily. RlmE family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 21.41 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 354.68 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -9.8029-0.338 Picture PostScript Text
3' UTR -274.62831-0.330 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR015507 - rRNA-MeTfrase_E
IPR002877 - rRNA_MeTrfase_FtsJ_dom

Pfam Domains:
PF01728 - FtsJ-like methyltransferase

SCOP Domains:
53335 - S-adenosyl-L-methionine-dependent methyltransferases

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2NYU - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9UI43
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserGenome BrowserNo ortholog
Gene Details  Gene DetailsGene Details 
Gene Sorter  Gene SorterGene Sorter 
  EnsemblFlyBaseWormBase 
  Protein SequenceProtein SequenceProtein Sequence 
  AlignmentAlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008168 methyltransferase activity
GO:0008650 rRNA (uridine-2'-O-)-methyltransferase activity
GO:0016740 transferase activity

Biological Process:
GO:0000451 rRNA 2'-O-methylation
GO:0001510 RNA methylation
GO:0006364 rRNA processing
GO:0008283 cell proliferation
GO:0031167 rRNA methylation
GO:0032259 methylation

Cellular Component:
GO:0005730 nucleolus
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix


-  Descriptions from all associated GenBank mRNAs
  BC017106 - Homo sapiens FtsJ homolog 2 (E. coli), mRNA (cDNA clone IMAGE:3537517).
AF093415 - Homo sapiens cell division protein FtsJ (FJH1) mRNA, complete cds.
EU794658 - Homo sapiens epididymis luminal protein 97 (HEL97) mRNA, complete cds.
LF384063 - JP 2014500723-A/191566: Polycomb-Associated Non-Coding RNAs.
CR749618 - Homo sapiens mRNA; cDNA DKFZp686J14194 (from clone DKFZp686J14194).
BC017181 - Homo sapiens FtsJ homolog 2 (E. coli), mRNA (cDNA clone IMAGE:3343278), partial cds.
AK302060 - Homo sapiens cDNA FLJ61455 complete cds, highly similar to ribosomal RNA methyltransferase 2(EC 2.1.1.-).
JD301396 - Sequence 282420 from Patent EP1572962.
JD327967 - Sequence 308991 from Patent EP1572962.
JD038288 - Sequence 19312 from Patent EP1572962.
AK024635 - Homo sapiens cDNA: FLJ20982 fis, clone ADSU02018, highly similar to AF093415 Homo sapiens cell division protein FtsJ (FJH1) mRNA.
JD286573 - Sequence 267597 from Patent EP1572962.
JD286572 - Sequence 267596 from Patent EP1572962.
JD530569 - Sequence 511593 from Patent EP1572962.
JD557732 - Sequence 538756 from Patent EP1572962.
JD170453 - Sequence 151477 from Patent EP1572962.
JD378206 - Sequence 359230 from Patent EP1572962.
JD101516 - Sequence 82540 from Patent EP1572962.
JD116801 - Sequence 97825 from Patent EP1572962.
JD048293 - Sequence 29317 from Patent EP1572962.
JD109589 - Sequence 90613 from Patent EP1572962.
BC114514 - Homo sapiens FtsJ homolog 2 (E. coli), mRNA (cDNA clone MGC:138620 IMAGE:40031660), complete cds.
BC114564 - Homo sapiens FtsJ homolog 2 (E. coli), mRNA (cDNA clone IMAGE:40031661), partial cds.
JD449623 - Sequence 430647 from Patent EP1572962.
JD207255 - Sequence 188279 from Patent EP1572962.
JD440148 - Sequence 421172 from Patent EP1572962.
JD561170 - Sequence 542194 from Patent EP1572962.
JD037238 - Sequence 18262 from Patent EP1572962.
JD268647 - Sequence 249671 from Patent EP1572962.
JD314092 - Sequence 295116 from Patent EP1572962.
JD089279 - Sequence 70303 from Patent EP1572962.
JD100661 - Sequence 81685 from Patent EP1572962.
KJ898756 - Synthetic construct Homo sapiens clone ccsbBroadEn_08150 FTSJ2 gene, encodes complete protein.
JD345235 - Sequence 326259 from Patent EP1572962.
JD038548 - Sequence 19572 from Patent EP1572962.
JD245886 - Sequence 226910 from Patent EP1572962.
JD402657 - Sequence 383681 from Patent EP1572962.
JD148043 - Sequence 129067 from Patent EP1572962.
JD399186 - Sequence 380210 from Patent EP1572962.
JD291235 - Sequence 272259 from Patent EP1572962.
JD225039 - Sequence 206063 from Patent EP1572962.
JD027140 - Sequence 8164 from Patent EP1572962.
JD030101 - Sequence 11125 from Patent EP1572962.
JD252422 - Sequence 233446 from Patent EP1572962.
JD026258 - Sequence 7282 from Patent EP1572962.
JD035293 - Sequence 16317 from Patent EP1572962.
LF360032 - JP 2014500723-A/167535: Polycomb-Associated Non-Coding RNAs.
JD024451 - Sequence 5475 from Patent EP1572962.
JD032279 - Sequence 13303 from Patent EP1572962.
MA619640 - JP 2018138019-A/191566: Polycomb-Associated Non-Coding RNAs.
MA595609 - JP 2018138019-A/167535: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9UI43 (Reactome details) participates in the following event(s):

R-HSA-6793127 MRM2 (FTSJ2) methylates uridine-1369 of 16S rRNA yielding 2'-O-methyluridine
R-HSA-6793080 rRNA modification in the mitochondrion
R-HSA-8868766 rRNA processing in the mitochondrion
R-HSA-72312 rRNA processing
R-HSA-8953854 Metabolism of RNA

-  Other Names for This Gene
  Alternate Gene Symbols: FJH1, FTSJ2, NM_013393, NP_037525, Q24JR8, Q9UI43, RRMJ2_HUMAN
UCSC ID: uc003slm.3
RefSeq Accession: NM_013393
Protein: Q9UI43 (aka RRMJ2_HUMAN)
CCDS: CCDS5328.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_013393.1
exon count: 3CDS single in 3' UTR: no RNA size: 1605
ORF size: 741CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1682.00frame shift in genome: no % Coverage: 99.75
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.