Description: Homo sapiens DPH2 homolog (S. cerevisiae) (DPH2), transcript variant 1, mRNA. RefSeq Summary (NM_001384): This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]. Transcript (Including UTRs) Position: hg19 chr1:44,435,653-44,439,043 Size: 3,391 Total Exon Count: 6 Strand: + Coding Region Position: hg19 chr1:44,435,848-44,438,211 Size: 2,364 Coding Exon Count: 6
ID:DPH2_HUMAN DESCRIPTION: RecName: Full=Diphthamide biosynthesis protein 2; AltName: Full=DPH2 homolog; Short=HsDph2; AltName: Full=Diphthamide biosynthesis protein 2 homolog-like 2; Short=DPH2-like 2; FUNCTION: Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (By similarity). SUBUNIT: Interacts with DPH1 (By similarity). TISSUE SPECIFICITY: Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, speen, thymus, prostate, ovary and lymphocytes. SIMILARITY: Belongs to the DPH1/DPH2 family. DPH2 subfamily.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF01866 - Putative diphthamide synthesis protein
ModBase Predicted Comparative 3D Structure on Q9BQC3
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK315506 - Homo sapiens cDNA, FLJ96571, Homo sapiens diptheria toxin resistance protein required fordiphthamide biosynthesis-like 2 (S. cerevisiae) (DPH2L2), mRNA. BC003181 - Homo sapiens DPH2 homolog (S. cerevisiae), mRNA (cDNA clone MGC:4428 IMAGE:2958202), complete cds. JD303583 - Sequence 284607 from Patent EP1572962. AK297933 - Homo sapiens cDNA FLJ55730 complete cds, highly similar to Homo sapiens DPH2 homolog (DPH2), transcript variant 1, mRNA. AK091303 - Homo sapiens cDNA FLJ33984 fis, clone DFNES2005540, highly similar to Homo sapiens DPH2 homolog (DPH2), transcript variant 1, mRNA. AX746904 - Sequence 429 from Patent EP1308459. BC001389 - Homo sapiens DPH2 homolog (S. cerevisiae), mRNA (cDNA clone MGC:1559 IMAGE:3051455), complete cds. BC016956 - Homo sapiens DPH2 homolog (S. cerevisiae), mRNA (cDNA clone MGC:21520 IMAGE:3900854), complete cds. AF053003 - Homo sapiens diphthamide biosynthesis protein-2 (DPH2) mRNA, complete cds. JD390821 - Sequence 371845 from Patent EP1572962. JD226489 - Sequence 207513 from Patent EP1572962. JD364253 - Sequence 345277 from Patent EP1572962. KJ896721 - Synthetic construct Homo sapiens clone ccsbBroadEn_06115 DPH2 gene, encodes complete protein. BT007431 - Homo sapiens diptheria toxin resistance protein required for diphthamide biosynthesis-like 2 (S. cerevisiae) mRNA, complete cds. DQ594577 - Homo sapiens piRNA piR-60689, complete sequence. JD437345 - Sequence 418369 from Patent EP1572962. JD400375 - Sequence 381399 from Patent EP1572962. JD193017 - Sequence 174041 from Patent EP1572962. JD474155 - Sequence 455179 from Patent EP1572962. JD326552 - Sequence 307576 from Patent EP1572962. JD555063 - Sequence 536087 from Patent EP1572962. JD162025 - Sequence 143049 from Patent EP1572962. JD134270 - Sequence 115294 from Patent EP1572962. JD457094 - Sequence 438118 from Patent EP1572962. JD173260 - Sequence 154284 from Patent EP1572962. JD209015 - Sequence 190039 from Patent EP1572962. JD385399 - Sequence 366423 from Patent EP1572962. JD109514 - Sequence 90538 from Patent EP1572962. JD227411 - Sequence 208435 from Patent EP1572962. JD491519 - Sequence 472543 from Patent EP1572962. JD281094 - Sequence 262118 from Patent EP1572962. JD333781 - Sequence 314805 from Patent EP1572962. JD516827 - Sequence 497851 from Patent EP1572962. JD218763 - Sequence 199787 from Patent EP1572962. JD040836 - Sequence 21860 from Patent EP1572962. JD228073 - Sequence 209097 from Patent EP1572962. JD523865 - Sequence 504889 from Patent EP1572962. JD233557 - Sequence 214581 from Patent EP1572962. JD549658 - Sequence 530682 from Patent EP1572962. JD189238 - Sequence 170262 from Patent EP1572962. JD088378 - Sequence 69402 from Patent EP1572962. JD553716 - Sequence 534740 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9BQC3 (Reactome details) participates in the following event(s):
R-HSA-5358494 DPH2 transfers a 3-amino-3-carboxypropyl group from AdoMet to residue 715 of nascent EEF2 R-HSA-5358493 Synthesis of diphthamide-EEF2 R-HSA-163841 Gamma carboxylation, hypusine formation and arylsulfatase activation R-HSA-597592 Post-translational protein modification R-HSA-392499 Metabolism of proteins